TITLE

Hard x-ray phase contrast imaging of black lipid membranes

AUTHOR(S)
Beerlink, A.; Mell, M.; Tolkiehn, M.; Salditt, T.
PUB. DATE
November 2009
SOURCE
Applied Physics Letters;11/16/2009, Vol. 95 Issue 20, p203703
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We report hard x-ray phase contrast imaging of black lipid membranes, freely suspended over a micromachined aperture in an aqueous solution. Biomolecular and organic substances can thus be probed in hydrated environments by parallel beam propagation imaging, using coherent multi-kilo-electronvolt x-ray radiation. The width of the thinning film can be resolved from analysis of the intensity fringes in the Fresnel diffraction regime down to about 200 nm. The thinning process, in which solvent is expelled from the space in between two opposing monolayers, is monitored, and the domain walls between coexisting domains of swollen and thinned membrane patches are characterized.
ACCESSION #
45366460

 

Related Articles

  • Atomic force microscopy of model lipid membranes. Morandat, Sandrine; Azouzi, Slim; Beauvais, Estelle; Mastouri, Amira; El Kirat, Karim // Analytical & Bioanalytical Chemistry;Feb2013, Vol. 405 Issue 5, p1445 

    Supported lipid bilayers (SLBs) are biomimetic model systems that are now widely used to address the biophysical and biochemical properties of biological membranes. Two main methods are usually employed to form SLBs: the transfer of two successive monolayers by Langmuir-Blodgett or...

  • Spontaneous curvature of fluid vesicles induced by trans-bilayer sugar asymmetry. Döbereiner, Hans-Günther; Selchow, Olaf; Lipowsky, Reinhard // European Biophysics Journal;1999, Vol. 28 Issue 2, p174 

    Abstract We present measurements of the effective spontaneous curvature of fluid lipid bilayers as a function of trans-bilayer asymmetry. Experiments are performed on micrometer-scale vesicles in sugar solutions with varying species across the membrane. There are two effects leading to a...

  • On the theory of electrostatic interaction of neutral lipid bilayers separated by thin water film. Leikin, S. // Journal of Chemical Physics;10/1/1991, Vol. 95 Issue 7, p5224 

    A simple analytical solution is obtained for the screened Coulombic force between neutral lipid bilayers separated by thin water film within a model formally analogous to the ‘‘spherical model’’ for Ising systems. This model accounts for constraints on the surface...

  • Characterization of the Interaction of Hemolytic Lectin CEL-III from the Marine Invertebrate, Cucumaria echinata, with Artificial Lipid Membranes: Involvement of Neutral Sphingoglycolipids in the Pore-Forming Process1. Hatakeyama, Tomomitsu; Sato, Taiji; Taira, Etsuyo; Kuwahara, Hiromiki; Niidome, Takuro; Aoyagi, Haruhiko // Journal of Biochemistry;1999, Vol. 125 Issue 2, p277 

    The hemolytic lectin, CEL-III, is a Ca2+-dependent, galactose/N-acetylgalactosamine-specific lectin purified from the marine invertebrate, Cucumaria echinata (Holothuroidea). After binding to specific carbohydrates on the erythrocyte surface, CEL-III forms ion-permeable pores by oligomerizing in...

  • Interaction of Amphotericin B with Cholesterol in Monolayers, Aqueous Solutions, and Phospholipid Bilayers. Saka, Yukiko; Mita, Tomoyoshi // Journal of Biochemistry;1998, Vol. 123 Issue 5, p798 

    The interaction of amphotericin B (AmB) with cholesterol was investigated in monolayers, aqueous solutions, and phospholipid vesicles. When AmB was mixed with cholesterol, it formed a stable monolayer, implying complex formation in which the stoichiometry was primarily 1:1 AmB: cholesterol....

  • Tilt modulus of a lipid monolayer. May, S.; Kozlovsky, Y.; Ben-Shaul, A.; Kozlov, M. M. // European Physical Journal E -- Soft Matter;Jul2004, Vol. 14 Issue 3, p299 

    Focuses on the tilt modulus of a lipid monolayer. Information on tilt free energy and elastic modulus; Calculation of the tilt modulus; Details on 3 phenomenological model for the effective tilt modulus.

  • New Aspect of the Spontaneous Formation of a Bilayer Lipid Membrane. Fujiwara, Hisashi; Fujihara, Masayuki; Koyama, Takahiro; Ishiwata, Takashi // AIP Conference Proceedings;2004, Vol. 708 Issue 1, p724 

    An artificial lipid bilayer in planar form, well known as bilayer lipid membrane (BLM), spontaneously forms from a lipid droplet (diphytanoyl phosphatidylcholine in n-decane and chloroform in this work) in an aperture of a thin partition in aqueous solution. The thinning dynamics of the lipid...

  • Morphological Effects Induced In Vitro by Propranolol on Human Erythrocytes. Suwalsky, Mario; Zambrano, Pablo; Villena, Fernando; Manrique-Moreno, Marcela; Gallardo, María; Jemiola-Rzeminska, Malgorzata; Strzalka, Kazimierz; Edwards, Ana; Mennickent, Sigrid; Dukes, Nathan // Journal of Membrane Biology;Aug2015, Vol. 248 Issue 4, p683 

    Despite the extended use and well-documented information, there are insufficient reports concerning the effects of propranolol on the structure and functions of cell membranes, particularly those of human erythrocytes. Aimed to better understand the molecular mechanisms of its interactions with...

  • Electrowetting on dielectric-based microfluidics for integrated lipid bilayer formation and measurement. Poulos, Jason L.; Nelson, Wyatt C.; Tae-Joon Jeon; Chang-Jin Kim; Schmidt, Jacob J. // Applied Physics Letters;7/6/2009, Vol. 95 Issue 1, p013706 

    We present a microfluidic platform for the formation and electrical measurement of lipid bilayer membranes. Using electrowetting on dielectric (EWOD), two or more aqueous droplets surrounded by a lipid-containing organic phase were manipulated into contact to form a lipid bilayer at their...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics