Self-assembly of cholesterol DNA at liquid crystal/aqueous interface and its application for DNA detection

Siok Lian Lai; Hartono, Deny; Kun-Lin Yang
October 2009
Applied Physics Letters;10/12/2009, Vol. 95 Issue 15, p153702
Academic Journal
In this letter, we report a strategy of detecting the DNA targets by using a thin layer of self-assembled cholesterol-labeled DNA probes at the liquid crystal (LC)/aqueous interface. When the system is exposed to 51 μg/ml of complementary DNA targets, the optical appearance of LC shows a continuous change from dark to bright under the crossed polars within 15 min. No obvious change can be observed when the system is exposed to one or two base-pair mismatch DNA targets. This system provides a principle for label-free and real-time detection of DNA targets without any fluorescent labeling.


Related Articles

  • Spectral and luminescence properties of gadolinium gallium garnet epitaxial films doped with terbium. Vasil’eva, N. V.; Randoshkin, V. V.; Kolobanov, V. N.; Kryukova, E. B.; Mikhaĭlin, V. V.; Petrovnin, N. N.; Plotnichenko, V. G.; Pyrkov, Yu. N.; Spasskiĭ, D. A.; Sysoev, N. N. // Physics of the Solid State;Mar2007, Vol. 49 Issue 3, p478 

    Gadolinium gallium garnet single-crystal films containing terbium are grown through liquid-phase epitaxy from a supercooled solution melt in the PbO-B2O3 system. The optical absorption spectra in the wavelength range 0.2–10.0 μm and the luminescence spectra excited by synchrotron...

  • Synthesis of [3H]-higenamine. Dezhu Wu; Zheng Zhang; Baoling Chen; Ginfu Li // Journal of Radioanalytical & Nuclear Chemistry;Nov2003, Vol. 258 Issue 3, p689 

    The synthesis of [3H]-higenamine by a four steps reaction, namely condensation, cyclization, catalytic tritiation and demethoxylation is described. The acidulated product was purified by thin layer chromatography and the final component has a high specific activity and radiochemical purity.

  • The formation of broken subboundaries in the elastic field of a planar mesodefect. Sarafanov, G. F.; Perevezentsev, V. N. // Technical Physics Letters;Apr2009, Vol. 35 Issue 4, p302 

    Based on an energy analysis within the framework of the disclination model, it is shown that a planar mesodefect can (similarly to the dipole of partial disclinations) initiate the formation of a band of reorientation. The process of band formation has been studied by means of computer...

  • Purification and biochemical characterization of an acidophilic amylase from a newly isolated Bacillus sp. DR90. Asoodeh, Ahmad; Alemi, Ashraf; Heydari, Akbar; Akbari, Jafar // Extremophiles;Mar2013, Vol. 17 Issue 2, p339 

    An acidophilic and Ca-independent amylase was purified from a newly isolated Bacillus sp. DR90 by ion-exchange chromatography, and exhibited a molecular weight of 68.9 kDa by SDS-PAGE. The optimum pH and temperature of the enzyme were found to be 4.0 and 45 °C, respectively. The enzyme...

  • The New TLC Method for Separation and Determination of Multicomponent Mixtures of Plant Extracts. Matysik, Elżbieta; Woźniak, Anna; Paduch, Roman; Rejdak, Robert; Polak, Beata; Donica, Helena // Journal of Analytical Methods in Chemistry;2/14/2016, p1 

    The new mode of two-dimensional gradient thin layer chromatography (MGD-2D TLC) has been presented. Short distance development of sample in the first dimension leads to formation of the preconcentrated narrow zones. They are consecutively separated in the second dimension with the mobile phase...

  • Separation and identification study of different metal ions by thin layer chromatography using mixture of urea - formaldehyde polymer and silica gel-G as thin layer and surfactants, organic solvents as mobile phase. Nagpurkar, Vijay S.; Deshmukh, Lata; Paliwal, L. J. // Der Chemica Sinica;2012, Vol. 3 Issue 4, p824 

    Different metal ions were separated on thin layer of laboratory prepared urea-formaldehyde polymer using cationic and anionic surfactants, organic , acidic and neutral mobile phases. Using different organic solvents in different ratios of water as a mobile phase different metal ions and their...

  • Micelle Enhanced Fluorimetric and Thin Layer Chromatography Densitometric Methods for the Determination of (�) Citalopram and its S -- Enantiomer Escitalopram. Taha, Elham A.; Salama, Nahla N.; Shudong Wang // Analytical Chemistry Insights;2009, Issue 4, p1 

    Two sensitive and validated methods were developed for determination of a racemic mixture citalopram and its enantiomer S-(+) escitalopram. The first method was based on direct measurement of the intrinsic fluorescence of escitalopram using sodium dodecyl sulfate as micelle enhancer. This was...

  • HPLC-FLUORESCENCE DETECTION. Robinson, Simon // Lab Manager Magazine;Mar2012, Vol. 7 Issue 2, p40 

    The article discusses the benefits of the high-performance thin-layer chromatography (HPTLC)-flourescence detection method for many components across various industries. It mentions that the detectors used for such method can ensure stable analysis and high levels of sensitivity to support...

  • Pharmacognostic and biomarker based Thin Layer Chromatographic profile of leaves of Nyctanthes arbor tris-tis L. Dutta, Sreya; Mallick, Buddhimanta; Mondal, Dhirendra Nath; Hazra, Kalyan; Majumdar, Chandan; Hazra, Jayram // Journal of Pharmacy Research;2012, Vol. 5 Issue 7, p3567 

    From ancient era, the leaf of 'Parijata' i. e. Nyctanthes arbor tris-tis L. has been highlighted in traditional system for its medicinal use. In present scenario the quality, purity and authenticity of a plant drug become questionable in herbal research. The relevant answer to these questions is...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics