TITLE

Induction of CC and CXC Chemokines in Human Antigen-Presenting Dendritic Cells by the Pneumococcal Proteins Pneumolysin and CbpA, and the Role Played by Toll-Like Receptor 4, NF-κB, and Mitogen-Activated Protein Kinases

AUTHOR(S)
Bernatoniene, Jolanta; Qibo Zhang; Dogan, Semih; Mitchell, Tim J.; Paton, James C.; Finn, Adam
PUB. DATE
December 2008
SOURCE
Journal of Infectious Diseases;12/15/2008, Vol. 198 Issue 12, p1823
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background. Choline-binding protein A (CbpA) and pneumolysin (Ply) can induce the expression and release of chemokines by human cells, which might modulate specific immune responses. In dendritic cells (DCs), such effects could be important for the size and character of the immunity induced if administered as vaccines. We studied the induction of CCL and CXCL chemokines by CbpA and Ply in DCs and related signaling pathways. Methods. Proteins derived from bacterial cultures and cloning were used as stimulants. DCs were generated from CD14+ human monocytes by negative selection, followed by coculture with recombinant human granulocytemacrophage colony-stimulating factor and recombinant interleukin-4. The role played by Toll-like receptors (TLRs) was assessed using anti-TLR antibodies. Likewise, specific inhibitors (given in parentheses) of signaling molecules were used: NF-κB (SN50), extracellular signal-regulated kinase (PD98059), p38 (SB203580), and Jun N-terminal kinase (SP600125). Results. Both CbpA and Ply significantly up-regulated DC mRNA of several CCL (2, 4, 5, and 8) and CXCL (8 and 10) chemokines studied as well as the expression of 3 proteins studied: CCL2, CCL5, and CXCL8. Ply stimulation was blocked by anti-TLR4. Inhibition of NF-κB and several mitogen-activated protein kinase signaling pathways also reduced chemokine release. Conclusion. Chemokine induction in DCs by CbpA and Ply may be important for their potential use in future pneumococcal vaccines.
ACCESSION #
43804681

 

Related Articles

  • Comparison of two different protocols for the induction of maturation of human dendritic cells in vitro. Colic, Miodrag; Mojsilovic, Slavko; Pavlovic, Bojan; Vucicevic, Dragana; Majstorovic, Ivana; Bufan, Biljana; Stojic-Vukanic, Zorica; Vasilijic, Sasa; Vucevic, Dragana; Gasic, Sonja; Balint, Bela // Vojnosanitetski Pregled: Military Medical & Pharmaceutical Journ;Sep/Oct2004, Vol. 61 Issue 5, p471 

    Background. Dendritic cells (DC) have been used for immunotherapy of malignant tumors, different kinds of infections, and other clinical conditions. For that purpose, optimal conditions for the generation of functionally mature DC in vitro are required. Two different protocols for the induction...

  • Unilineage monocytopoiesis in hematopoietic progenitor culture: switching cytokine treatment at all Mo developmental stages induces differentiation into dendritic cells. Montesoro, E.; Castelli, G.; Morsilli, O.; Nisini, R.; Stafsnes, M. H.; Carè, A.; Peschle, C.; Chelucci, C. // Cell Death & Differentiation;Feb2006, Vol. 13 Issue 2, p250 

    We have developed a new culture system whereby human hematopoietic progenitors purified from adult peripheral blood extensively proliferate and gradually differentiate into >95% pure monocytic (Mo) cells. At all developmental stages treatment with interleukin (IL)-4+granulocyte-macrophage...

  • Functional and Key Gene Expression Analyses of Chicken Monocyte-derived Dendritic Cells with Recombinant Interleukin 4. Khaje, Hamideh; Bahari, Abbas; Lagzian, Milad; Sabbagh, Seyed Kazem // Iranian Journal of Allergy, Asthma & Immunology;Dec2016, Vol. 15 Issue 6, p508 

    The central role of dendritic cells (DCs) as bridging innate and adaptive immunity leads to the expanding use of these cells in the poultry vaccine studies. The most effective way to produce enough DCs is monocyte transformation by combined induction of interleukin-4 (IL-4) and...

  • Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism. Ratthé, Claude; Ennaciri, Jamila; Garcês Gonçalves, David M.; Chiasson, Sonia; Girard, Denis // Mediators of Inflammation;2009, Vol. 2009, Special section p1 

    Interleukin (IL)-4 is a cytokine known mainly for its anti-inflammatory activity. Using the in vivo murine air pouch model, we found that IL-4 significantly increased the number of leukocytes after 9 hours of treatment, consisting mainly of neutrophil (60%) and monocytic (40%) cell populations....

  • Lymphotactin contransfection enhance the therapeutic efficacy of dendritic cells genetically modified with melanoma antigen gp100. Xia, D.J.; Zhang, W.P.; Zheng, S.; Wang, J.; Pan, J.P.; Wang, Q.; Zhang, L.H.; Hamada, H. // Gene Therapy;5/1/2002, Vol. 9 Issue 9, p592 

    Lymphotactin (Lptn) is a C chemokine that attracts T cells and NK cells. Dendritic cells (DC) are highly efficient, specialized-antigen-presenting cells and antigen-pulsed DC has been regarded as promising vaccines in cancer immunotherapy. The aim of our present study is to improve the...

  • Regulation of TH2 development by CXCR5+ dendritic cells and lymphotoxin-expressing B cells. León, Beatriz; Ballesteros-Tato, André; Browning, Jeffrey L; Dunn, Robert; Randall, Troy D; Lund, Frances E // Nature Immunology;Jul2012, Vol. 13 Issue 7, p681 

    Although cognate encounters between antigen-bearing dendritic cells (DCs) that express the chemokine receptor CCR7 and CCR7+ naive T cells take place in the T cell zone of lymph nodes, it is unknown whether the colocalization of DCs and T cells in the T cell area is required for the generation...

  • Effect of human double-stranded DNA preparation on the production of cytokines by dendritic cells and peripheral blood cells from relatively healthy donors. Orishchenko, Konstantin E.; Ryzhikova, Svetlana L.; Druzhinina, Yulia G.; Ryabicheva, Tatiana G.; Varaksin, Nikolay A.; Alyamkina, Ekaterina A.; Dolgova, Evgenia V.; Rogachev, Vladimir A.; Proskurina, Anastasia S.; Nikolin, Valeriy P.; Popova, Nelly A.; Strunov, Anton A.; Kiseleva, Elena V.; Leplina, Olga Y.; Ostanin, Alexandr A.; Chernykh, Elena R.; Sidorov, Sergey V.; Mayorov, Vladimir I.; Bogachev, Sergey S.; Shurdov, Mikhail A. // Cancer Therapy;2011, Vol. 8, p191 

    Nucleic acids of various origin and form are capable of activating the innate immune cells and inducing the development of the adaptive immune response. Here we demonstrated that fragments of double-stranded DNA (dsDNA) preparation reached the nuclear space of ex vivo generated human dendritic...

  • Dendritic Cell-Mediated Vaccination Relies on Interleukin-4 Receptor Signaling to Avoid Tissue Damage after Leishmania major Infection of BALB/c Mice. Masic, Anita; Hurdayal, Ramona; Nieuwenhuizen, Natalie E.; Brombacher, Frank; Moll, Heidrun // PLoS Neglected Tropical Diseases;Jul2012, Vol. 6 Issue 7, p1 

    Prevention of tissue damages at the site of Leishmania major inoculation can be achieved if the BALB/c mice are systemically given L. major antigen (LmAg)-loaded bone marrow-derived dendritic cells (DC) that had been exposed to CpG-containing oligodeoxynucleotides (CpG ODN). As previous studies...

  • Innate lymphoid cells wield a double-edged sword. Wills-Karp, Marsha; Finkelman, Fred D // Nature Immunology;Nov2011, Vol. 12 Issue 11, p1025 

    No abstract available.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics