Interleukin-6 stimulates lipolysis in porcine adipocytes

Dapeng Ju; Mingtao Zhang; Gongshe Yang
June 2008
Endocrine (1355008X);Jun2008, Vol. 33 Issue 3, p261
Academic Journal
Abstract  Interleukin (IL)-6 stimulates lipolysis in human and rodents adipocytes. However, the mechanism regulating this process is little known. In this study, we demonstrated that IL-6 increased lipolysis in differentiated porcine adipocytes by activation of extracellular signal-related kinase (ERK), which was inhibited by specific ERK inhibitor PD98059. IL-6 treatment did not elevate intracellular cAMP and specific PKA inhibitor H89 did not affect IL-6-induced lipolysis, which suggested that protein kinase A (PKA) pathway was not involved in IL-6-induced lipolysis. Also, the expressions of perilipin A and PPARγ2 were significantly reduced in response to IL-6 treatment, but the expressions of peroxisome proliferators-activated receptor gamma coactivator-1 alpha (PGC-1α), carnitinepalmitoyl-transferase-1 (CPT-1), and uncoupling protein 2 (UCP2) were significantly elevated. In conclusion, these results suggested that chronic high dose of IL-6 directly stimulated lipolysis in porcine adipocytes through activation of ERK, subsequently repressing perilipin A and promoting PGC-1α expression.


Related Articles

  • Activation of the ERK signaling pathway is involved in CD151-induced angiogenic effects on the formation of CD151-integrin complexes. Hou-juan ZUO; Jing-yang LIN; Zhao-yu LIU; Wei-feng LIU; Tao LIU; Jun YANG; Yu LIU; Dao-wen WANG; Zheng-xiang LIU // Acta Pharmacologica Sinica;Jul2010, Vol. 31 Issue 7, p805 

    AbstractAim:To assess the roles of extracellular signal-regulated kinase (ERK), p38, and CD151-integrin complexes on proliferation, migration, and tube formation activities of CD151-induced human umbilical vein endothelial cells (HUVECs).Methods:CD151, anti-CD151 and CD151-AAA mutant were...

  • Tpl2 Kinase Is Upregulated in Adipose Tissue in Obesity and May Mediate Interleukin-1β and Tumor Necrosis Factor-α Effects on Extracellular Signal-Regulated Kinase Activation and Lipolysis. Jager, Jennifer; Grémeaux, Thierry; Gonzalez, Teresa; Bonnafous, Stéphanie; Debard, Cyrille; Laville, Martine; Vidal, Hubert; Tran, Albert; Gual, Philippe; Le Marchand-Brustel, Yannick; Cormont, Mireille; Tanti, Jean-François // Diabetes;Jan2010, Vol. 59 Issue 1, p61 

    OBJECTIVE--Activation of extracellular signal-regulated kinase-(ERK)-1/2 by cytokines in adipocytes is involved in the alterations of adipose tissue functions participating in insulin resistance. This study alms at identifying proteins regulating ERK1/2 activity, specifically in response to...

  • Stretch activation of Jun N-terminal kinase/stress-activated protein kinase in mesangial cells. Ingram, Alistair J.; James, Leightonh; Hao Ly; Thai, Kerri; Scholey, James W. // Kidney International;Oct2000, Vol. 58 Issue 4, p1431 

    Examines the activation of terminal kinase activated protein kinase in mesangial cells (MC). Production of extracellular matrix protein; Increase in stress-activated protein kinase due to cyclic strain/relaxation; Effects of mechanical forces on MC in vitro.

  • PI3-K/Akt pathway contributes to IL-6-dependent growth of 7TD1 cells. JiYan Zhang; Yan Li; BeiFen Shen // Cancer Cell International;2003, Vol. 3, p1 

    Background: Recently, growing evidence suggests the involvement of PI 3-K/Akt in IL-6- dependent survival and proliferative responses in several types of cells. However, whether PI 3-K/ Akt plays the same role in IL-6-dependent growth of 7TD1 mouse-mouse B cell hybridoma is not known. Methods:...

  • IL-6 induces lipolysis and mitochondrial dysfunction, but does not affect insulin-mediated glucose transport in 3T3-L1 adipocytes. Ji, Chenbo; Chen, Xiaohui; Gao, Chunlin; Jiao, Liuhong; Wang, Jianguo; Xu, Guangfeng; Fu, Hailong; Guo, Xirong; Zhao, Yaping // Journal of Bioenergetics & Biomembranes;Aug2011, Vol. 43 Issue 4, p367 

    Interleukin-6 (IL-6) has emerged as an important cytokine involved in the regulation of metabolism. However, the role of IL-6 in the etiology of obesity and insulin resistance is not fully understood. Mitochondria are key organelles of energy metabolism, and there is growing evidence that...

  • Regulation of mesangial cell proliferation. Schöcklmann, Harald O.; Lang, Stefan; Sterzel, R. Bernd // Kidney International;Oct1999, Vol. 56 Issue 4, p1199 

    Regulation of mesangial cell proliferation. Regardless of the source of injury, an imbalance in the control of mesangial cell proliferation appears to play a direct role in the degree of progressive renal injury and glomerulosclerosis. Some of the regulatory mechanisms include specific soluble...

  • Extracellular matrix as a determinant of signaling responses in glomerular epithelial cells. Cybulsky, Andrey V. // Kidney International;Oct1999, Vol. 56 Issue 4, p1242 

    Extracellular matrix as a determinant of signaling responses in glomerular epithelial cells. Glomerular epithelial cell proliferation occurs in renal diseases such as membranous nephropathy and crescentic glomerulonephritis. To understand the growth factors responsible for the proliferative...

  • Different Associations of CD45 Isoforms with STAT3, PKC and ERK Regulate IL-6-Induced Proliferation in Myeloma. Zheng, Xu; Li, Allison S.; Zheng, Huanyu; Zhao, Dongmei; Guan, Dagang; Zou, Huawei // PLoS ONE;Mar2015, Vol. 10 Issue 3, p1 

    In response to interleukin 6 (IL-6) stimulation, both CD45RO and CD45RB, but not CD45RA, translocate to lipid rafts. However, the significance of this distinct translocation and the downstream signals in CD45 isoforms-participated IL-6 signal are not well understood. Using sucrose fractionation,...

  • Interleukin-6 Regulation of AMP-Activated Protein Kinase. Ruderman, Neff B.; Keller, Charlotte; Richard, Ann-Marie; Saha, Asish K.; Luo, Zhijun; Xiang, Xiaoqin; Giralt, Mercedes; Ritov, Vladimir B.; Menshikova, Elizabeth V.; Kelley, David E.; Hidalgo, Juan; Pedersen, Bente K.; Kelly, Meghan // Diabetes;Dec2006 Supplement 2, Vol. 55, pS48 

    Interleukin (IL)-6 is a pleiotropic hormone that has both proinflammatory and anti-inflammatory actions. AMP-activated protein kinase (AMPK) is a fuel-sensing enzyme that among its other actions responds to decreases in cellular energy state by enhancing processes that generate ATP and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics