TITLE

Proteomics strategy for quantitative protein interaction profiling in cell extracts

AUTHOR(S)
Sharma, Kirti; Weber, Christoph; Bairlein, Michaela; Greff, Zoltán; Kéri, György; Cox, Jürgen; Olsen, Jesper V.; Daub, Henrik
PUB. DATE
October 2009
SOURCE
Nature Methods;Oct2009, Vol. 6 Issue 10, p741
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We report a proteomics strategy to both identify and quantify cellular target protein interactions with externally introduced ligands. We determined dissociation constants for target proteins interacting with the ligand of interest by combining quantitative mass spectrometry with a defined set of affinity purification experiments. We demonstrate the general utility of this methodology in interaction studies involving small-molecule kinase inhibitors, a tyrosine-phosphorylated peptide and an antibody as affinity ligands.
ACCESSION #
44373826

 

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