TITLE

Quantification of Changes in c-myc mRNA Levels in Normal Human Bronchial Epithelial (NHBE) and Lung Adenocarcinoma (A549) Cells following Chemical Treatment

AUTHOR(S)
Fields, Wanda R.; Desiderio, Joseph G.; Putnam, Kathy P.; Bombick, David W.; Doolittle, David J.
PUB. DATE
September 2001
SOURCE
Toxicological Sciences;Sep2001, Vol. 63 Issue 1, p107
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Lung tumors frequently exhibit altered expression of oncogenes and/or tumor suppressor genes. Although some of these alterations are believed to arise from chemical exposure, the ability of specific chemicals to cause distinct changes in gene expression is not well characterized. We previously reported the development of a quantitative reverse transcriptase/polymerase chain reaction (RT/PCR) method for measuring c-myc mRNA levels, and reported that c-myc proto-oncogene expression is significantly increased in small-cell lung carcinoma cells. In the present study, quantitative RT/PCR was used to assess the effect of model toxins cycloheximide (CHX), a protein synthesis inhibitor, and N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), a DNA alkylating agent, on c-myc mRNA levels in normal human bronchial epithelial (NHBE) and lung adenocarcinoma (A549) cells. Expression of c-myc was evaluated at 1–100 μM CHX and MNNG and was compared to the cytotoxic response as measured by the neutral red assay. Cycloheximide elicited a dose-dependent increase in c-myc mRNA levels in NHBE and A549 cells, but did not alter expression of the housekeeping gene β-actin. A maximum increase for c-myc expression (200% of control) was observed 5 h after treatment with noncytotoxic concentrations. In contrast, MNNG elicited a dose-dependent decrease in c-myc expression in A549 cells, but no significant change in c-myc was observed in NHBE cells. The results from this study suggest that the quantitative RT/PCR method may be an appropriate technique for monitoring gene expression changes following chemical exposure. Hence, these types of studies may assist in the identification of specific chemicals which may induce the genetic alterations involved in the development of lung cancer as well as provide information relevant to the interactive effects of chemicals within complex mixtures.
ACCESSION #
44406195

 

Related Articles

  • Downregulation of miR-486-5p contributes to tumor progression and metastasis by targeting protumorigenic ARHGAP5 in lung cancer. Wang, J; Tian, X; Han, R; Zhang, X; Wang, X; Shen, H; Xue, L; Liu, Y; Yan, X; Shen, J; Mannoor, K; Deepak, J; Donahue, J M; Stass, S A; Xing, L; Jiang, F // Oncogene;2/27/2014, Vol. 33 Issue 9, p1181 

    We have previously shown that miR-486-5p is one of the most downregulated micro RNAs in lung cancer. The objective of the study was to investigate the role of miR-486-5p in the progression and metastasis of non-small-cell lung cancer (NSCLC). We evaluated miR-486-5p expression status on 76...

  • miR-126 Functions as a Tumor Suppressor in Osteosarcoma by Targeting Sox2. Chenglin Yang; Chunying Hou; Hepeng Zhang; Dewei Wang; Yan Ma; Yunqi Zhang; Xiaoyan Xu; Zhenggang Bi; Shuo Geng // International Journal of Molecular Sciences;Jan2014, Vol. 15 Issue 1, p423 

    Osteosarcoma (OS) is the most common malignant bone tumor in children and young adults, the early symptoms and signs of which are non-specific. The discovery of microRNAs (miRNAs) provides a new avenue for the early diagnosis and treatment of OS. miR-126 has been reported to be highly expressed...

  • MicroRNA-125b Functions as a Tumor Suppressor in Hepatocellular Carcinoma Cells. Hong-Yan Jia; Yu-Xuan Wang; Wen-Ting Yan; Hui-Yu Li; Yan-Zhang Tian; Shi-Ming Wang; Hao-Liang Zhao // International Journal of Molecular Sciences;Jul2012, Vol. 13 Issue 7, p8762 

    MicroRNAs (miRNAs) are important regulators of multiple cellular processes, and the deregulation of miRNA is a common event in diverse human diseases, particularly cancer. However, the mechanisms underlying the relationship between disordered miRNA expression and tumorigenesis have remained...

  • Distinct microRNA Expression Profile in Prostate Cancer Patients with Early Clinical Failure and the Impact of let-7 as Prognostic Marker in High-Risk Prostate Cancer. Schubert, Maria; Spahn, Martin; Kneitz, Susanne; Scholz, Claus Jürgen; Joniau, Steven; Stroebel, Philipp; Riedmiller, Hubertus; Kneitz, Burkhard // PLoS ONE;Jun2013, Vol. 8 Issue 6, p1 

    Background: The identification of additional prognostic markers to improve risk stratification and to avoid overtreatment is one of the most urgent clinical needs in prostate cancer (PCa). MicroRNAs, being important regulators of gene expression, are promising biomarkers in various cancer...

  • High-frequency promoter hypermethylation of the deleted in liver cancer-1 gene in multiple myeloma. Y-F Song // Journal of Clinical Pathology;Sep2006, Vol. 59 Issue 9, p947 

    BACKGROUND: Deleted in liver cancer-1 (DLC-1) is a tumour suppressor gene that is inactive in liver carcinogenesis. It encodes a ρ-guanosine triphosphatase-activating protein (ρ-GAP) and maps to one of the deleted regions (8p21.3–22). Little is known, however, about the methylation...

  • Expression of the Golgi phosphoprotein-3 gene in human gliomas: a pilot study. Li, Xue-Yuan; Liu, Wei; Chen, Shuang-Feng; Zhang, Lian-Qun; Li, Xin-Gang; Wang, Le-Xin // Journal of Neuro-Oncology;Nov2011, Vol. 105 Issue 2, p159 

    The oncogene Golgi phosphoprotein 3 (GOLPH3) has been found in several solid cancers, but its expression in glioma tumor tissues is unknown. Reverse transcription polymerase chain reaction and Western blot was used to investigate expression of GOLPH3 mRNA and protein, respectively, in 76...

  • Effects of human papillomavirus (HPV) type 16 oncoproteins on the expression of involucrin in human keratinocytes.  // Virology Journal;2012, Vol. 9 Issue 1, p36 

    The article discusses a study which aimed to analyze the effects of human papillomavirus (HPV) 16 E6 and E7 oncogenes on the expression of involucrin (IVL), an established marker of keratinocyte differentiation, in human foreskin keratinocyte (HFK) cells. The study found that the differentiation...

  • MicroRNA-92a Functions as an Oncogene in Colorectal Cancer by Targeting PTEN. Zhang, Guangjun; Zhou, He; Xiao, Huaxu; Liu, Zuoliang; Tian, Hongpeng; Zhou, Tong // Digestive Diseases & Sciences;Jan2014, Vol. 59 Issue 1, p98 

    Background: Our previous studies show that microRNA-92a (miR-92a) is overexpressed in colorectal cancer (CRC) and is thought to be correlated with the development of the cancer. However, its biological role in CRC remains poorly understood. Aims: The aim of the study was to determine the role of...

  • miR-21 and 221 upregulation and miR-181b downregulation in human grade II—IV astrocytic tumors. Alfredo Conti; M’Hammed Aguennouz; Domenico La Torre; Chiara Tomasello; Salvatore Cardali; Filippo Angileri; Francesca Maio; Annamaria Cama; Antonino Germanò; Giuseppe Vita; Francesco Tomasello // Journal of Neuro-Oncology;Jul2009, Vol. 93 Issue 3, p325 

    Abstract  MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. Recent evidence indicates that miRNAs can function both as tumor suppressors and as oncogenes. It has been demonstrated that...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics