TITLE

Validation of putative reference genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR

PUB. DATE
January 2008
SOURCE
BMC Genetics;2008, Vol. 9, p350
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
No abstract available.
ACCESSION #
43750458

 

Related Articles

  • A Panel of Stably Expressed Reference Genes for Real-Time qPCR Gene Expression Studies of Mallards (Anas platyrhynchos). Chapman, Joanne R.; Helin, Anu S.; Wille, Michelle; Atterby, Clara; Järhult, Josef D.; Fridlund, Jimmy S.; Waldenström, Jonas // PLoS ONE;2/17/2016, Vol. 11 Issue 2, p1 

    Determining which reference genes have the highest stability, and are therefore appropriate for normalising data, is a crucial step in the design of real-time quantitative PCR (qPCR) gene expression studies. This is particularly warranted in non-model and ecologically important species for which...

  • Functional and Topological Properties in Hepatocellular Carcinoma Transcriptome. Drozdov, Ignat; Bornschein, Jan; Wex, Thomas; Valeyev, Najl V.; Tsoka, Sophia; Malfertheiner, Peter // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    Hepatocellular carcinoma (HCC) is a leading cause of global cancer mortality. However, little is known about the precise molecular mechanisms involved in tumor formation and pathogenesis. The primary goal of this study was to elucidate genome-wide molecular networks involved in development of...

  • Functional and Topological Properties in Hepatocellular Carcinoma Transcriptome. Drozdov, Ignat; Bornschein, Jan; Wex, Thomas; Valeyev, Najl V.; Tsoka, Sophia; Malfertheiner, Peter // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    Hepatocellular carcinoma (HCC) is a leading cause of global cancer mortality. However, little is known about the precise molecular mechanisms involved in tumor formation and pathogenesis. The primary goal of this study was to elucidate genome-wide molecular networks involved in development of...

  • Analysis of gene expression patterns in hepatocellular carcinoma. Hui, Lijian; Wu, Tangmin; Xu, Liang; Hu, Gengxi // Nature Genetics;Nov99 Supplement, Vol. 23, p52 

    Presents an abstract for the article on the analysis of gene expression patterns in hepatocellular carcinoma.

  • Overexpression of NDRG1 is an indicator of poor prognosis in hepatocellular carcinoma. Chua, Mei-Sze; Sun, Hongbo; Cheung, Siu T; Mason, Veronica; Higgins, John; Ross, Douglas T; Fan, Sheung T; So, Samuel // Modern Pathology;Jan2007, Vol. 20 Issue 1, p76 

    No abstract available.

  • Expression and Purification of a Putative Tumor Suppressor Gene PP5715 in E. coli with Growth Inhibition of Hepatocellular Carcinoma Cells. Ma Wen-Zhe; Han Wei; Qin Wen-Xin; Wan Da-Fang; Gu Jian-Ren; Yang Sheng-Li; Gong Yi // Protein & Peptide Letters;Jan2006, Vol. 13 Issue 1, p41 

    PP5715 is a putative tumor suppressor gene that encodes a protein of 199 amino acids. Expression of PP5715 in E. coli was mainly as inclusion bodies. The recombinant protein was purified by a NTA-Ni2+ affinity column, refolded by dialysis, and shown to suppress growth of two human hepatocellular...

  • Gene Expression Profiling of Liver Cancer Stem Cells by RNA-Sequencing. Ho, David W. Y.; Zhen Fan Yang; Kang Yi; Chi Tat Lam; Ng, Michael N. P.; Wan Ching Yu; Joyce Lau; Timothy Wan; Xiaoqi Wang; Zhixiang Yan; Hang Liu; Yong Zhang; Sheung Tat Fan // PLoS ONE;May2012, Vol. 7 Issue 5, p1 

    Background: Accumulating evidence supports that tumor growth and cancer relapse are driven by cancer stem cells. Our previous work has demonstrated the existence of CD90+ liver cancer stem cells (CSCs) in hepatocellular carcinoma (HCC). Nevertheless, the characteristics of these cells are still...

  • Defining reference genes in Oryza sativa using organ, development, biotic and abiotic transcriptome datasets. Narsai, Reena; Ivanova, Aneta; Ng, Sophia; Whelan, James // BMC Plant Biology;2010, Vol. 10, p56 

    Background: Reference genes are widely used to normalise transcript abundance data determined by quantitative RTPCR and microarrays. However, the approaches taken to define reference genes can be variable. Although Oryza sativa (rice) is a widely used model plant and important crop specie, there...

  • Identification of Suitable Reference Genes for Real-Time PCR Analysis of Statin-Treated Human Umbilical Vein Endothelial Cells. ska-Granica, Barbara Żyżyń; Koziak, Katarzyna // PLoS ONE;Dec2012, Vol. 7 Issue 12, p1 

    Proper data normalization in quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) is of critical importance for reliable mRNA expression analysis. Due to a diversity in putative reference genes expression stability in different in vitro models, a validation of an...

  • De-regulation of common housekeeping genes in hepatocellular carcinoma. Waxman, Samuel; Wurmbach, Elisa // BMC Genomics;2007, Vol. 8, p243 

    Background: Tumorigenesis is associated with changes in gene expression and involves many pathways. Dysregulated genes include "housekeeping" genes that are often used for normalization for quantitative real-time RT-PCR (qPCR), which may lead to unreliable results. This study assessed eight...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics