TITLE

Ion-pairing high-performance liquid chromatography determination of amitrole in apple after solid-phase extraction and precolumn derivatization

AUTHOR(S)
Yan Sun; Laixin Luo; Fang Wang; Jianqiang Li; Yongsong Cao
PUB. DATE
September 2009
SOURCE
Analytical & Bioanalytical Chemistry;Sep2009, Vol. 395 Issue 2, p465
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A novel method based on solid-phase extraction was studied for the extraction of amitrole (3-amino-1,2,4-triazole), and its residue determination in apples has been developed. The samples were derivatized with 4-chloro-3,5-dinitrobenzotrifluoride (CNBF). The derivatization conditions and the influence of elution composition on the separation were investigated. In pH 9.5 H3BO3–Na2B4O7 media, the reaction of amitrole with CNBF was complete at 60°C after 30 min. The separation of derivatized amitrole was achieved at room temperature within 13 min by gradient elution mode with cetyltrimethylammonium bromide in mobile phase as ion-pair reagent. The method correlation coefficient was 0.9996, in concentrations ranging from 1.66 to 415 mg L−1. The calculated recoveries of the proposed method were from 94.17% to 105.67%, and relative standard deviations were 1.57% to 6.44% in the application to the quantitative determination of amitrole in apples. The detection limit of amitrole was 0.10 mg L−1 with a signal-to-noise ratio of 3. [Figure not available: see fulltext.]
ACCESSION #
43707220

 

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