TITLE

Automated microextraction sample preparation coupled on-line to FT-ICR-MS: application to desalting and concentration of river and marine dissolved organic matter

AUTHOR(S)
Morales-Cid, Gabriel; Gebefugi, Istvan; Kanawati, Basem; Harir, Mourad; Hertkorn, Norbert; Rosselló-Mora, Ramón; Schmitt-Kopplin, Philippe
PUB. DATE
October 2009
SOURCE
Analytical & Bioanalytical Chemistry;Oct2009, Vol. 395 Issue 3, p797
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Sample preparation procedures are in most cases sample- and time-consuming and commonly require the use of a large amount of solvents. Automation in this regard can optimize the minimal-needed injection volume and the solvent consumption will be efficiently reduced. A new fully automated sample desalting and pre-concentration technique employing microextraction by packed sorbents (MEPS) cartridges is implemented and coupled to an ion cyclotron resonance Fourier-transform mass spectrometer (ICR-FT/MS). The performance of non-target mass spectrometric analysis is compared for the automated versus off-line sample preparation for several samples of aqueous natural organic matter. This approach can be generalized for any metabolite profiling or metabolome analysis of biological materials but was optimized herein using a well characterized but highly complex organic mixture: a surface water and its well-characterized natural organic matter and a marine sample having a highly salt charge and enabling to validate the presented automatic system for salty samples. The analysis of Suwannee River water showed selective C18-MEPS enrichment of chemical signatures with average H/C and O/C elemental ratios and loss of both highly polar and highly aromatic structures from the original sample. Automated on-line application to marine samples showed desalting and different chemical signatures from surface to bottom water. Relative comparison of structural footprints with the C18-concentration/desalting procedure however enabled to demonstrate that the surface water film was more concentrated in surface-active components of natural (fatty acids) and anthropogenic origin (sulfur-containing surfactants). Overall, the relative standard deviation distribution in terms of peak intensity was improved by automating the proposed on-line method. [Figure not available: see fulltext.]
ACCESSION #
44217542

 

Related Articles

  • High performance detection of biomolecules using a high magnetic field electrospray ionization... Ziqiang Guan; Campbell, Victoria L.; Drader, Jared J.; Hendrickson, Christopher L.; Laude Jr., David A. // Review of Scientific Instruments;Sep95, Vol. 66 Issue 9, p4507 

    Describes an improved, high-performance version of the concentric vacuum chamber shown for forming ions at high pressure in a strong magnetic field and detecting them in an adjacent Fourier transform ion cyclotron resonance mass spectrometry trapped ion cell. Addition of a mechanical shutter to...

  • Development of a Fourier-transform ion cyclotron resonance mass spectrometer-ion mobility spectrometer. Bluhm, Brian K.; Gillig, Kent J.; Gilig, Kent J.; Russell, David H. // Review of Scientific Instruments;Nov2000, Vol. 71 Issue 11 

    In an effort to incorporate ion-molecule reaction chemistry with ion mobility measurements we designed and constructed a novel instrument that combines a Fourier-transform ion cyclotron resonance (ICR) mass spectrometer with an ion mobility drift cell and a time-of-flight mass spectrometer....

  • Debating Resolution and Mass Accuracy. Balogh, Michael P. // LC-GC Asia Pacific;Nov2004, Vol. 7 Issue 4, p16 

    Discusses several factors to consider in evaluating a mass spectrometer's capabilities in liquid chromatography systems. Resolution of the ion cyclotron resonance instrument introduced by Thermo; Definition of mass accuracy; Differentiation of a sulfide from aldehyde metabolites by mass accuracy.

  • Technical approaches in the mass spectrometric analysis of hydrogen and helium isotopes. Abramov, I. A.; D&'yanov, N. yu.; Kazakovskii, N. T. // Instruments & Experimental Techniques;Jan2010, Vol. 53 Issue 1, p115 

    A method of mass spectrometric analysis of gas mixtures of hydrogen and helium isotopes on low-resolution units is proposed. The method is based on the dependence of the ion current intensity on the partial pressure of the gas in the puffing system of the mass spectrometer. The appropriate...

  • Panelists Look to Diverse Methods to Slake a State's Thirst. Williams, Rochelle // Bond Buyer;9/17/2002, Vol. 341 Issue 31472, p5 

    Reports the impact of growing population to the water needs in San Francisco, California. Standard method of desalinization and conservation of water; Estimation of 300 million dollars projected cost; Agreement between farmers and state legislation to conserve water by preventing irrigation...

  • Australia : Desalination plant: $1 billion paid, zero water ordered.  // TendersInfo News;7/30/2014, p1 

    The article reports on the amount paid by the water consumers in Melbourne, Victoria for the desalination plant in Wonthaggi which has been wasted and unmanaged since its completion in December 2012.

  • A Mass Spectrometry Primer: Part 2. Balogh, Michael P. // LC-GC Europe;Feb2009, Vol. 22 Issue 2, p86 

    The article compares the operations of several mass spectrometers. In quadrupole mass spectrometer, radio frequency and constant direct current (DC) between four parallel rods act as a mass separator. Unlike the quadropole, ion traps and ion cyclotron (ICR) devices store ions in a...

  • “Lossless” compression of high resolution mass spectra of small molecules. Blanckenburg, Bo; Burgt, Yuri E. M.; Deelder, André M.; Palmblad, Magnus // Metabolomics;Sep2010, Vol. 6 Issue 3, p335 

    Fourier transform ion cyclotron resonance (FTICR) provides the highest resolving power of any commercially available mass spectrometer. This advantage is most significant for species of low mass-to-charge ratio ( m/ z), such as metabolites. Unfortunately, FTICR spectra contain a very large...

  • “Linearizing” an ion cyclotron resonance cell. Barlow, S. E.; Tinkle, Mark D. // Review of Scientific Instruments;Dec2002, Vol. 73 Issue 12, p4185 

    We describe an ion cyclotron resonance (ICR) mass spectrometer that we have built. The design of the instrument was guided in large measure by theoretical consideration; in particular we wished to investigate the effects of improved electrostatic linearity on ICR performance. We found, for...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics