Protein GB1 Folding and Assembly from Structural Elements

Bauer, Mikael C.; Wei-Feng Xue; Linse, Sara
April 2009
International Journal of Molecular Sciences;Apr2009, Vol. 10 Issue 4, p1552
Academic Journal
Folding of the Protein G B1 domain (PGB1) shifts with increasing salt concentration from a cooperative assembly of inherently unstructured subdomains to an assembly of partly pre-folded structures. The salt-dependence of pre-folding contributes to the stability minimum observed at physiological salt conditions. Our conclusions are based on a study in which the reconstitution of PGB1 from two fragments was studied as a function of salt concentrations and temperature using circular dichroism spectroscopy. Salt was found to induce an increase in β-hairpin structure for the C-terminal fragment (residues 41 - 56), whereas no major salt effect on structure was observed for the isolated N-terminal fragment (residues 1 - 41). In line with the increasing evidence on the interrelation between fragment complementation and stability of the corresponding intact protein, we also find that salt effects on reconstitution can be predicted from salt dependence of the stability of the intact protein. Our data show that our variant (which has the mutations T2Q, N8D, N37D and reconstitutes in a manner similar to the wild type) displays the lowest equilibrium association constant around physiological salt concentration, with higher affinity observed both at lower and higher salt concentration. This corroborates the salt effects on the stability towards denaturation of the intact protein, for which the stability at physiological salt is lower compared to both lower and higher salt concentrations. Hence we conclude that reconstitution reports on molecular factors that govern the native states of proteins.


Related Articles

  • A Measurement System For Circular Dichroism In Soft X-ray Absorption Using Helicity Switching By Twin Helical Undulators. Muro, T.; Saitoh, Y.; Kimura, H.; Matsushita, T.; Nakatani, T.; Takeuchi, M.; Hirono, T.; Kudo, T.; Nakamura, T.; Wakita, T.; Kkobayashi, K.; Hara, T.; Shirasawa, K.; Kitamura, H.; Warwick, T. // AIP Conference Proceedings;2004, Vol. 705 Issue 1, p1051 

    A measurement system for circular dichroism (CD) of soft X-ray absorption has been developed using helicity switching of circularly polarized radiation by twin helical undulators at BL25SU of SPring-8. Difference of horizontal spot positions between the negative- and positive-helicity light at...

  • Protein voltammetry and spectroscopy: integrating approaches. Male, Louise; Marritt, Sophie J.; Berks, Ben C.; Cheesman, Myles R.; van Wonderen, Jessica H.; George, Simon J.; Butt, Julea N. // Theoretical Chemistry Accounts: Theory, Computation, & Modeling;Jan2008, Vol. 119 Issue 1-3, p107 

    Cyclic voltammetry readily visualizes the redox properties of many proteins. Net electron exchange between the protein and an electrode produces an electrical current that simultaneously quantitates and characterizes the underlying redox event(s). However, no direct information regarding the...

  • Synthesis, Chiral Resolution, and Absolute Configuration of Functionalized Tr�ger's Base Derivatives: Part II. Benkh�user-Schunk, Christian; Wezisla, Boris; Urbahn, Kirstin; Kiehne, Ulf; Daniels, J�rg; Schnakenburg, Gregor; Neese, Frank; L�tzen, Arne // ChemPlusChem;May2012, Vol. 77 Issue 5, p396 

    Seven racemic derivatives of Tr�ger's base-the 1,7-dibromo-substituted derivative 3, the 2,8-dibromo-substituted derivative 4, the 2,8-diiodo-substituted derivative 5, the 3,9-diiodo-substituted derivative 6, the 4,10-dibromo-substituted derivative 7, its singly debrominated analogue 8, and...

  • Determination of Absolute Configuration.  // Spectroscopy;Mar2006 Supplement, Vol. 21, p19 

    The article shows an example of determination of absolute configuration (AC) for the polyphenolic binaphpthyl dialdehyde, gossypol. It discusses the ability of gossypol to inhibit maturation in human sperm and its potential as an antineoplastic agent. The article details the use of vibrational...

  • Reduction of linear birefringence in vibrational circular dichroism measurement: use of a rotating half-wave plate. Xiaolin Cao; Dukor, Rina K.; Nafie, Laurence A. // Theoretical Chemistry Accounts: Theory, Computation, & Modeling;Jan2008, Vol. 119 Issue 1-3, p69 

    A new method has been developed to suppress the effects of linear birefringence (LB) associated with sample cells in the measurement of vibrational circular dichroism (VCD) spectra by adding a rotating half-wave plate (RHWP) to the optical train. The method functions in combination with the...

  • Probing chiral solute-water hydrogen bonding networks by chirality transfer effects: A vibrational circular dichroism study of glycidol in water. Yang, Guochun; Xu, Yunjie // Journal of Chemical Physics;4/28/2009, Vol. 130 Issue 16, p164506 

    Vibrational absorption (VA) and vibrational circular dichroism (VCD) spectra of (S)-(-)-glycidol were measured in water with a concentration of 6.0M in the 1000–1750 cm-1 region. Prominent and complex VCD spectral features were detected at the water bending vibrational region. Our...

  • The external heavy atom effect: Theory of spin–orbit coupling of alkali and noble metals in rare gas matrices. Pellow, Robert; Vala, Martin // Journal of Chemical Physics;5/15/1989, Vol. 90 Issue 10, p5612 

    Recent data from magnetic circular dichroism (MCD) and optical absorption studies of alkali and noble metal atoms in rare gas matrices have shown an interesting and significant influence of the rare gas on the spin–orbit (SO) splitting of the metal excited 2P state. The excited state SO...

  • Dielectric tensor formulation of magnetic dichroism sum rules. Zhao, Dai; Hochst, Hartmut; Huber, David L. // Journal of Applied Physics;9/1/1998, Vol. 84 Issue 5, p2858 

    Describes the formalism used to interpret the magnetic circular dichroism sum rules in terms of the complex dielectric tensor. Utilization of the Maxwell-Fresnel theory; Application of the formalism applied to the reflection spectra of iron; Consequences of the energy dependence of the...

  • Erratum: Magnetic circular dichroism of Na3Eu(ODA)3·2NaClO4·6H2O [J. Chem. Phys. 100, 815 (1994)]. Görller-Walrand, C.; Verhoeven, P.; D’Olieslager, J.; Fluyt, L.; Binnemans, K. // Journal of Chemical Physics;10/15/1994, Vol. 101 Issue 8, p7189 

    Presents a correction on an article about magnetic circular dichroism.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics