Human Umbilical Cord Blood-derived Stromal Cells Suppress Xenogeneic Immune Cell Response In Vitro

Lei Hao; Cheng Zhang; Xing-hua Chen; Zhongmin Zou; Xi Zhang; Peiyan Kong; Xue Liang; Lei Gao; Xian-gui Peng; Aihua Sun; Qing-yu Wang
August 2009
Croatian Medical Journal;Aug2009, Vol. 50 Issue 4, p351
Academic Journal
Aim To explore immunological properties of human umbilical cord blood-derived stromal cells (hUCBDSC) and their effect on xenogeneic immune cells in vitro. Methods Immunological phenotype of freshly isolated and cryopreserved hUCBDSCs was evaluated by flow cytometry. Xenogeneic splenic T-cells were stimulated by phytohemaglutinin A (PHA) or dendritic cells in the absence or presence of hUCBDSCs. T-cell proliferation was measured by cell counting kit-8 after 7-day incubation. The proportion of apoptotic cells and CD4+CD25+Foxp3+ regulatory T-cells (Tregs) was determined in T-cells activated by PHA in the absence or presence of hUCBDSCs by flow cytometry. Phenotype of dendritic cells, cultured alone or with hUCBDSCs, was analyzed by flow cytometry. Results Levels of immune molecule expression on freshly isolated hUCBDSCs were as follows: human leukocyte antigen-I (HLA-I) (84.1 ± 2.9%), HLA-II (1.6 ± 0.3%), CD80 (0.8 ± 0.1%), CD86 (0.8 ± 0.1%), CD40 (0.6 ± 0.1%), and CD40L (0.5 ± 0.1%), which was not influenced by cryopreservation. T-cell proliferation in the presence of hUCBDSCs was significantly lower than that of positive control. The coculture led to a 10-fold increase (from 1.2 ± 0.3% to 12.1 ± 1.4%, P < 0.001) in the proportion of CD4+CD25+Foxp3+ regulatory T-cells (Tregs) and a reversion of mature dendritic cells, as indicated by the down-regulation of major histocompatibility complex (MHC)-II molecule (49.3% vs 25.9%, P = 0.001), CD80 (47.2% vs 23.3%, P = 0.001), and CD86 (40.6% vs 25.1%, P = 0.002). When subjected to annexin V binding and propidium iodide uptake assay, the hUCBDSCs did not show the ability to induce apoptosis of xenogeneic T-cells. Conclusion These results demonstrate low immunogenicity and immunomodulation effect of the hUCBDSCs. Reversion of mature dendritic cells and increase in Treg proportion, but not cell apoptosis, can possibly contribute to the suppression of xenogeneic T-cell proliferation by the hUCBDSCs.


Related Articles

  • Modulation of HLA class I antigen and ICAM-2 on endothelial cells after in vitro infection with.... SHAHGASEMPOUR, SHAPOUR; WOODROFFE, SALWA B; GARNETT, HELEN M; Woodroffe // Immunology & Cell Biology;Jun1998, Vol. 76 Issue 3, p217 

    The aim of the present work is to investigate the expression of HLA class I antigen and intercellular adhesion molecule-2 (ICAM-2) on the surface of human umbilical cord endothelial cells (HUVEC) after infection with human cytomegalovirus (HCMV). The expression of HLA class I antigen and ICAM-2...

  • Cross-presentation of a CMV pp65 epitope by human dendritic cells using bee venom PLA2 as a membrane-binding vector Babon, Aurélie; Almunia, Christine; Boccaccio, Claire; Beaumelle, Bruno; Gelb, Michael H.; Ménez, André; Maillère, Bernard; Abastado, Jean-Pierre; Salcedo, Margarita; Gillet, Daniel // FEBS Letters;Mar2005, Vol. 579 Issue 7, p1658 

    Abstract: We have used bee venom phospholipase A2 as a vector to load human dendritic cells ex vivo with a major histocompatibility complex (MHC) class I-restricted epitope fused to its C-terminus. The fusion protein bound to human monocyte-derived dendritic cells and was internalized into early...

  • HLA typing demands for peptide-based anti-cancer vaccine. Nagorsen, Dirk; Thiel, Eckhard // Cancer Immunology, Immunotherapy;Dec2008, Vol. 57 Issue 12, p1903 

    Immunological treatment of cancer has made some very promising advances during the last years. Anti-cancer vaccination using peptides or peptide-pulsed dendritic cells and adoptive transfer of in vitro generated, epitope-specific T cells depend on a well-fitting interaction of HLA molecule and...

  • Serpinb9 (Spi6)-deficient mice are impaired in dendritic cell-mediated antigen cross-presentation. Rizzitelli, Alexandra; Meuter, Simone; Vega Ramos, Javier; Bird, Catherina H; Mintern, Justine D; Mangan, Matthew SJ; Villadangos, Jose; Bird, Phillip I // Immunology & Cell Biology;Oct2012, Vol. 90 Issue 9, p841 

    Serpinb9 (Sb9, also called Spi6) is an intracellular inhibitor of granzyme B (GrB) that protects activated cytotoxic lymphocytes from apoptosis. We show here that the CD8+ subset of splenic dendritic cells (DC), specialized in major histocompatibility complex class I (MHC I) presentation of...

  • Cross-presentation of HCMV chimeric protein enables generation and measurement of polyclonal T cells. Nguyen, Thi H. O.; Sullivan, Lucy C.; Kotsimbos, Tom C.; Schwarer, Anthony P.; Mifsud, Nicole A. // Immunology & Cell Biology;Aug/Sep2010, Vol. 88 Issue 6, p676 

    CD8+ T cell immunity has a critical function in controlling human cytomegalovirus (HCMV) infection. In immunocompromized individuals, HCMV reactivation or disease can lead to increased morbidity and mortality, particularly in transplant recipients. In this setting, adoptive transfer of...

  • Immunosuppressive properties of human umbilical cord-derived mesenchymal stem cells: role of B7-H1 and IDO. Tipnis, Shabari; Viswanathan, Chandra; Majumdar, Anish S. // Immunology & Cell Biology;Nov/Dec2010, Vol. 88 Issue 8, p795 

    Umbilical cord is a rich source of mesenchymal stromal or stem cells (MSCs) that can be used for developing allogeneic cell therapy to treat intractable diseases. In this report, we present evidence that umbilical cord-derived MSCs (UCMSCs) possess important immunomodulatory properties that may...

  • Influence of Host Genetics on the Severity of Coccidioidomycosis. Louie, Leslie; Ng, Susanna // Emerging Infectious Diseases;Sep/Oct99, Vol. 5 Issue 5, p672 

    Coccidioidomycosis, a mild flulike illness in approximately 40% of infected persons, progresses to severe pulmonary or disseminated disease in 1% to 10% of symptomatic cases. We examined host genetic influences on disease severity among class II HLA loci and the ABO blood group. Participants...

  • HLA affinities of Iyers, a Brahmin population of Tamil Nadu, South India. Balakrishnan, K.; Pitchappan, R.M. // Human Biology;Aug96, Vol. 68 Issue 4, p523 

    Studies the HLA antigens of Iyers, a Brahmin population of Tamil Nadu in South India. HLA typing through a two-stage microlymphocytotoxicity assay; Processing and freezing of plasma in an EDTA solution for complement studies; Comparison of the Iyers with other populations.

  • Toxicity of interferon. Scott, G.M.; Secher, D.S.; Flowers, D.; Bate, J.; Cantell, K.; Tyrrell, D.A.J. // British Medical Journal (Clinical Research Edition);4/25/1981, Vol. 282 Issue 6273, p1345 

    Examines the effects of highly purified human leucocyte interferon on the human body. Conduction of monoclonal antibody affinity chromatography; Increase in pulse rate and body temperature; Similarity of local inflammatory reactions.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics