Oral Administration of Lactobacillus Acidophilus Restores Nitric Oxide Level in Diabetic Rats

Harisa, G. I.; Taha, E. I.; Khalil, A. F.; Salem, M. M.
July 2009
Australian Journal of Basic & Applied Sciences;2009, Vol. 3 Issue 3, p2963
Academic Journal
Nitric oxide (NO) is an important regulator of many physiological and pathophysiological processes. Excessive formation of reactive oxygen metabolites (ROMs ) is responsible for disruption of No in diabetic. The objectives of this work was to formulate Lactobacillus acidophilus (LA ) in a gastric resistance formulation and to investigate the effect of administration of LA alone or in combination with acarbose (AC) on plasma NO level and some oxidative stress parameters in diabetic rats . Granules containing LA with and without A C have been prepared us ing Eudragit L 100 as drug release retarding polymer. Colony forming unit (CFU) has been done after the granulation process and after placing in acidic medium to as sure the viability of the LA. The rats were divided into control, diabetic, diabetic received AC, diabetic received LA and diabetics received AC plus LA groups . Fasting blood sugar (FBS), glycosylated hemoglobin (HbA 1c %), triacylglycerol (TAG) level, Arylesterase (ARE) activity of paraoxonase enzyme, malondialdehyde (MDA) and plasma nitrate were measured. The results obtained from this study revealed that treatment with LA alone or in combination with AC significantly decrease the elevated FBS level, HbA1c %, plasma TAG and MDA compared to diabetic rats . The same treatment elevated t he ARE activity compared to that of diabetic group. Administration of LA alone or with AC showed a significant elevation in p la s ma nitrate level compared with that of diabetic group. Finally we concluded that, LA and AC supplementation exert hypoglycemic, hypotriglyceridemic and antioxidant effects. These effects are responsible for restoration of NO level.


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