TITLE

Detection of angiotensin II type 1 receptor ligands by a cell-based assay

AUTHOR(S)
Grießner, Matthias; Bröker, Patrick; Lehmann, André; Ehrentreich-Förster, Eva; Bier, Frank F.
PUB. DATE
November 2009
SOURCE
Analytical & Bioanalytical Chemistry;Nov2009, Vol. 395 Issue 6, p1937
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This work describes a cell-based assay that does not depend on radioactivity or laboratory animals for the detection of ligands of angiotensin II type 1 receptor (AT1R). The assay makes use of stable transfected Chinese hamster ovary cells (CHO-AT1R) expressing the AT1R. A sequential saturation assay principle was used in which receptor binding sites of the CHO-AT1R cells are blocked by the analyte in a concentration-dependent manner. Afterwards, TAMRA-angiotensin II, a fluorescence-labeled ligand, was added to bind to the remaining free binding sites of the receptor. In consequence, the fluorescence signal determined is inversely proportional to the concentration of the analyte.
ACCESSION #
44917227

 

Related Articles

  • A yeast sensor of ligand binding. Tucker, Chandra L.; Fields, Stanley // Nature Biotechnology;Nov2001, Vol. 19 Issue 11, p1042 

    We describe a biosensor that reports the binding of small-molecule ligands to proteins as changes in growth of temperature-sensitive yeast. The yeast strains lack dihydrofolate reductase (DHFR) and are complemented by mouse DHFR containing a ligand-binding domain inserted in a flexible loop....

  • Effects of allisartan, a new AT1 receptor blocker, on blood pressure and end-organ damage in hypertensive animals. Ming-yue WU; Xiu-juan MA; Chu YANG; Xia TAO; Ai-jun LIU; Ding-feng SU; Jian-guo LIU // Acta Pharmacologica Sinica;Mar2009, Vol. 30 Issue 3, p307 

    AbstractAim:To investigate the effects of allisartan, a new angiotensin II type 1 (AT1) receptor antagonist, on blood pressure (BP) and end-organ damage (EOD) in hypertensive rats and dogs.Methods:First, a single dose of allisartan was given intragastrically to evaluate the BP reduction in...

  • Stress and Angiotensin II: Novel Therapeutic Opportunities. Armando, Ines; Seltzer, Alicia; Bregonzio, Claudia; Saavedra, Juan M. // Current Drug Targets - CNS & Neurological Disorders;Dec2003, Vol. 2 Issue 6, p413 

    Angiotensin II was initially described as a hormone of peripheral origin, the active end product of the Renin-Angiotensin System. The subsequent discovery that Angiotensin II was locally formed and selectively regulated in most organs indicated that tissue Angiotensin II systems might play...

  • Angiotensin II in atrial structural remodeling: the role of Ang II/JAK/STAT3 signaling pathway. Liuying Zheng; Xiaogang Jia; Cheng Zhang; Dong Wang; Zhongnan Cao; Jin Wang; Xinping Du // American Journal of Translational Research;2015, Vol. 7 Issue 6, p1021 

    Ang II/JAK/STAT3 signaling pathway is known to be involved in atrial remodeling associated with development and progression of atrial fibrillation. In the present study, we used in vivo animal model and human atrial specimens to further characterize the role of this pathway in the atrial...

  • Association between insertion/deletion polymorphism in angiotensin-converting enzyme gene and acute lung injury/acute respiratory distress syndrome: a meta-analysis. Akihisa Matsuda; Taro Kishi; Asha Jacob; Monowar Aziz; Ping Wang // BMC Medical Genetics;2012, Vol. 13 Issue 1, p76 

    Background: A previous meta-analysis reported a positive association between an insertion/deletion (I/D) polymorphism in the angiotensin-converting enzyme gene (ACE) and the risk of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Here, we updated this meta-analysis and...

  • Effects of lobeline, a nicotinic receptor ligand, on the cloned Kv1.5. Imju Jeong; Bok Hee Choi; Sang June Hahn // Pflugers Archiv European Journal of Physiology;Oct2010, Vol. 460 Issue 5, p851 

    The goal of the present study was to examine the effects of lobeline, an agonist at nicotinic receptors, on cloned Kv channels, Kv1.5, Kv3.1, Kv4.3, and human ether-a-gogo-related gene (HERG), which are stably expressed in Chinese hamster ovary (CHO) or human embryonic kidney 293 (HEK293) cells....

  • Characterization of a mutant type-1 angiotensin II receptor. Roche, Peter J.; Eddie, Lawrence W.; Coghlan, John P. // Kidney International;Dec1994, Vol. 46 Issue 6, p1580 

    We have used PCR to amplify the rat AT1 receptor from liver mRNA. The receptor DNA was subcloned into pcDNAI and a number of individual clones were transiently expressed in COS-7 cells. These individual receptors were characterized by binding of [125I]Sar1, Ile8-angiotensin II. It was found that...

  • Deciphering biased-agonism complexity reveals a new active AT1 receptor entity. Saulière, Aude; Bellot, Morgane; Paris, Hervé; Denis, Colette; Finana, Frédéric; Hansen, Jonas T; Altié, Marie-Françoise; Seguelas, Marie-Hélène; Pathak, Atul; Hansen, Jakob L; Sénard, Jean-Michel; Galés, Céline // Nature Chemical Biology;Jul2012, Vol. 8 Issue 7, p622 

    Functional selectivity of G protein-coupled receptor (GPCR) ligands toward different downstream signals has recently emerged as a general hallmark of this receptor class. However, pleiotropic and crosstalk signaling of GPCRs makes functional selectivity difficult to decode. To look from the...

  • Computational Approaches on Angiotensin Receptors and Their Ligands: Recent Developments and Results. Tuccinardi, Tiziano; Martinelli, Adriano // Current Medicinal Chemistry;2007, Vol. 14 Issue 29, p3105 

    Angiotensin II (AngII) is the major regulator of blood pressure, electrolyte balance, and some endocrine functions related to cardiovascular diseases. Moreover, it has been shown that AngII plays a role in various pathological situations involving tissue remodelling and in cancer. Two distinct...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics