Multispot point spread function for multiphoton fluorescence microscopy

Mondal, Partha Pratim
September 2009
Review of Scientific Instruments;Sep2009, Vol. 80 Issue 9, p096104
Academic Journal
We propose and demonstrate an imaging technique capable of generating multiple excitation spot for multiphoton fluorescence microscopy. The point spread function (PSF) is generated by interfering two counterpropagating extended depth of focus beams along the optical axis. At an illumination wavelength of 976 nm and aperture angle of 60°, five distinct nanospots of dimension ≈210 nm is obtained along the optical axis. The resulting PSF has the ability to simultaneously excite multiple planes, and overcomes the sidelobe problem associated with single-photon variant. The proposed multiple-excitation-spot-based-optical imaging technique may find potential application in nanobioimaging and three-dimensional fluorescence microscopy.


Related Articles

  • Understanding fluorescence blinking is the first path to an imaging solution. Vietmeyer, Felix; Volkan-Kacso, Sandor; Frantsuzov, Pavel; Kuno, Masaru; Janko, Boldizsar // Laser Focus World;Feb2011, Vol. 47 Issue 2, p45 

    The article focuses on the significance of fluorescence blinking in quantum emitters in the design of novel structures that show a substantial suppression of this blinking for improved imaging capabilities. The use of fluorescence techniques has spread rapidly and is a versatile tool in a wide...

  • Bioimaging: Cellular vision. Jenkins, Amber // Nature Photonics;Aug2008, Vol. 2 Issue 8, p464 

    The article cites key research findings that demonstrated how to overcome the resolution limit of fluorescence light microscopy in bioimaging using the technique called three-dimensional structured-illumination microscopy (3D-SIM). It includes an in-depth analysis of how the diffraction limit is...

  • Publisher's Note: "Limited-view light sheet fluorescence microscopy for three dimensional volume imaging" [Appl. Phys. Lett. 107, 263701 (2015)].  // Applied Physics Letters;2016, Vol. 108 Issue 4, p1 

    A correction to the article "Limited-view light sheet fluorescence microscopy for three dimensional volume imaging," that appeared in the 2015 issue is presented.

  • Beyond the rainbow: new fluorescent proteins brighten the infrared scene. Lin, Michael Z. // Nature Methods;Sep2011, Vol. 8 Issue 9, p726 

    In this article the author comments on the two studies involving the possibilities presented by red and infrared fluorescent proteins for mammalian optical imaging. The author states that photoactivation characteristics of PSmOrange could be beneficial for performing photoconversion in a large...

  • Invited Review Article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy. Carriles, Ramón; Schafer, Dawn N.; Sheetz, Kraig E.; Field, Jeffrey J.; Cisek, Richard; Barzda, Virginijus; Sylvester, Anne W.; Squier, Jeffrey A. // Review of Scientific Instruments;Aug2009, Vol. 80 Issue 8, p081101 

    We review the current state of multiphoton microscopy. In particular, the requirements and limitations associated with high-speed multiphoton imaging are considered. A description of the different scanning technologies such as line scan, multifoci approaches, multidepth microscopy, and novel...

  • Focus on fluorescence imaging. Evanko, Daniel // Nature Methods;Dec2005, Vol. 2 Issue 12, p901 

    The article discusses the use of fluorescence microscopy as a tool for imaging biological systems. The creation of fluorescent molecules and the exploitation of fluorescent proteins have resulted in the explosion in fluorescence microscopy techniques. However, the application of fluorescence...

  • Nonlinear Optical Characterization of Carbazole-derived Chromophores for Combined Second-harmonic and Two-photon Fluorescence Imaging. VAN CLEUVENBERGEN, STIJN; CLAYS, KOEN; DE MEULENAERE, EVELIEN; VANDERLEYDEN, JOS; WEI-QIANG CHEN; MEI-LING ZHENG; XUAN-MING DUAN; PSILODIMITRAKOPOULOS, SOTIRIS; LOZA-ALVAREZ, PABLO // Nonlinear Optics, Quantum Optics: Concepts in Modern Optics;2012, Vol. 45 Issue 1/2, p15 

    Carbazole-derived chromophores with enhanced second- and third-order nonlinear optical properties were engineered and characterized for their nonlinear optical properties, for ultimate use in combined second-harmonic and two-photon fluorescence microscopy imaging. Electron accepting moieties...

  • Fluorescence imaging in the millisecond time range of membrane electropermeabilisation of single cells using a rapid ultra-low-light intensifying detection system. Gabriel, B.; Teissi�, Justin // European Biophysics Journal;1998, Vol. 27 Issue 3, p291 

    A fast and sensitive fluorescence image acquisition system is described which uses an ultra-low-light intensifying camera able to acquire digitised fluorescence images with a time resolution of 3.33 ms/image. Two modes of recording were employed. The synchronisation mode allowed acquisition of...

  • Seeing fluorescence at super-resolution. Evanko, Daniel // Nature Methods;Jan2008, Vol. 5 Issue 1, p22 

    The article reports on the transformation of fluorescence microscopy by methods to improve its resolving power. The methods for super-resolution fluorescence imaging are described, which only require a commonly available microscope, specialized but easily obtainable fluorescent labels,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics