TITLE

Panton-Valentine leukocidin in pediatric communityacquired Staphylococcus aureus infections

AUTHOR(S)
Papenburg, Jesse; Fontela, Patricia; Raynal, Lélia; Jetté3, Louise; Ismail, Johanne; Bekal, Sadjia; Al-Zahrani, Ibrahim; Quach, Caroline
PUB. DATE
October 2009
SOURCE
Clinical & Investigative Medicine;Oct2009, Vol. 32 Issue 5, pE352
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: Panton-Valentine Leukocidin (PVL) is an exotoxin produced by strains of Staphylococcus aureus (SA). Its importance as a virulence factor is controversial. We aim to further characterize the role of PVL in pediatric community-acquired SA infections. Methods: In a cohort study conducted from July to November 2006, we prospectively collected all strains of SA isolated at the Montreal Children's Hospital causing community-acquired infections in children aged 18 years or younger. The strains were analyzed for the presence of the PVL encoding genes by PCR and were phage typed. Strains resistant to methicillin or pvl+ were analyzed by pulsed-field gel electrophoresis. A medical chart review blinded to patient pvl status was performed to retrieve demographic and clinical data. Data were analyzed by logistic regression. Results: We identified 74 pediatric community-acquired SA infections. Nineteen strains (25.7%) were positive for the pvl genes. Four isolates (5.4%) were resistant to methicillin and three of these were pvl+. No predominant clone was identified by phage typing or pulsed field gel electrophoresis. pvl+ and pvl- infections were statistically similar for patient age, hospital admission, length of hospital stay, invasive disease, intravenous antibiotics and outcomes. pvl+ strains were more likely to cause abscesses (OR 20.79; 95% CI 4.93 - 87.58), less likely to cause superficial skin infections (OR 0.18; 95% CI 0.05 - 0.64) and less likely to be resistant to erythromycin (OR 0.048; 95% CI 0.004 - 0.52). Conclusions: In a clonally heterogeneous population of pediatric community-acquired SA infections, pvl+ strains were associated with abscess formation and erythromycin susceptibility, but not invasive disease.
ACCESSION #
44809934

 

Related Articles

  • A rapid screening method for Panton-Valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus belonging to multilocus sequence type 30 and its related clone using a combination of multiplex PCR and pulsed-field gel electrophoresis Ivan Reva; Wataru Higuchi; Tomomi Takano; Olga Singur; Kyoko Ozaki; Hirokazu Isobe; Shizuka Yabe; Kohei Saito; Tatiana Baranovich; Symaa Enany; Taketo Otsuka; Vladimir Potapov; Akihito Nishiyama; Tatsuo Yamamoto // Journal of Infection & Chemotherapy (Springer Science & Business;Apr2009, Vol. 15 Issue 2, p75 

    Abstract  Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA), which is often positive for Panton-Valentine leucocidin (PVL), is increasingly noted as an emerging pathogen worldwide. In Japan, PVLpositive CA-MRSA belonging to multilocus sequence type (ST) 30 has spread...

  • Prevalence and genetic relatedness of community-acquired methicillin-resistant Staphylococcus aureus in Israel. Regev-Yochay, G.; Carmeli, Y.; Raz, M.; Pinco, E.; Etienne, J.; Leavitt, A.; Rubinstein, E.; Navon-Venezia, S. // European Journal of Clinical Microbiology & Infectious Diseases;Nov2006, Vol. 25 Issue 11, p719 

    The aims of the study presented here were to determine the prevalence of Staphylococcus aureus carriage and, specifically, community-acquired methicillin-resistant S. aureus (CA-MRSA) carriage in children and their parents in Israel and to determine the genetic relatedness of these isolates. S....

  • Methicillin-Resistant Staphylococcus aureus Sequence Type 239-III, Ohio, USA, 2007-2009. Wang, Shu-Hua; Khan, Yosef; Hines, Lisa; Mediavilla, José R.; Zhang, Liangfen; Chen, Liang; Hoet, Armando; Bannerman, Tammy; Pancholi, Preeti; Robinson, D. Ashley; Kreiswirth, Barry N.; Stevenson, Kurt B. // Emerging Infectious Diseases;Oct2012, Vol. 18 Issue 10, p1557 

    Methicillin-resistant Staphylococcus aureus (MRSA) is a human pathogen that has diverse molecular heterogeneity. Most MRSA strains in the United States are pulsed-field gel electrophoresis USA100 sequence type (ST) 5 and USA300 ST8. Infections with MRSA ST239-III are common and found during...

  • Sequence-Based spa Typing as a Rapid Screening Method for the Areal and Nosocomial Outbreaks of MRSA. Narukawa, Munetoshi; Yasuoka, Akira; Note, Ryougo; Funada, Hisashi // Tohoku Journal of Experimental Medicine;Jul2009, Vol. 218 Issue 3, p207 

    Methicillin–resistant Staphylococcus aureus (MRSA) is the leading cause of nosocomial infection and MRSA outbreaks have become a major problem. Therefore, the rapid and accurate typing of MRSA isolates is important for epidemiological surveys and nosocomial infection control....

  • Evaluation of a commercial MRSA assay when multiple MRSA strains are causing epidemics. Sissonen, S.; Pasanen, T.; Salmenlinna, S.; Vuopio-Varkila, J.; Tarkka, E.; Vaara, M.; Tissari, P. // European Journal of Clinical Microbiology & Infectious Diseases;Oct2009, Vol. 28 Issue 10, p1271 

    Rapid and reliable diagnostic methods are needed to control methicillin-resistant Staphylococcus aureus (MRSA) transmission. We studied the BD GeneOhmâ„¢ MRSA Assay which is based on one specific amplification product at the junction of the right extremity sequence of the staphylococcal...

  • mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia. Ghaznavi-Rad, E.; Goering, R.; Nor Shamsudin, M.; Weng, P.; Sekawi, Z.; Tavakol, M.; Belkum, A.; Neela, V. // European Journal of Clinical Microbiology & Infectious Diseases;Nov2011, Vol. 30 Issue 11, p1365 

    The usefulness of mec-associated dru typing in the epidemiological analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Malaysia was investigated and compared with pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and spa and SCC mec typing. The...

  • Epidemiological Typing of Community-Acquired Methicillin-Resistant Staphylococcus aureus Isolates from Children in Taiwan. Chih-Chien Wang; Wen-Tsung Lo; Mong-Ling Chu; Siu, L. K. // Clinical Infectious Diseases;8/15/2004, Vol. 39 Issue 4, p481 

    Background. A 1400-bed tertiary medical center in northern Taiwan was used to conduct an epidemiological study of children hospitalized with community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infection during a 5-year period. Methods. Nineteen previously healthy children...

  • Transmission of MRSA CC398 strains between pig farms related by trade of animals. Espinosa-Gongora, C.; Broens, E. M.; Moodley, A.; Nielsen, J. P.; Guardabassi, L. // Veterinary Record: Journal of the British Veterinary Association;6/2/2012, Vol. 170 Issue 22, preceding p564 

    Meticillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 is a genetic lineage associated with livestock, especially pigs. The authors investigated the role of pig trade in the transmission of MRSA CC398 between farms using pulsed-field gel electrophoresis (PFGE), a highly...

  • Molecular Typing of Methicillin Resistant Staphylococcus aureus Strains Isolated from Cows and Farm Workers. ERDEM, Zeynep; TÜRKYILMAZ, Süheyla // Kafkas Universitesi Veteriner Fakultesi Dergisi;Nov/Dec2013, Vol. 19 Issue 6, p963 

    The aims of this study were to isolation and identification of methicillin resistant Staphylococcus aureus (MRSA) from mastiticcow milk and nasal swabs of cows and related workers, investigation presence of Panton-Valentine leucocidin (PVL) genes and molecular characteristics of these isolates....

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics