TITLE

Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine

AUTHOR(S)
Ming-Zhou Zhang; Min-Zi Wang; Zong-Lun Chen; Jie-Hong Fang; Mei-Ming Fang; Jun Liu; Xiao-Ping Yu
PUB. DATE
December 2009
SOURCE
Analytical & Bioanalytical Chemistry;Dec2009, Vol. 395 Issue 8, p2591
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A multianalyte lateral-flow immunochromatographic technique using colloidal gold-labeled polyclonal antibodies was developed for the rapid simultaneous detection of clenbuterol and ractopamine. The assay procedure could be accomplished within 5 min, and the results of this qualitative one-step assay were evaluated visually according to whether test lines appeared or not. When applied to the swine urines, the detection limit and the half maximal inhibitory concentration (IC50) of the test strip under an optical density scanner were calculated to be 0.1 ± 0.01 ng mL−1 and 0.1 ± 0.01 ng mL−1, 0.56 ± 0.08 ng mL−1, and 0.71 ± 0.06 ng mL−1, respectively, the cut-off levels with the naked eye of 1 ng mL−1 and 1 ng mL−1 for clenbuterol and ractopamine were observed. Parallel analysis of swine urine samples with clenbuterol and ractopamine showed comparable results obtained from the multianalyte lateral-flow test strip and GC-MS. Therefore, the described multianalyte lateral-flow test strip can be used as a reliable, rapid, and cost-effective on-site screening technique for the simultaneous determination of clenbuterol and ractopamine residues in swine urine. [Figure not available: see fulltext.]
ACCESSION #
45363029

 

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