TITLE

Salmonella Infection of Gallbladder Epithelial Cells Drives Local Inflammation and Injury in a Model of Acute Typhoid Fever

AUTHOR(S)
Menendez, Alfredo; Arena, Ellen T.; Guttman, Julian A.; Thorson, Lisa; Vallance, Bruce A.; Vogl, Wayne; Finlay, B. Brett
PUB. DATE
December 2009
SOURCE
Journal of Infectious Diseases;12/1/2009, Vol. 200 Issue 11, p1703
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The gallbladder is often colonized by Salmonella during typhoid fever, yet little is known about bacterial pathogenesis in this organ. With use of a mouse model of acute typhoid fever, we demonstrate that Salmonella infect gallbladder epithelial cells in vivo. Bacteria in the gallbladder showed a unique behavior as they replicated within gallbladder epithelial cells and remained confined to those cells without translocating to the mucosa. Infected gallbladders showed histopathological damage characterized by destruction of the epithelium and massive infiltration of neutrophils, accompanied by a local increase of proinflammatory cytokines. Damage was determined by the ability of Salmonella to invade gallbladder epithelial cells and was independent of high numbers of replication-competent, although invasion-deficient, bacteria in the lumen. Our results establish gallbladder epithelial cells as a novel niche for in vivo replication of Salmonella and reveal the involvement of these cells in the pathogenesis of Salmonella in the gallbladder during the course of acute typhoid fever.
ACCESSION #
45566195

 

Related Articles

  • The Microbiological and Clinical Characteristics of Invasive Salmonella in Gallbladders from Cholecystectomy Patients in Kathmandu, Nepal. Dongol, Sabina; Thompson, Corinne N.; Clare, Simon; Thieu Nga, Tran Vu; Duy, Pham Thanh; Karkey, Abhilasha; Arjyal, Amit; Koirala, Samir; Khatri, Nely Shrestha; Maskey, Pukar; Poudel, Sanjay; Jaiswal, Vijay Kumar; Vaidya, Sujan; Dougan, Gordon; Farrar, Jeremy J.; Dolecek, Christiane; Basnyat, Buddha; Baker, Stephen // PLoS ONE;Oct2012, Vol. 7 Issue 10, Special section p1 

    Gallbladder carriage of invasive Salmonella is considered fundamental in sustaining typhoid fever transmission. Bile and tissue was obtained from 1,377 individuals undergoing cholecystectomy in Kathmandu to investigate the prevalence, characteristics and relevance of invasive Salmonella in the...

  • Salmonella Transiently Reside in Luminal Neutrophils in the Inflamed Gut. Loetscher, Yvonne; Wieser, Andreas; Lengefeld, Jette; Kaiser, Patrick; Schubert, Sören; Heikenwalder, Mathias; Hardt, Wolf-Dietrich; Stecher, Bärbel // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    Background: Enteric pathogens need to grow efficiently in the gut lumen in order to cause disease and ensure transmission. The interior of the gut forms a complex environment comprising the mucosal surface area and the inner gut lumen with epithelial cell debris and food particles. Recruitment...

  • Salmonella Transiently Reside in Luminal Neutrophils in the Inflamed Gut. Loetscher, Yvonne; Wieser, Andreas; Lengefeld, Jette; Kaiser, Patrick; Schubert, S�ren; Heikenwalder, Mathias; Hardt, Wolf-Dietrich; Stecher, B�rbel // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    Background: Enteric pathogens need to grow efficiently in the gut lumen in order to cause disease and ensure transmission. The interior of the gut forms a complex environment comprising the mucosal surface area and the inner gut lumen with epithelial cell debris and food particles. Recruitment...

  • Helicobacter felis-induced gastritis was suppressed in mice overexpressing thioredoxin-1. Kawasaki, Kimio; Nishio, Akiyoshi; Nakamura, Hajime; Uchida, Kazushige; Fukui, Toshiro; Ohana, Masaya; Yoshizawa, Hazuki; Ohashi, Shinya; Tamaki, Hiroyuki; Matsuura, Minoru; Asada, Masanori; Nishi, Toshiki; Nakase, Hiroshi; Toyokuni, Shinya; Liu, Wenrui; Yodoi, Junji; Okazaki, Kazuichi; Chiba, Tsutomu // Laboratory Investigation (00236837);Sep2005, Vol. 85 Issue 9, p1104 

    Thioredoxin-1 (TRX-1) is a redox-active protein involved in scavenging reactive oxygen species and regulating redox-sensitive transcription factors. TRX-1 is induced in various inflammatory conditions and shows cytoprotective action. We investigated the roles of TRX-1 in the host defense...

  • Repeated daily exposure to 2 ppm nitrogen dioxide upregulates the expression of IL-5, IL- 10, IL- 13, and ICAM-1 in the bronchial epithelium of healthy human airways. Pathmanathan, S.; Krishna, M. T.; Blomberg, A.; Helleday, R.; Kelly, F. J.; Sandstrom, T.; Holgate, S. T.; Wilson, S. J.; Frew, A. J. // Occupational & Environmental Medicine;Nov2003, Vol. 60 Issue 11, p892 

    Background: Repeated daily exposure of healthy human subjects to NO[sub2] induces an acute airway inflammatory response characterised by neutrophil influx in the bronchial mucosa Aims: To assess the expression of NF-κB, cytokines, and ICAM-1 in the bronchial epithelium. Methods: Twelve...

  • MICROSCOPIC STUDY OF THE GALL BLADDER OF THE CHUKAR PARTRIDGE (ALECTORIS CHUKAR). Mobini, B. // Bulgarian Journal of Veterinary Medicine;Jun2012, Vol. 15 Issue 2, p73 

    A study on the microscopic anatomy of the gall bladder was conducted in 8 female and 8 male twenty-week-old healthy Iranian chukar partridges (Alectoris chukar). The gall bladder was composed of tunica mucosa, tunica muscularis and tunica serosa or tunica adventitia. The tunica mucosa was mainly...

  • Effects on Endometrial Elafin Expression.  // Fertility Weekly;8/8/2011, p12 

    The article presents a study on the effects of endometrial elafin expression in women. The study aims to compare expression and localization of elafin in the endometrium of women with and without hydrosalpinx. The findings suggest that women with hydrosalpinx has reduced antimicrobial and...

  • Neutrophil-derived JAML inhibits repair of intestinal epithelial injury during acute inflammation. Weber, D A; Sumagin, R; McCall, I C; Leoni, G; Neumann, P A; Andargachew, R; Brazil, J C; Medina-Contreras, O; Denning, T L; Nusrat, A; Parkos, C A // Mucosal Immunology (1933-0219);Sep2014, Vol. 7 Issue 5, p1221 

    Neutrophil transepithelial migration (TEM) during acute inflammation is associated with mucosal injury. Using models of acute mucosal injury in vitro and in vivo, we describe a new mechanism by which neutrophils infiltrating the intestinal mucosa disrupt epithelial homeostasis. We report that...

  • Leukocyte–Epithelial Interactions and Mucosal Homeostasis. Matthews, Jason D.; Weight, Caroline M.; Parkos, Charles A. // Toxicologic Pathology;Jan2014, Vol. 42 Issue 1, p91 

    Many common inflammatory disorders are characterized by the infiltration of neutrophils across epithelial lined (mucosal) surfaces resulting in disruption of critical barrier function that protects from microbes and noxious agents. In such conditions, disease symptoms are complex but directly...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics