Analysis of Clonality and Antibiotic Resistance among Early Clinical Isolates of Enterococcus faecium in the United States

Galloway-Peña, Jessica R.; Nallapareddy, Sreedhar R.; Arias, Cesar A.; Eliopoulos, George M.; Murray, Barbara E.
November 2009
Journal of Infectious Diseases;11/15/2009, Vol. 200 Issue 10, p1566
Academic Journal
Background. The Enterococcus faecium genogroup, referred to as clonal complex 17 (CC17), seems to possess multiple determinants that increase its ability to survive and cause disease in nosocomial environments. Methods. Using 53 clinical and geographically diverse US E. faecium isolates dating from 1971 to 1994, we determined the multilocus sequence type; the presence of 16 putative virulence genes (hylEfm, espEfm, and fms genes); resistance to ampicillin (AMP) and vancomycin (VAN); and high-level resistance to gentamicin and streptomycin. Results. Overall, 16 different sequence types (STs), mostly CC17 isolates, were identified in 9 different regions of the United States. The earliest CC17 isolates were part of an outbreak that occurred in 1982 in Richmond, Virginia. The characteristics of CC17 isolates included increases in resistance to AMP, the presence of hylEfm and espEfm, emergence of resistance to VAN, and the presence of at least 13 of 14 fms genes. Eight of 41 of the early isolates with resistance to AMP, however, were not in CC17. Conclusions. Although not all early US AMP isolates were clonally related, E. faecium CC17 isolates have been circulating in the United States since at least 1982 and appear to have progressively acquired additional virulence and antibiotic resistance determinants, perhaps explaining the recent success of this species in the hospital environment.


Related Articles

  • Vankomisine Dirençli Enterococcus faecium’a BaÄŸlı Olarak Prostetik Kapak Endokarditi GeliÅŸen Olgu. CESUR, Salih; ALTIN, Nilgün; KESKİN, Atilla; YAPAR TOROS, Göknur; SOLGUN, Gülkan; KATIRCIOĞLU, S. Fehmi; ŞENCAN, İrfan // Ortadogu Medical Journal / Ortadogu Tip Dergisi;Dec2013, Vol. 5 Issue 4, p233 

    Endocarditis due to vancomycine resistant enteroccoci is very rare and it is commonly developed as an nosocomial infection. In this manuscript, a 28 years old female who developed prostetic valve endocarditis due to vancomycin resistant Enterococcus faecium was presented and the literature was...

  • High-level Aminoglycoside Resistance and Reduced Susceptibility to Vancomycin in Nosocomial Enterococci. Adhikari, Luna // Journal of Global Infectious Diseases;Sep2010, Vol. 2 Issue 3, p231 

    Objectives: The objectives of the present study were to identify the species of enterococci isolated from nosocomial infections and to determine the antibiotic susceptibility pattern with reference to high-level aminoglycosides and vancomycin. Materials and Methods: Enterococci were isolated...

  • Intestinal Colonization with Enterococcus faecium Does Not Influence Pulmonary Defense against Pseudomonas aeruginosa in Mice. Leendertse, Masja; Willems, Rob J. L.; Giebelen, Ida A. J.; Roelofs, Joris J. T. H.; Top, Janetta; Bonten, Marc J. M.; van der Poll, Tom // PLoS ONE;2009, Vol. 4 Issue 8, p1 

    Background: Enterococci, and especially multiresistant Enterococcus faecium, are increasingly found colonizing hospitalized patients. This increased prevalence of colonization is not only associated with an increased prevalence of infections caused by enterococci, but also by infections with...

  • Control of the Spread of Vancomycin- Resistant Enterococci in Hospitals. Mutters, Nico T.; Mersch-Sundermann, Volker; Mutters, Reinier; Brandt, Christian; Schneider-Brachert, Wulf; Frank, Uwe // Deutsches Aerzteblatt International;10/25/2013, Vol. 110 Issue 43, following p725 

    Background: The spread of vancomycin-resistant enterococci (VRE), particularly E. faecium, in hospitals leads to many cases of colonization, but only sporadic infections. Detailed and valid risk assessment is needed so that patients at risk can be protected from VRE infection. The principal aims...

  • Vancomycin-resistant Enterococcus faecium: Report of two cases. Ahuja, S.; Pandey, A.; Asthana, A. K.; Chauhan, K.; Ritika; Madan, M. // Indian Journal of Medical Microbiology;Jul-Sep2014, Vol. 32 Issue 3, p340 

    Vancomycin-resistant Enterococci (VRE), especially Enterococcus faecium has emerged as an important nososcomial pathogen and represents a serious threat to patients with impaired host defense. Early detection of patients colonised or infected with VRE is an essential component of any hospital...

  • Identification of vancomycin-resistant enterococci clones and inter-hospital spread during an outbreak in Taiwan. Sai-Cheong Lee; Mi-Si Wu; Hsiang-Ju Shih; Shu-Huan Huang; Meng-Jiun Chiou; Lai-Chu See; Liang-Kee Siu // BMC Infectious Diseases;2013, Vol. 13 Issue 1, p1 

    Background: In 2003, nosocomial infections caused by vancomycin-resistant enterococci (VRE) occurred rarely in Taiwan. Between 2003 and 2010, however, the average prevalence of vancomycin resistance among enterococci spp. increased from 2% to 16% in community hospitals and from 3% to 21% in...

  • Isolation and characterization of the first vancomycin-dependent Enterococcus from India. Banerjee, T.; Anupurba, S. // Indian Journal of Medical Microbiology;Jan-Mar2013, Vol. 31 Issue 1, p91 

    A letter to the editor is presented regarding the case of a 30-year-old female with isolation and characterization of vancomycin-dependent Enterococcus faecium (VDE).

  • Unprecedented nosocoimal spread os vancomycin-resistant Enterococcus faecium in a tertiary-care hospital in Switzerland. Senn, L.; Petignat, C.; Prod'hom, G.; Greub, G.; Basset, P.; Blanc, D. S.; Zanetti, G. // BMC Proceedings;Jan2011 Supplement 6, Vol. 5, pP22 

    An abstract of the article "Unprecedented nosocoimal spread os vancomycin-resistant Enterococcus faecium in a tertiary-care hospital in Switzerland," by biologists including L. Senn, C. Petignat and G. Greub is presented.

  • Antibiotic resistance pattern of Enterococci isolates from nosocomial infections in a tertiary care hospital in Eastern India. Chakraborty, Atreyi; Pal, Nishith K.; Sarkar, Soma; Gupta, Manideepa Sen // Journal of Natural Science, Biology & Medicine;Jul-Dec2015, Vol. 6 Issue 2, p394 

    Background: Resistance to commonly used antibiotics by Enterococci causing nosocomial infections is of concern, which necessitates judicious, responsible and evidence-based use of antibiotics. The present study was conducted to review the prevalence and identify therapeutic options for...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics