Comparison study between direct and indirect labeling of estradiol for radioimmunoassay purpose

Sallam, Kh. M.; Mehany, N. L.
September 2009
Journal of Radioanalytical & Nuclear Chemistry;Sep2009, Vol. 281 Issue 3, p329
Academic Journal
In the present study, estradiol (E2) which contains an aromatic phenol ring in its chemical structure was chosen for direct and indirect labeling techniques using 125I and chloramine-T oxidation method. In direct technique the hydrogen atom in phenol ring was replaced by a radioiodine atom directly, which in indirect one, histidine methyl ester (HME) was labeled firstly with 125I using chloramine-T method then conjugated with E2. The 125I-labeled materials were purified using HPLC technique. The comparison study between the two obtained tracers was carried out in terms of radiochemical purity, binding percent, specific activity, non specific binding, binding displacement, shelf life using a radioimmunoassay (RIA) with specific antibody. The results indicated that indirect iodination of estradiol will cause significant increasing in studied parameters when combined with specific antibody than direct one.


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