TITLE

Highly parallel measurements of interaction kinetic constants with a microfabricated optomechanical device

AUTHOR(S)
Bates, Steven R.; Quake, Stephen R.
PUB. DATE
August 2009
SOURCE
Applied Physics Letters;8/17/2009, Vol. 95 Issue 7, p073705
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We used mechanical trapping of molecular interactions to demonstrate a highly parallel approach to measure the kinetics of biomolecular interactions. This approach consumes 25 fmol of material per measurement and permits 320 measurements in a single experiment. We measured association and dissociation curves for the interactions of 6-His and T7 epitope tags with their antibodies, from which we determined the off rates, on rates, and dissociation constants.
ACCESSION #
43887401

 

Related Articles

  • Characterization of Antibody-Binding Sites on Proteins: Development of a Knowledgebase and Its Applications in Improving Epitope Prediction. Ghate, A.D.; Bhagwat, B.U.; Bhosle, S.G.; Gadepalli, S.M.; Kulkarni-Kale, U.D. // Protein & Peptide Letters;Jun2007, Vol. 14 Issue 6, p531 

    Immunoinformatics provides tools for reverse vaccinology and encompasses development of knowledge bases and algorithms for prediction of epitopes. AgAbDb, a database archiving molecular interactions of antigen-antibody cocrystal structures, has been developed (http://202.41.70.51:8080/agabdb2/)....

  • Vibrational dynamics up to the dissociation threshold: A case study of two-dimensional HOCl. Joyeux, Marc; Sugny, Dominique; Lombardi, Maurice; Jost, Remy; Schinke, Reinhard; Skokov, Sergei; Bowman, Joe¨l // Journal of Chemical Physics;12/1/2000, Vol. 113 Issue 21 

    This work is aimed at extending recent studies dealing with the highly excited vibrational dynamics of HOCl [J. Chem. Phys. 111, 6807 (1999); J. Chem. Phys. 112, 77 (2000)], by taking advantage of the fact that the OH-stretch remains largely decoupled from the two other degrees of freedom up to...

  • N[sub 2] dissociative adsorption on Ru(0001): The role of energy loss. Diekho¨ner, L.; Mortensen, H.; Baurichter, A.; Jensen, E.; Petrunin, V. V.; Luntz, A. C. // Journal of Chemical Physics;11/15/2001, Vol. 115 Issue 19, p9028 

    New molecular beam experiments on the dissociation probability S[sub 0] for N[sub 2] on Ru(0001) are presented. These are in general agreement with prior measurements and exhibit very unusual behavior; a very slow increase of S[sub 0] with incident kinetic energy E and the fact that S[sub 0] is...

  • Nonequilibrium unimolecular dissociation influenced by intramolecular vibrational energy.... Kato, Toshiko // Journal of Chemical Physics;4/22/1998, Vol. 108 Issue 16, p6611 

    Formulates the dissociation rates of energized molecules influenced by the dynamics of slow intramolecular vibrational energy redistribution (IVR). Application of Rice-Ramsperger-Kassel-Marcus to various unimolecular dissociation reactions; Evolution of the steady-state condition due to IVR and...

  • Double-resonance overtone photofragment spectroscopy of trans-HONO. II. State- and time-resolved dissociation and OH-product state distributions. Reiche, F.; Abel, B.; Beck, R. D.; Rizzo, T. R. // Journal of Chemical Physics;6/15/2002, Vol. 116 Issue 23, p10267 

    Linewidths, unimolecular dissociation rates and product state distributions (PSDs) have been measured for single rovibratational states of the v[sub 1]= 5-7 levels of gas-phase trans-nitrous acid (HONO) by double-resonance overtone photofragment spectroscopy (DROPS). The linewidth measurements,...

  • Spectroscopy and predissociation dynamics of the A[sup 1]A'' state of HNO. Pearson, Jonathan; Orr-Ewing, Andrew J.; Ashford, Michael N.R.; Dixon, Richard N. // Journal of Chemical Physics;4/8/1997, Vol. 106 Issue 14, p5850 

    Investigates the spectroscopy and predissociation dynamics of the A[sub 2]A electronic state of HNO by measurement of line positions and lifetime broadened linewidth in the cavity ring-down spectrum. Lorentzian components of line shapes of rovibrational features; Line broadening on transitions...

  • Photodissociation of F2 and mobility of F atoms in crystalline argon. Feld, J.; Kunttu, H.; Apkarian, V. A. // Journal of Chemical Physics;7/15/1990, Vol. 93 Issue 2, p1009 

    Experimental studies on the photodissociation of F2 in 12 K crystalline argon solids are reported, and compared with molecular dynamics (MD) simulations of the same system. At excess energies above 2.5 eV,the dissociation probability is near unity, in agreement with theory. At the longest...

  • Complicated and dissociative dynamics for the weakly forced and weakly damped Morse oscillator. Permann, Delmar; Hamilton, Ian // Journal of Chemical Physics;12/1/1991, Vol. 95 Issue 11, p8188 

    We examine the dynamics of a Morse oscillator which is weakly forced and weakly damped with initial conditions close to the dissociation energy. We calculate higher derivatives of the phase of the Morse oscillator with respect to time and use these to construct higher-order phase plots. We use...

  • Prediction of a neutralizing epitope of a H5N1 virus hemagglutinin complexed with an antibody variable fragment using molecular dynamics simulation. Mulyanto, Cenmidtal C.; Saleh, Rosari // Journal of Biophysical Chemistry;Aug2011, Vol. 2 Issue 3, p258 

    In this present study, we predicted the neutral- izing epitope of a modeled H5N1 hemagglutinin 1046T when interacted with a modeled mono- clonal antibody variable fragment 8H5Fv using molecular dynamics simulation. Following the production run of the molecular dynamics simu- lation, we observed...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics