T Cell Memory Response to Pneumococcal Protein Antigens in an Area of High Pneumococcal Carriage and Disease

Mureithi, Marianne W.; Finn, Adam; Ota, Martin O.; Qibo Zhang; Davenport, Victoria; Mitchell, Timothy J.; Williams, Neil A.; Adegbola, Richard A.; Heyderman, Robert S.
September 2009
Journal of Infectious Diseases;9/1/2009, Vol. 200 Issue 5, p783
Academic Journal
Background. Streptococcus pneumoniae is a leading cause of vaccine-preventable disease worldwide. Pneumococcal protein antigens are currently under study as components of potential vaccines that offer protection against multiple serotypes. We have therefore characterized T cell pneumococcal immunity acquired through asymptomatic carriage. Methods. Peripheral blood mononuclear cells from 40 healthy Gambian adults were stimulated with supernatants derived from S. pneumoniae strain (D39), 2 isogenic mutant strains lacking either pneumolysin or choline binding protein A, and recombinant pneumolysin. Immune responses were measured by cellular proliferation and by interleukin-10 (IL-10) and interferon-γ (IFN-γ) enzyme-linked immunosorbent spot and bioplex cytokine assays. Nasopharyngeal swabs were cultured to determine carriage rates. Results. S. pneumoniae nasopharyngeal carriage was detected in 60% of individuals. Both effector and resting (or central) CD4+ T cell memory were frequently present to a range of pneumococcal antigens. However, the level of the effector memory response did not relate to current nasopharyngeal carriage. Pneumolysin was not immunodominant in these T cell responses but induced a distinct proinflammatory profile (high IFN-γ, IL-12[p40], and L-17 levels and low IL-10 and IL-13 levels). Conclusions. In this population, T cell-mediated immunological memory potentially capable of pathogen clearance and immune surveillance is common but is not associated with the absolute interruption of pneumococcal carriage. How this naturally acquired immune memory influences pneumococcal vaccine efficacy remains to be determined.


Related Articles

  • Natural regulatory T cells and self-tolerance. Schwartz, Ronald H // Nature Immunology;Apr2005, Vol. 6 Issue 4, p327 

    The adaptive immune system allows individual organisms to mount defensive reactions against unanticipated pathogens by developmentally creating a diverse repertoire of clonally distributed receptors capable of recognizing a multitude of antigens and then expanding as effector cell populations...

  • An affinity/avidity model of peripheral T cell regulation. Hong Jiang; Yilun Wu; Bitao Liang; Zongyu Zheng; Guomei Tang; Kanellopoulos, Jean; Soloski, Mark; Winchester, Robert; Goldstein, Itamar; Chess, Leonard // Journal of Clinical Investigation;Feb2005, Vol. 115 Issue 2, p302 

    We show in these studies that Qa-1-dependent CD8+ T cells are involved in the establishment and maintenance of peripheral self tolerance as well as facilitating affinity maturation of CD4+ T cells responding to foreign antigen. We provide experimental evidence that the strategy used by the...

  • Lymphocyte Cell-Cycle Inhibition by HLA-G Is Mediated by Phosphatase SHP-2 and Acts on the mTOR Pathway. Ketroussi, Farah; Giuliani, Massimo; Bahri, Rajia; Azzarone, Bruno; Charpentier, Bernard; Durrbach, Antoine // PLoS ONE;2011, Vol. 6 Issue 8, p1 

    Human leukocyte antigen G (HLA-G) is involved in regulating T-cell responses through its interaction with inhibitory receptors belonging to the immunoglobulin-like transcript family (ILT). In this context, we investigated the pathways involved in the control of cell-cycle entry of T cells...

  • A Potential Role for CD1a Molecules on Human Epidermal Langerhans Cells in Allogeneic T-Cell Activation. Moulon, Corinne; Péguet-Navarro, Josette; Schmitt, Daniel // Journal of Investigative Dermatology;Sep91, Vol. 97 Issue 3, p524 

    The structural similarities of CD1a molecules to major histocompatibility complex (MHC) class I antigens, as well as their expression on epidermal antigen-presenting cells suggest that CD1a molecules might be involved in the cutaneous immune response. In the present study, we investigated the...

  • Absorption and presentation of antigens by epithelial cells of the small intestine: Hypotheses and predictions relating to the pathogenesis of coeliac disease. Mayrhofer, Graham // Immunology & Cell Biology;Oct1995, Vol. 73 Issue 5, p433 

    Effects of the route of antigen absorption and presentation in the small intestine are discussed in relation to outcomes from antigen exposure. It is proposed that antigens presented by epithelial cells to intrinsic T cell populations in the mucosa induce anti-inflammatory immune responses,...

  • Regulation of T cell function in mucosal tolerance. Hoyne, Gerard F.; Lamb, Jonathan R. // Immunology & Cell Biology;Apr1997, Vol. 75 Issue 2, p197 

    Administering antigens through mucosal surfaces leads to the induction of antigen-specific T cell unresponsiveness. This property of the mucosal immune system is now beginning to be exploited in the design of immunotherapeutic strategies aimed at targeting disease-inducing T cell populations....

  • Identification of Rat Respiratory Mucosa Stem Cells and Comparison of the Early Neural Differentiation Potential with the Bone Marrow Mesenchymal Stem Cells In Vitro. Gao, Xin; Zhang, Jian; Zhang, Jun; Zou, Hongjun; Liu, Jinbo // Cellular & Molecular Neurobiology;Mar2014, Vol. 34 Issue 2, p257 

    The aim of this study is to identify rat nasal septum respiratory mucosa-derived mesenchyme stem cells (RM-MSCs) and to compare its neural lineage differentiation capacity with bone marrow-derived mesenchyme stem cells (BM-MSCs) after a short period of neural induction culture in vitro. The cell...

  • Gr1+IL-4-producing innate cells are induced in response to Th2 stimuli and suppress Th1-dependent antibody responses. Amy S. McKee; Megan MacLeod; Janice White; Frances Crawford; John W. Kappler; Philippa Marrack // International Immunology;May2008, Vol. 20 Issue 5, p659 

    Alum is used as a vaccine adjuvant and induces Th2 responses and Th2-driven antibody isotype production against co-injected antigens. Alum also promotes the appearance in the spleen of Gr1+IL-4+ innate cells that, via IL-4 production, induce MHC II-mediated signaling in B cells. To investigate...

  • an·ti·bod·y.  // American Heritage Student Science Dictionary;2009, p17 

    A definition of the term "antibody" is presented. It is a protein produced in the blood or tissues in response to the presence of a specific foreign antigen. Antibodies provide immunity against certain microorganisms and toxins by binding with them and often by deactivating them. The immune...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics