TITLE

Tetraacylated Lipopolysaccharide of Yersinia pestis Can Inhibit Multiple Toll-Like Receptor—Mediated Signaling Pathways in Human Dendritic Cells

AUTHOR(S)
Telepnev, Maxim V.; Klimpel, Gary R.; Haithcoat, Judith; Knirel, Yuriy A.; Anisimov, Andrey P.; Motin, Vladimir L.
PUB. DATE
December 2009
SOURCE
Journal of Infectious Diseases;12/1/2009, Vol. 200 Issue 11, p1694
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background. Yersinia pestis, the causative agent of plague, showed a temperature-dependent change in lipid A composition, with a reduced degree of acylation when bacteria were grown at 37°C (tetraacylated) versus ambient temperature (hexaacylated). Methods. Human monocytes and monocyte-derived dendritic cells (DCs) were exposed to Y. pestis grown at 26°C or 37°C, to their corresponding lipopolysaccharides (LPS-26°C or LPS-37°C), and to ligands of different Toll-like receptors (TLRs), such as LPS from Escherichia coli (TLR4), lipoprotein (TLR2), polyinosinic-polycytidylic acid (poly-IC) (TLR9), and their combinations. Production of cytokines was measured, along with expression of surface markers of DC maturation. Results. Y. pestis grown at 37°C or LPS-37°C induced much lower production of cytokines (such as tumor necrosis factor a and interleukins 1b, 10, and 12) by DCs than did Y. pestis grown at 26°C or LPS-26°C. Expression of the surface markers HLA-DR, CD86, and CD40 by DCs was also reduced in response to treatment with LPS- 37°C compared with LPS-26°C. Pretreatment of DCs with LPS-37°C inhibited subsequent stimulation with LPS- 26°C, control LPS from E. coli, lipoprotein, or poly-IC. Conclusions. LPS-37°C can inhibit stimulation of DCs not only via TLR4 signaling but also via TLR2 and TLR9.
ACCESSION #
45566194

 

Related Articles

  • Monocyte Function in the Fetus and the Preterm Neonate: Immaturity Combined with Functional Impairment. Iliodromiti, Zoe; Anastasiadis, Anastasis; Varras, Michail; Pappa, Kalliopi I.; Siristatidis, Charalambos; Bakoulas, Vassilios; Mastorakos, George; Vrachnis, Nikolaos // Mediators of Inflammation;2013, Vol. 2013, p1 

    It is well known that the innate immunity system, involving the contribution of monocytes and macrophages, may dysfunction in fetuses and preterm neonates. Monocytes are capable of differentiating into dendritic cells (DCs) or into mucosal macrophages during certain infections and of producing...

  • Serum IL-10 from systemic lupus erythematosus patients suppresses the differentiation and function of monocyte-derived dendritic cells. Sun, Zhida; Zhang, Rong; Wang, Huijuan; Jiang, Pengtao; Zhang, Jiangquan; Zhang, Mingshun; Gu, Lei; Yang, Xiaofan; Zhang, Miaojia; Ji, Xiaohui // Journal of Biomedical Research;Nov2012, Vol. 26 Issue 6, p456 

    Abstract: The role played by cytokines, other than interferon (IFN)-α, in the differentiation and function of dendritic cells (DCs) in systemic lupus erythematosus (SLE), remains unclear. Serum interleukin-10 (IL-10) levels are generally elevated in SLE patients, which might modulate the...

  • Plasma Cytokine and Endotoxin Levels Correlate with Survival in Patients with the Sepsis Syndrome. Casey, Larry C.; Balk, Robert A.; Bone, Roger C. // Annals of Internal Medicine;10/15/93, Vol. 119 Issue 8, p771 

    Presents a study which determined whether plasma tumor necrosis factor-alpha, interleukin-1beta, interleukin-6, and lipopolysaccharide are detectable in patients with sepsis syndrome. Correlation of plasma cytokine and endotoxin levels with patient survival; Methods; Results and discussion.

  • Lymphocyte-polarized DC1s. Berk, Erik; Kalinski, Pawel // OncoImmunology;Nov2012, Vol. 1 Issue 8, p1444 

    Activated lymphocytes secrete dendritic cell (DC)-activating cytokines including tumor necrosis factor α and interferon γ, and induce Type-1-polarized DCs (DC1s). Lymphocyte-polarized DC1s secrete high levels of biologically active interleukin-12 (IL-12p70) and CXCL10 and show enhanced...

  • Monocytes, Peripheral Blood Mononuclear Cells, and THP-1 Cells Exhibit Different Cytokine Expression Patterns following Stimulation with Lipopolysaccharide. Schildberger, Anita; Rossmanith, Eva; Eichhorn, Tanja; Strassl, Katharina; Weber, Viktoria // Mediators of Inflammation;2013, Vol. 2013, p1 

    THP-1 cells are widely applied to mimic monocytes in cell culture models. In this study, we compared the cytokine release from THP-1, peripheral blood mononuclear cells (PBMC), monocytes, or whole blood after stimulation with lipopolysaccharide (LPS) and investigated the consequences of...

  • Neutralization of Endotoxin by a Phospholipid Emulsion in Healthy Volunteers. Gordon, Bruce R.; Parker, Thomas S.; Levine, Daniel M.; Feuerbach, Fred; Saal, Stuart D.; Sloan, Betty-Jane; Cindy Chu; Stenzel, Kurt H.; Parrillo, Joseph E.; Rubin, Albert L. // Journal of Infectious Diseases;5/1/2005, Vol. 191 Issue 9, p1515 

    Background. An approach to endotoxin (lipopolysaccharide [LPS]) blockade makes use of the ability of lipoproteins, via surface phospholipids, to bind and neutralize LPS. The aim of the present study was to determine whether the intravenous administration of a protein-free, phospholipid-rich...

  • Residual Endotoxin Contaminations in Recombinant Proteins Are Sufficient to Activate Human CD1c+ Dendritic Cells. Schwarz, Harald; Schmittner, Maria; Duschl, Albert; Horejs-Hoeck, Jutta // PLoS ONE;Dec2014, Vol. 9 Issue 12, p1 

    Many commercially available recombinant proteins are produced in Escherichia coli, and most suppliers guarantee contamination levels of less than 1 endotoxin unit (EU). When we analysed commercially available proteins for their endotoxin content, we found contamination levels in the same range...

  • A crucial role for IL-6 in the CNS of rats during fever induced by the injection of live E. coli. Soares, Denis; Figueiredo, Maria; Martins, Juliano; Machado, Renes; Sorgi, Carlos; Faciolli, Lucia; Alves-Filho, José; Cunha, Fernando; Souza, Glória // Medical Microbiology & Immunology;Feb2012, Vol. 201 Issue 1, p47 

    Interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6 have been established as important mediators of fever induced by lipopolysaccharide (LPS) from Gram-negative bacteria. Whether these pro-inflammatory cytokines are also important in mediating fever induced by live bacteria...

  • Porphyromonas Gingivalis and E-coli Induce Different Cytokine Production Patterns in Pregnant Women. Faas, Marijke M.; Kunnen, Alina; Dekker, Daphne C.; Harmsen, Hermie J. M.; Aarnoudse, Jan G.; Abbas, Frank; De Vos, Paul; Van Pampus, Maria G. // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    Objective: Pregnant individuals of many species, including humans, are more sensitive to various bacteria or their products as compared with non-pregnant individuals. Pregnant individuals also respond differently to different bacteria or their products. Therefore, in the present study, we...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics