High-throughput indentational elasticity measurements of hydrogel extracellular matrix substrates

D'Sa, Dexter J.; de Juan Pardo, Elena M.; de las Rivas Astiz, Rosalia; Sen, Shamik; Kumar, Sanjay
August 2009
Applied Physics Letters;8/10/2009, Vol. 95 Issue 6, p063701
Academic Journal
Much interest surrounds the effect of extracellular matrix (ECM) elasticity on cell behavior. Here we present a rapid method for measuring the elasticity of synthetic ECM substrates based on indentation of the substrate with a ferromagnetic sphere and optical tracking of the resulting deformation. We find that this method yields order-of-magnitude agreement with atomic force microscopy elasticity measurements, but that the degree of this agreement depends strongly on sphere density and gel elasticity. In its regime of greatest accuracy, we envision that this method may be used for high-throughput characterization of ECM substrates in cell biological studies.


Related Articles

  • Magnetization reversal processes in epitaxial Co/Fe bi-layers grown on MgO(001). Kohn, A.; Wang, C.; Petford-Long, A. K.; Wang, S. G.; Ward, R. C. C. // Journal of Applied Physics;Mar2008, Vol. 103 Issue 6, p063918 

    We report on the mechanism of magnetization reversal in epitaxial Co/Fe bi-layers grown by molecular beam epitaxy on MgO(001) substrates. For Co films thicker than 5 nm, the crystal structure is hexagonal. The Fe layer follows an epitaxial relation relative to the MgO substrate of...

  • Static magnetic field exposure promotes differentiation of osteoblastic cells grown on the surface of a poly- l-lactide substrate. Sheng-Wei Feng; Yi-June Lo; Wei-Jen Chang; Che-Tong Lin; Sheng-Yang Lee; Yoshimitsu Abiko; Haw-M ing Huang // Medical & Biological Engineering & Computing;Aug2010, Vol. 48 Issue 8, p793 

    This study investigated the effects of static magnetic fields on the differentiation of MG63 cells cultured on the surface of poly- l-lactide (PLLA) substrates. The cells were continuously exposed to a 4,000 Gauss-static magnetic field (SMF) for 5 days. The proliferation effects of the SMF were...

  • Surface Energy Matchmaking. Miller, Mark // Paper, Film & Foil Converter;Aug2011, Vol. 85 Issue 8, p12 

    The article discusses ways on how to harmoniously combine fluid and substrate to properly matched surface energy. It mentions that it is beneficial to increase the surface energy of the substrate and decrease the surface energy of the fluid as well as promote adhesion at the coating interface....

  • Elasticity moduli of CdHgTe solid solutions. Nikiforov, V. N.; Sredin, V. G.; Kurbanov, K. R. // Russian Physics Journal;Aug2011, Vol. 54 Issue 3, p271 

    Results of investigations into the temperature dependences (4.2-300 K) of the low-frequency ultrasound velocity in CdHgTe single crystals are presented. The elastic moduli and the Debye temperatures are calculated for different solid solution compositions. Based on the data obtained, possible...

  • The competition between the liquid-liquid dewetting and the liquid-solid dewetting. Lin Xu; Tongfei Shi; Lijia An // Journal of Chemical Physics;5/14/2009, Vol. 130 Issue 18, p184903 

    We investigate the dewetting behavior of the bilayer of air/PS/PMMA/silanized Si wafer and find the two competing dewetting pathways in the dewetting process. The upper layer dewets on the lower layer (dewetting pathway 1, the liquid-liquid dewetting) and the two layers rupture on the solid...

  • Investigations on interfacial reactions at reentrant corners. Chao-Hong Wang; Chen-Nan Chiu; Sinn-Wen Chen // Journal of Materials Research;May2010, Vol. 25 Issue 5, p26 

    Interfacial reactions in Bi/Ni, Sn/Co, and Sn/Te systems that exhibit unique cruciform pattern formation are investigated. Different from the couples examined in the past, the solid substrates, Ni, Co, and Te, are placed outside the couples while constituents of low melting temperature, Bi and...

  • The Origin of Local Strain in Highly Epitaxial Oxide Thin Films. Ma, Chunrui; Ming Liu; Chonglin Chen; Yuan Lin; Yanrong Li; Horwitz, J. S.; Jiang, Jiechao; Meletis, E. I.; Qingyu Zhang // Scientific Reports;11/1/2013, p1 

    The ability to control the microstructures and physical properties of hetero-epitaxial functional oxide thin films and artificial structures is a long-sought goal in functional materials research. Normally, only the lattice misfit between the film and the substrate is considered to govern the...

  • Bandgap bowing in BGaN thin films. Ougazzaden, A.; Gautier, S.; Moudakir, T.; Djebbour, Z.; Lochner, Z.; Choi, S.; Kim, H. J.; Ryou, J.-H.; Dupuis, R. D.; Sirenko, A. A. // Applied Physics Letters;8/25/2008, Vol. 93 Issue 8, p083118 

    We report on the bandgap variation in thin films of BxGa1-xN grown on AlN/sapphire substrates using metal-organic vapor phase epitaxy. Optical transmission, photoluminescence, and x-ray diffraction were utilized to characterize the materials’ properties of the BxGa1-xN films. In contrast...

  • Modeling universal dynamics of cell spreading on elastic substrates. Fan, Houfu; Li, Shaofan // Biomechanics & Modeling in Mechanobiology;Nov2015, Vol. 14 Issue 6, p1265 

    A three-dimensional (3D) multiscale moving contact line model is combined with a soft matter cell model to study the universal dynamics of cell spreading over elastic substrates. We have studied both the early stage and the late stage cell spreading by taking into account the actin tension...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics