TITLE

Selective probing of mRNA expression levels within a living cell

AUTHOR(S)
Nawarathna, D.; Turan, T.; Wickramasinghe, H. Kumar
PUB. DATE
August 2009
SOURCE
Applied Physics Letters;8/24/2009, Vol. 95 Issue 8, p083117
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We report on a selective and nondestructive measurement of mRNA (messenger ribonucleic acid) expression levels within a living cell. We first modify an atomic force microscope tip to create a tapered nanoscale coaxial cable. Application of an ac (alternating potential) between the inner and outer electrodes of this cable creates a dielectrophoretic force attracting mRNA molecules toward the tip-end which is pretreated with gene specific primers. We selectively extracted and analyzed both high (∼2500) and extremely low (110) copy number mRNA from a living cell mRNA in less than 10 s.
ACCESSION #
43944849

 

Related Articles

  • Study of Some Bidentate Schiff Bases of Isatin as Corrosion Inhibitors for Mild Steel in Hydrochloric Acid Solution. Singh, Ashish Kumar; Quraishi, M. A. // International Journal of Electrochemical Science;2012, Vol. 7 Issue 4, p3222 

    The effect of Schiff base compounds, namely, ethylenediamine bis-isatin (EDBI), hexane 1,4-diamine bis-isatin (HDBI) and thiocarbohydrazide bis-isatin (TCBI) were investigated by gravimetric, potentiodynamic polarization, electrochemical impedance spectroscopy , atomic force microscopy and...

  • Nanotweezers consisting of carbon nanotubes operating in an atomic force microscope. Akita, Seiji; Nakayama, Yoshikazu; Mizooka, Syotaro; Takano, Yuichi; Okawa, Takashi; Miyatake, Yu; Yamanaka, Sigenori; Tsuji, Masashi; Nosaka, Toshikazu // Applied Physics Letters;9/10/2001, Vol. 79 Issue 11 

    We have developed nanotweezers consisting of carbon nanotubes that will operate in an atomic force microscope. The two nanotubes were attached on the metal electrodes patterned on a conventional Si tip and their fixations were made by carbon deposition. These processes were made under the view...

  • Force microscopy imaging in liquids using ac techniques. Lantz, M.A.; O'Shea, S.J.; Welland, M.E. // Applied Physics Letters;7/25/1994, Vol. 65 Issue 4, p409 

    Investigates atomic force microscopy for imaging in liquids using alternating current techniques. Discovery of less noise in cantilever oscillation method; Use of sample oscillation technique for noncontact imaging; Demonstration of noncontact magnetic force imaging under liquid.

  • Making Ripple Measurements. Sandler, Steve // Power Electronics Technology Exclusive Insight;9/5/2012, p6 

    The article focuses on the making of ripple of measurement. It mentions that oscilloscope is the traditional device used in making ripple measurements but it is also considered as not the best choice because of its poor sensitivity, low resolution, and poor signal-to-noise performance (SNR). It...

  • Route of glucocorticoid-induced macromolecules across the nuclear envelope as viewed by atomic force microscopy. Shahin, Victor // Pflugers Archiv European Journal of Physiology;Oct2006, Vol. 453 Issue 1, p1 

    Glucocorticoids are vital steroid hormones. The physiologic activities of these hydrophobic molecules predominantly require translocation of glucocorticoid-initiated macromolecules (GIMs), proteins and mRNA transcripts, in and out of the nucleus, respectively. The bidirectional transport of GIMs...

  • Localized sureface elasticity measurements using an atomic force microscope. DeVecchio, Derik; Bhushan, Bharat // Review of Scientific Instruments;Dec1997, Vol. 68 Issue 12, p4498 

    Investigates localized surface elasticity measurements using an atomic force microscope. Identification and mapping of the differences in the stiffness of elasticity of ultrathin films; Provision of an alternative to current elasticity measurements; Application of the technique to...

  • Eigenfrequencies of a rectangular atomic force microscope cantilever in a medium. Elmer, Franz-Josef; Dreier, Marjus // Journal of Applied Physics;6/15/1997, Vol. 81 Issue 12, p7709 

    Presents a study calculating the eigenfrequencies of a rectangular antilever of an atomic force microscope (AFM) immersed in fluid or gas. Solution presented to the problem of combined elastomechanical and hydrodynamical equations; Evidence presented verifying the phenomenological theory;...

  • Calibration of frictional forces in atomic force microscopy. Ogletree, D.F.; Carpick, R.W.; Salmeron, M. // Review of Scientific Instruments;Sep96, Vol. 67 Issue 9, p3298 

    Describes the calibration of frictional forces in atomic force microscopy. Presentation of an in situ experimental procedure to determine the response of a cantilever to lateral forces in terms of its normal force response; Discussion of issues related to force and friction measurements using...

  • Difference between the forces measured by an optical lever deflection and by an optical interferometer in an atomic force microscope. Fujisawa, Satoru; Ohta, Masahiro; Konishi, Takefumi; Sugawara, Yasuhiro; Morita, Seizo // Review of Scientific Instruments;Mar94, Vol. 65 Issue 3, p644 

    Using a simple model, we investigated the difference between the forces measured by an atomic force microscope (AFM) with an optical lever deflection method and that with an optical interferometer method. Then, using a mica with an atomically flat surface as a test sample, we confirmed...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics