Cerium oxide-chitosan based nanobiocomposite for food borne mycotoxin detection

Kaushik, Ajeet; Solanki, Pratima R.; Pandey, M. K.; Ahmad, Sharif; Malhotra, Bansi D.
October 2009
Applied Physics Letters;10/26/2009, Vol. 95 Issue 17, p173703
Academic Journal
Cerium oxide nanoparticles (NanoCeO2) and chitosan (CH) based nanobiocomposite film deposited onto indium-tin-oxide coated glass substrate has been used to coimmobilize rabbit immunoglobin (r-IgGs) and bovine serum albumin (BSA) for food borne mycotoxin [ochratoxin-A (OTA)] detection. Electrochemical studies reveal that presence of NanoCeO2 increases effective electro-active surface area of CH-NanoCeO2/indium tin oxide (ITO) nanobiocomposite resulting in high loading of r-IgGs. BSA/r-IgGs/CH-NanoCeO2/ITO immunoelectrode exhibits improved linearity (0.25–6.0 ng/dl), detection limit (0.25 ng/dl), response time (25 s), sensitivity (18 μA/ng dl-1 cm-2), and regression coefficient (r2∼0.997).


Related Articles

  • Correlation study between total calcium, ionized calcium, serum albumin and their significance with Vitamin D. Rajaraman, Srinath; Selvanayagam, Swaminathan; Chidambaram, Ramalingam // Journal of Experimental Sciences;2011, Vol. 2 Issue 12, p17 

    Calcium is an essential but controversial nutrient: there is no consensus on the level of human calcium requirement or the significance of calcium deficiency. Apart from providing rigidity to the skeleton, an alternate metabolic form of calcium viz., ionized calcium plays a key role in the...

  • The Influence of Serum Bovine and Egg Albumin on the Corrosion of Pure Aluminum in Sodium Hydroxide Solutions. Ahmed, Amal S. I.; Ghannem, Waffa A.; Abou Shahba, Rabab M.; El-Shenawy, Azza El-Sayed; Tantawy, Safaa M. // International Journal of Applied Environmental Sciences;2012, Vol. 7 Issue 3, p289 

    The Corrosion of pure Al was the subject of tremendous importance due to the increased industrial applications of Al metal. This study reported the results of open-circuit potential, potentiodynamic polarization and cyclic polarization measurements on the corrosion inhibition of pure Al in...

  • Determination of Serum Albumin in Blood by Constant-Current Coulometry Using Electrochemically Generated Oxidizers. Ziyatdinova, G. K.; Budnikov, G. K.; Pogorel'tsev, V. I. // Journal of Analytical Chemistry;Jul2004, Vol. 59 Issue 7, p659 

    A method is developed for the determination of human serum albumin by constant-current coulometry using electrochemically generated oxidizers. It is found that human serum albumin interacts with electrochemically generated bromine and iodine at a ratio of 1 : 73 and 1 : 64, respectively....

  • Interaction of Citrinin with Human Serum Albumin. Poór, Miklós; Lemli, Beáta; Bálint, Mónika; Hetényi, Csaba; Sali, Nikolett; Kőszegi, Tamás; Kunsági-Máté, Sándor // Toxins;Dec2015, Vol. 7 Issue 12, p5155 

    Citrinin (CIT) is a mycotoxin produced by several Aspergillus, Penicillium, and Monascus species. CIT occurs worldwide in different foods and drinks and causes health problems for humans and animals. Human serum albumin (HSA) is the most abundant plasma protein in human circulation. Albumin...

  • Comparison of using formaldehyde and carboxy methyl chitosan in preparation of Fe3O4 superparamagnetic nanoparticles-chitosan hydrogel network: Sorption behavior toward bovine serum albumin. Sadeghi, Meisam; Hanifpour, Fatemeh; Taheri, Reza; Javadian, Hamedreza; Ghasemi, Maryam // Process Safety & Environmental Protection: Transactions of the I;Jul2016, Vol. 102 Issue Part B, p119 

    A novel and cost effective method of bio-separation developed recently is magnetic separation technology. In this study, super paramagnetic Fe3O4 nanoparticles are used for separation of bovine serum albumin (BSA) protein from plasma/serum samples at optimized conditions. The synthesis of...

  • Artificial antigen synthesis and the development of polyclonal antibody-based immunoassay for citreoviridin determination. Zhuang, Zhen; Que, Shan; Gao, Yue; Yuan, Jun; Ye, Zhou; Du, Min; Lin, Guang; Liu, Li; Wang, Shi // World Journal of Microbiology & Biotechnology;Jan2014, Vol. 30 Issue 1, p343 

    Citreoviridin, a mycotoxin produced by Penicillium citreonigrum is a common contaminant of wide range of agri-products and detrimental to human and animal health. Therefore it is important to develop a rapid, sensitive, and specific immunoassay for citreoviridin detection. In this study,...

  • Influence of ultrasound irradiation on the adsorption of bovine serum albumin on copper. Pinto, Edilson M.; Soares, David M.; Brett, Christopher M. A. // Journal of Applied Electrochemistry;Nov2007, Vol. 37 Issue 11, p1367 

    The effect of irradiation by power ultrasound on the adsorption of proteins on copper has been investigated, using bovine serum albumin (BSA) as a model protein in pH 7 phosphate buffer solution. Open circuit potential measurements, cyclic voltammetry and electrochemical impedance spectroscopy...

  • Electrochemical Impedance Spectroscopy Study of the Adsorption Behavior of Bovine Serum Albumin at Biomimetic Calcium -- Phosphate Coating. Mohsen, Q.; Fadl-allah, Sahar A.; El-Shenawy, Nahla S. // International Journal of Electrochemical Science;2012, Vol. 7 Issue 5, p4510 

    The electrochemical impedance spectroscopy, EIS, was used to investigate the interfacial behavior of bovine serum albumin (BSA) on different titanium (Ti) surfaces at open circuit potential. Nano porous titanium oxide film, TiO2, was anodically formed on titanium from electrolyte containing 1M...

  • Toxicity of CdTe QDs with different sizes targeted to HSA investigated by two electrochemical methods. Xu, Zi-Qiang; Lai, Lu; Li, Dong-Wei; Li, Ran; Xiang, Chen; Jiang, Feng-Lei; Sun, Shao-Fa; Liu, Yi // Molecular Biology Reports;Feb2013, Vol. 40 Issue 2, p1009 

    QDs have large scale application in many important areas with potential of unintentional exposure to the environment or organism during processing of a nanotechnology containing product's life cycle. In this paper, two classical electrochemical methods, cyclic voltammetry and electrochemical...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics