Investigation of Non-Linear Adaptive Responses and Split Dose Recovery Induced by Ionizing Radiation in Three Human Epithelial Derived Cell Lines

Ryan, Lorna A.; Seymour, Colin B.; Mothersill, Carmel E.
October 2009
Dose-Response;2009, Vol. 7 Issue 4, p292
Academic Journal
Two almost completely exclusive fields in radiobiology deal with splitting doses of radiation and comparing the effect to a similar total dose given in one exposure. In radiotherapy, dose "fractionation" is used to "spare" normal tissue and in the low dose field, the adaptive response is well documented as a phenomenon where a small "priming" dose administered before the larger "challenge " dose reduces the effect of the large dose. There have been very few studies where these fields overlap, thus it is not possible to ascertain whether common or distinct mechanisms underlie both phenomena but this is certainly an interesting question and relevant to our understanding of high and low dose radiobiology. This paper presents data for three human cell lines with varying p53 status and radiation responses, treated at a range of times between first and second dose and for 3 different first doses (0.1, 0.5 and 2Gy). The data show that time between doses is critical. Protective (adaptive) effects were seen in each cell line but most prominently in the malignant HT 29 cell line. Surprisingly none of the cell lines showed pronounced split dose recovery. This suggests different mechanisms may underlie the two phenomena.


Related Articles

  • H2AX phosphorylation screen of cells from radiosensitive cancer patients reveals a novel DNA double-strand break repair cellular phenotype. Vasireddy, R. S.; Sprung, C. N.; Cempaka, N. L.; Chao, M.; McKay, M. J. // British Journal of Cancer;5/11/2010, Vol. 102 Issue 10, p1511 

    Background:About 1–5% of cancer patients suffer from significant normal tissue reactions as a result of radiotherapy (RT). It is not possible at this time to predict how most patients’ normal tissues will respond to RT. DNA repair dysfunction is implicated in sensitivity to RT...

  • Detection of Chromosomal Instability in Bystander Cells after Si490-Ion Irradiation. Ponnaiya, Brian; Suzuki, Masao; Tsuruoka, Chirzuru; Uchihori, Yukio; Wei, Ying; Hei, Tom K. // Radiation Research;Sep2011, Vol. 176 Issue 3, p280 

    There is increasing evidence that two of the biological effects associated with low-dose ionizing radiation, genomic instability and bystander responses, may be linked. To verify and validate the link between the two phenomena, the ability of Si490 ions (high-energy particles associated with...

  • Some Principles of Radiobiology: A Selective Review. Malkinson, Frederick D. // Journal of Investigative Dermatology;Jul81, Vol. 77 Issue 1, p32 

    This article presents principles of radiobiology. Radiobiology is the study of the effects of ionizing radiation on living things. These effects may be investigated in the whole organism and/or at the tissue, cellular, and molecular levels. Studies of the biological changes induced by ionizing...

  • DNA DAMAGE AND THE GOVERNING DYNAMICS OF STEM CELL RADIOBIOLOGY. Tomuleasa, C. I.; Foris, V.; Soriţãu, Olga; Páll, Emöke; Dicu, T.; Lung-Illes, V.; Brie, Ioana; Kacsó, G. // Annals of the Romanian Society for Cell Biology;2009, Vol. 14 Issue 2, p13 

    Stem cells are undifferentiated cells that are defined by their ability to both self-renew and to differentiate in order to produce mature progeny cells, including both non-renewing progenitors and terminally differentiated effector cells. Human mesenchymal stem cells can potentially be used in...

  • Evolution of Physico-Mathematical Models in Radiobiology and their Application in Ionizing Radiation Therapies. Garzón, O.; Plazas, M. C.; Salazar, E. J. // Tecciencia;Jul-Dec2014, Vol. 9 Issue 17, p15 

    Radiobiology is the science of studying the interaction between radiation and biological tissues and the effects of the first on the latter. In this paper, we present various Physico-Mathematical models used in Radiobiology, which have been developed in order to compare and quantify the...

  • CDDO-Me Protects Normal Lung and Breast Epithelial Cells but Not Cancer Cells from Radiation. El-Ashmawy, Mariam; Delgado, Oliver; Cardentey, Agnelio; Wright, Woodring E.; Shay, Jerry W. // PLoS ONE;Dec2014, Vol. 9 Issue 12, p1 

    Although radiation therapy is commonly used for treatment for many human diseases including cancer, ionizing radiation produces reactive oxygen species that can damage both cancer and healthy cells. Synthetic triterpenoids, including CDDO-Me, act as anti-inflammatory and antioxidant modulators...

  • Prostate epithelial cell lines form spheroids with evidence of glandular differentiation in three-dimensional Matrigel cultures. Lang, S H; Sharrard, R M; Stark, M; Villette, J M; Maitland, N J // British Journal of Cancer;8/15/2001, Vol. 85 Issue 4, p590 

    Normal (PNT2-C2) and metastatic (PC-3) prostate cell lines were grown in Matrigel to observe the effects on morphology and phenotype in comparison to monolayer culture. In monolayer cultures, PNT2-C2 showed typical round/cuboidal epithelial morphology, with tight cell associations, whereas in...

  • Effect of AKT inhibition on epithelial-mesenchymal transition and ZEB1-potentiated radiotherapy in nasopharyngeal carcinoma. WEIGUO CHEN; SIPEI WU; GONG ZHANG; WENJUN WANG; YONGSHENG SHI // Oncology Letters;2013, Vol. 6 Issue 5, p1234 

    Radiotherapy is a major treatment regime for nasopharyngeal carcinoma (NPC), and although initial responses to a complete course of radiation are good, recurrence and metastasis are frequent events. A number of previous studies have observed that ionizing radiation (IR) may enhance the migratory...

  • 53BP1 and MDC1 foci formation in HT-1080 cells for low- and high-LET microbeam irradiations. Mosconi, Marita; Giesen, Ulrich; Langner, Frank; Mielke, Christian; Dalla Rosa, Ilaria; Dirks, Wilhelm // Radiation & Environmental Biophysics;Aug2011, Vol. 50 Issue 3, p345 

    n improved assessment of the biological effects and related risks of low doses of ionizing radiation is currently an important issue in radiation biology. Irradiations using microbeams are particularly well suited for precise and localized dose depositions, whereas recombinant cell lines with...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics