TITLE

High-throughput indentational elasticity measurements of hydrogel extracellular matrix substrates

AUTHOR(S)
D'Sa, Dexter J.; de Juan Pardo, Elena M.; de las Rivas Astiz, Rosalia; Sen, Shamik; Kumar, Sanjay
PUB. DATE
August 2009
SOURCE
Applied Physics Letters;8/10/2009, Vol. 95 Issue 6, p063701
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Much interest surrounds the effect of extracellular matrix (ECM) elasticity on cell behavior. Here we present a rapid method for measuring the elasticity of synthetic ECM substrates based on indentation of the substrate with a ferromagnetic sphere and optical tracking of the resulting deformation. We find that this method yields order-of-magnitude agreement with atomic force microscopy elasticity measurements, but that the degree of this agreement depends strongly on sphere density and gel elasticity. In its regime of greatest accuracy, we envision that this method may be used for high-throughput characterization of ECM substrates in cell biological studies.
ACCESSION #
43720441

 

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