Arbitrary multisite two-photon excitation in four dimensions

Daria, Vincent Ricardo; Stricker, Christian; Bowman, Richard; Redman, Stephen; Bachor, Hans-A.
August 2009
Applied Physics Letters;8/31/2009, Vol. 95 Issue 9, p093701
Academic Journal
We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage.


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