Electrical mapping of microtubular structures by surface potential microscopy

Peng Zhang; Cantiello, Horacio F.
September 2009
Applied Physics Letters;9/14/2009, Vol. 95 Issue 11, p113703
Academic Journal
Microtubules (MTs) are important cytoskeletal polymers that play an essential role in cell division and transport in all eukaryotes and information processing in neurons. MTs are highly charged polyelectrolytes, composed of hollow cylindrical arrangements of αβ-tubulin dimers. To date, there is little information about electrical properties of MTs. Here, we deposited and dried MTs onto a gold-plated surface to image their topology by atomic force microscopy (AFM), and determined their electrical mapping with surface potential microscopy (SPM). We found a strong linear correlation between the magnitude of relative surface potential and MT parameters, including diameter and height. AFM images confirmed the cylindrical topology of microtubular structures, and the presence of topological discontinuities along their surface, which may contribute to their unique electrical properties.


Related Articles

  • TgICMAP1 Is a Novel Microtubule Binding Protein in Toxoplasma gondii. Heaslip, Aoife T.; Ems-McClung, Stephanie C.; Ke Hu // PLoS ONE;2009, Vol. 4 Issue 10, p1 

    The microtubule cytoskeleton provides essential structural support for all eukaryotic cells and can be assembled into various higher order structures that perform drastically different functions. Understanding how microtubule-containing assemblies are built in a spatially and temporally...

  • Bridging membrane and cytoskeleton dynamics in the secretory and endocytic pathways. Anitei, Mihaela; Hoflack, Bernard // Nature Cell Biology;Jan2012, Vol. 14 Issue 1, p11 

    Transport carriers regulate membrane flow between compartments of the secretory and endocytic pathways in eukaryotic cells. Carrier biogenesis is assisted by microtubules, actin filaments and their associated motors that link to membrane-associated coats, adaptors and accessory proteins. We...

  • Strasburger's legacy to mitosis and cytokinesis and its relevance for the Cell Theory. Baluška, František; Volkmann, Dieter; Menzel, Diedrik; Barlow, Peter // Protoplasma;Oct2012, Vol. 249 Issue 4, p1151 

    Eduard Strasburger was one of the most prominent biologists contributing to the development of the Cell Theory during the nineteenth century. His major contribution related to the characterization of mitosis and cytokinesis and especially to the discovery of the discrete stages of mitosis, which...

  • Phosphorylation of Sli15 by Ipl1 Is Important for Proper CPC Localization and Chromosome Stability in Saccharomyces cerevisiae. Makrantoni, Vasso; Corbishley, Stephen J.; Rachidi, Najma; Morrice, Nicholas A.; Robinson, David A.; Stark, Michael J. R. // PLoS ONE;Feb2014, Vol. 9 Issue 2, p1 

    The chromosomal passenger complex (CPC) is a key regulator of eukaryotic cell division, consisting of the protein kinase Aurora B/Ipl1 in association with its activator (INCENP/Sli15) and two additional proteins (Survivin/Bir1 and Borealin/Nbl1). Here we have identified multiple sites of CPC...

  • MPF Governs the Assembly and Contraction of Actomyosin Rings by Activating RhoA and MAPK during Chemical-Induced Cytokinesis of Goat Oocytes. Yan-Guang Wu; Ping Zhou; Guo-Cheng Lan; Da Gao; Qing Li; De-Li Wei; Hui-Li Wang; Jing-He Tan // PLoS ONE;2010, Vol. 5 Issue 9, p1 

    The interplay between maturation-promoting factor (MPF), mitogen-activated protein kinase (MAPK) and Rho GTPase during actin-myosin interactions has yet to be determined. The mechanism by which microtubule disrupters induce the formation of ooplasmic protrusion during chemical-assisted...

  • Mitotic impairment by doublecortin is diminished by doublecortin mutations found in patients. Couillard-Despres, Sebastien; Uyanik, Goekhan; Ploetz, Sonja; Karl, Claudia; Koch, Hartmut; Winkler, Juergen; Aigner, Ludwig // Neurogenetics;Jun2004, Vol. 5 Issue 2, p83 

    Mutations in doublecortin (DCX) affect the migration of neuronal precursor cells and cause subcortical band heterotopia and lissencephaly. DCX is known to bind and bundle microtubules; however, the impact of mutation on DCX function and its relation to the manifestation of DCX-associated...

  • Assembly dynamics of microtubules at molecular resolution. Kerssemakers, Jacob W. J.; Munteanu, E. Laura; Laan, Liedewij; Noetzel, Tim L.; Janson, Marcel E.; Dogterom, Marileen // Nature;8/10/2006, Vol. 442 Issue 7103, p709 

    Microtubules are highly dynamic protein polymers that form a crucial part of the cytoskeleton in all eukaryotic cells. Although microtubules are known to self-assemble from tubulin dimers, information on the assembly dynamics of microtubules has been limited, both in vitro and in vivo, to...

  • Specialisation of the Tropomyosin Composition of Actin Filaments Provides New Potential Targets for Chemotherapy. Stehn, Justine R.; Schevzov, Galina; O'Neill, Geraldine M.; Gunning, Peter W. // Current Cancer Drug Targets;May2006, Vol. 6 Issue 3, p245 

    The actin microfilament network is important in maintaining cell shape and function in eukaryotic cells. It has a multitude of roles in cellular processes such as cell adhesion, motility, cellular signalling, intracellular trafficking and cytokinesis. Alterations in the organisation of the...

  • Neuronal deletion of GSK3β increases microtubule speed in the growth cone and enhances axon regeneration via CRMP-2 and independently of MAP1B and CLASP2. Liz, Márcia A.; Mar, Fernando M.; Santos, Telma E.; Pimentel, Helena I.; Marques, Ana M.; Morgado, Marlene M.; Vieira, Sílvia; Sousa, Vera F.; Pemble, Hayley; Wittmann, Torsten; Sutherland, Calum; Woodgett, James R.; Sousa, Mónica M. // BMC Biology;2014, Vol. 12 Issue 1, p1 

    Background In the adult central nervous system, axonal regeneration is abortive. Regulators of microtubule dynamics have emerged as attractive targets to promote axonal growth following injury as microtubule organization is pivotal for growth cone formation. In this study, we used conditioned...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics