TITLE

Determination of ganglioside composition and structure in human brain hemangioma by chip-based nanoelectrospray ionization tandem mass spectrometry

AUTHOR(S)
Schiopu, Catalin; Flangea, Corina; Capitan, Florina; Serb, Alina; Vukelić, Željka; Kalanj-Bognar, Svjetlana; Sisu, Eugen; Przybylski, Michael; Zamfir, Alina D.
PUB. DATE
December 2009
SOURCE
Analytical & Bioanalytical Chemistry;Dec2009, Vol. 395 Issue 8, p2465
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We report here on a preliminary investigation of ganglioside composition and structure in human hemangioma, a benign tumor in the frontal cortex (HFC) in comparison to normal frontal cortex (NFC) tissue using for the first time advanced mass spectrometric methods based on fully automated chip-nanoelectrospray (nanoESI) high-capacity ion trap (HCT) and collision-induced dissociation (CID). The high ionization efficiency, sensitivity and reproducibility provided by the chip-nanoESI approach allowed for a reliable MS-based ganglioside comparative assay. Unlike NFC, ganglioside mixture extracted from HFC was found dominated by species of short glycan chains exhibiting lower overall sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected. Interestingly, none of these trisialylated forms was detected in NFC, suggesting that such components might selectively be associated with HFC. Unlike the case of previously investigated high malignancy gliosarcoma, in HFC one modified O-Ac-GD2 and one modified O-Ac-GM4 gangliosides were observed. This aspect suggests that these O-acetylated structures could be associated with cerebral tumors having reduced malignancy grade. Fragmentation analysis by CID in MS2 mode using as precursors the ions corresponding to GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data corroborating for the first time the presence of the common GT1a and GT1b isomers and the incidence of unusual GT1c and GT1d glycoforms in brain hemangioma tumor. [Figure not available: see fulltext.]
ACCESSION #
45363033

 

Related Articles

  • Femtosecond pump-probe experiments on trapped flavin: Optical control of dissociation. Guyon, Laurent; Tabarin, Thibault; Thuillier, Benoı⁁t; Antoine, Rodolphe; Broyer, Michel; Boutou, Véronique; Wolf, Jean-Pierre; Dugourd, Philippe // Journal of Chemical Physics;2/21/2008, Vol. 128 Issue 7, p075103 

    Femtosecond pump-probe experiments are performed on flavin biomolecules isolated in an ion trap. Mass spectra of the photoinduced fragments show that the fragmentation pathways can be modified using two-color two-photon excitation. In particular, when an infrared probe pulse (810 nm) is added...

  • Dissociation of alkane ionized molecules. Afrosimov, V. V.; Baranova, L. A.; Basalaev, A. A.; Panov, M. N.; Smirnov, O. V.; Tulub, A. V. // Technical Physics;Mar2010, Vol. 55 Issue 3, p357 

    The subject of investigation is the fragmentation of variously charged molecular ions arising in col-lisions of several kiloelectronvolt H+, He2+, and Ar6+ ions with molecules of the simplest alkanes (from methane to butane). Using the method of time-of-flight mass spectrometry, the formation...

  • Individual b2 ion fragmentation profiles combined with AspN digestion improve N-terminal peptide sequencing. Winter, Dominic; Lehmann, Wolf D. // Analytical & Bioanalytical Chemistry;Mar2009, Vol. 393 Issue 6/7, p1587 

    The N terminus of peptides generated by AspN is restricted to about 40 dipeptide motifs starting with D or E. These motifs are visible upon collision-induced dissociation (CID) as b2 ions, which are often the most abundant low-mass fragment ions. It was observed that b2 ions are accompanied by a...

  • Improved identification of O-linked glycopeptides from ETD data with optimized scoring for different charge states and cleavage specificities. Darula, Zsuzsa; Chalkley, Robert; Lynn, Aenoch; Baker, Peter; Medzihradszky, Katalin // Amino Acids;Jul2011, Vol. 41 Issue 2, p321 

    This article describes the effect of re-interrogation of electron-transfer dissociation (ETD) data with newly developed analytical tools. MS/MS-based characterization of O-linked glycopeptides is discussed using data acquired from a complex mixture of O-linked glycopeptides, featuring mucin core...

  • Fragmentation of singly charged adenine induced by neutral fluorine beam impact at 3 keV. Chen, L.; Brédy, R.; Bernard, J.; Montagne, G.; Allouche, A. R.; Martin, S. // Journal of Chemical Physics;9/20/2011, Vol. 135 Issue 11, p114309 

    The fragmentation scheme of singly charged adenine molecule (H5C5N5+) has been studied via neutral fluorine impact at 3 keV. By analyzing in correlation the kinetic energy loss of the scattered projectile F- produced in single charge transfer process and the mass of the charged fragments, the...

  • Electron ionization of SiCl4. King, Simon J.; Price, Stephen D. // Journal of Chemical Physics;2/21/2011, Vol. 134 Issue 7, p074311 

    Relative partial ionization cross sections (PICS) for the formation of fragment ions following electron ionization of SiCl4, in the electron energy range 30-200 eV, have been determined using time-of-flight mass spectrometry coupled with an ion coincidence technique. By this method, the...

  • Dissociative electron attachment to triflates. Ptasinska, Sylwia; Gschliesser, David; Bartl, Peter; Janik, Ireneusz; Scheier, Paul; Denifl, Stephan // Journal of Chemical Physics;12/7/2011, Vol. 135 Issue 21, p214309 

    Gas phase studies of dissociative electron attachment to simple alkyl (CF3SO3CH3) and aryl (C6H5SO3CF3 and CF3SO3C6H4CH3) triflates, model molecules of nonionic photoacid generators for modern lithographic applications, were performed. The fragmentation pathways under electron impact below 10 eV...

  • Competitive fragmentation pathways of acetic acid dimer explored by synchrotron VUV photoionization mass spectrometry and electronic structure calculations. Guan, Jiwen; Hu, Yongjun; Zou, Hao; Cao, Lanlan; Liu, Fuyi; Shan, Xiaobin; Sheng, Liusi // Journal of Chemical Physics;9/28/2012, Vol. 137 Issue 12, p124308 

    In present study, photoionization and dissociation of acetic acid dimers have been studied with the synchrotron vacuum ultraviolet photoionization mass spectrometry and theoretical calculations. Besides the intense signal corresponding to protonated cluster ions (CH3COOH)n·H+, the feature...

  • Electron-impact-induced fragmentation of proline molecule. Vukstich, V.; Romanova, L.; Megela, I.; Papp, A.; Snegursky, A. // Technical Physics Letters;Oct2014, Vol. 40 Issue 10, p901 

    The formation of ionized products upon single and dissociative ionization of proline (CHNO) molecule by electron impact at high (11.5 MeV) and low (below 150 eV) energies has been studied using mass-spectrometric techniques. The mass-spectra of proline molecule have been obtained and interpreted...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics