TITLE

Differential effects of ovarian steroids and raloxifene on serotonin 1A and 2C receptor protein expression in macaques

AUTHOR(S)
J. Henderson
PUB. DATE
June 2008
SOURCE
Endocrine (1355008X);Jun2008, Vol. 33 Issue 3, p285
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract  To further understand the role of ovarian hormones in the function of the serotonin neural system, we investigated the effects of estradiol (E), progesterone (P), and raloxifene on 5HT 1A and 2C receptor protein expression in the dorsal raphe region using Western blot analysis. Adult rhesus macaques (Macaca mulatta) were ovariectomized (Ovx) and implanted with Silastic capsules containing E or P. In the first paradigm, animals that had been Ovx for 6–16 months were treated for 1 month with E (E1) or E + P (EP1) and compared to animals that were untreated and Ovx for 5 months (n = 4 per group). In the second paradigm, comparisons were made between animals that were Ovx and untreated for 5 months, or Ovx and immediately implanted with Silastic capsules containing E or E + P for 5 months (E5, EP5), or administered raloxifene in the diet for 5 months (Ral5) (n = 4 per group). The dorsal raphe region was harvested, homogenized and a crude membrane fraction was obtained for examination of receptor proteins. In the first paradigm, 5HT1A receptor protein expression was significantly lower in E1 and EP1 treatment groups compared to the Ovx-control group (ANOVA P = 0.01; posthoc P P = 0.04; posthoc P P = 0.001; posthoc P P 
ACCESSION #
44228791

 

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