Molecularly imprinted monolith in-tube solid-phase microextraction coupled with HPLC/UV detection for determination of 8-hydroxy-2′-deoxyguanosine in urine

Shao-Wen Zhang; Jun Xing; Ling-Shuang Cai; Cai-Ying Wu
September 2009
Analytical & Bioanalytical Chemistry;Sep2009, Vol. 395 Issue 2, p479
Academic Journal
Urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) has been widely used as a biomarker of oxidative DNA damage. Measurements of 8-OHdG in urinary samples are challenging owing to the low level of 8-OHdG and the complex matrix. In this study, a novel molecularly imprinted polymer (MIP) monolithic column was synthesized with guanosine as a dummy template which was used as the medium for in-tube solid-phase microextraction (SPME). In-tube SPME coupled with HPLC/UV detection for extraction and determination of urinary 8-OHdG was developed. The synthesized MIP monolithic column exhibited high extraction efficiency owing to its greater phase ratio with convective mass transfer and inherent selectivity. The enrichment factor for 8-OHdG was found to be 76 and the limits of detection and quantification of the method for urinary samples were 3.2 nmol/L (signal-to-noise ratio 3) and 11 nmol/L (signal-to-noise ratio 10), respectively. The MIP’s selectivity also made the sample preparation procedure and chromatographic separation much easier. The linear range of the proposed method was from 0.010 to 5.30 μmol/L ( r = 0.9997), with a relative standard deviation of 1.1–6.8%, and the recovery for spiked urine samples was 84 ± 3%. The newly developed method was successfully applied to determine urinary samples of healthy volunteers, coking plant workers, and cancer patients. The 8-OHdG level in cancer patients was significantly higher than that in healthy people.


Related Articles

  • Automated Sample Preparation Platform for Mass Spectrometry-Based Plasma Proteomics and Biomarker Discovery. Guryča, Vilém; Roeder, Daniel; Piraino, Paolo; Lamerz, Jens; Ducret, Axel; Langen, Hanno; Cutler, Paul // Biology (2079-7737);Mar2014, Vol. 3 Issue 1, p205 

    The identification of novel biomarkers from human plasma remains a critical need in order to develop and monitor drug therapies for nearly all disease areas. The discovery of novel plasma biomarkers is, however, significantly hampered by the complexity and dynamic range of proteins within...

  • Patients with ovarian carcinoma excrete different altered levels of urine CD59, kininogen-1 and fragments of inter-alpha-trypsin inhibitor heavy chain H4 and albumin. Abdullah-Soheimi, Siti S.; Boon-Kiong Lim; Hashim, Onn H.; Shuib, Adawiyah S. // Proteome Science;2010, Vol. 8, p58 

    Background: Diagnosis of ovarian carcinoma is in urgent need for new complementary biomarkers for early stage detection. Proteins that are aberrantly excreted in the urine of cancer patients are excellent biomarker candidates for development of new noninvasive protocol for early diagnosis and...

  • Vinyl sulfone silica: application of an open preactivated support to the study of transnitrosylation of plant proteins by S-nitrosoglutathione. Begara-Morales, Juan C.; López-Jaramillo, F Javier; Sánchez-Calvo, Beatriz; Carreras, Alfonso; Ortega-Muñoz, Mariano; Santoyo-González, Francisco; Corpas, Francisco J.; Barroso, Juan B. // BMC Plant Biology;2013, Vol. 13 Issue 1, p1 

    Background: S-nitrosylaton is implicated in the regulation of numerous signaling pathways with a diversity of regulatory roles. The high lability of the S-NO bond makes the study of proteins regulated by S-nitrosylation/ denitrosylation a challenging task and most studies have focused on already...

  • Diagnostic and prognostic value of bladder cancer-related transcript markers in urine. Schmidt, Juliane; Propping, Catharina; Siow, Woei-Yun; Lohse-Fischer, Andrea; Toma, Marieta; Baldauf-Twelker, Anka; Hakenberg, Oliver; Wirth, Manfred; Fuessel, Susanne // Journal of Cancer Research & Clinical Oncology;Feb2016, Vol. 142 Issue 2, p401 

    Purpose: Since cytology as the current 'gold standard' for noninvasive detection of bladder cancer (BCa) is characterized by a relatively low sensitivity, urinary transcript levels of survivin (SVV), Ki-67 and cytokeratin 20 (CK20) were evaluated as alternative or complementary biomarkers....

  • Curated MicroRNAs in Urine and Blood Fail to Validate as Predictive Biomarkers for High-Risk Prostate Cancer. Sapre, Nikhil; Hong, Matthew K. H.; Macintyre, Geoff; Lewis, Heather; Kowalczyk, Adam; Costello, Anthony J.; Corcoran, Niall M.; Hovens, Christopher M. // PLoS ONE;Apr2014, Vol. 9 Issue 4, p1 

    Purpose: The purpose of this study was to determine if microRNA profiling of urine and plasma at radical prostatectomy can distinguish potentially lethal from indolent prostate cancer. Materials and Methods: A panel of microRNAs was profiled in the plasma of 70 patients and the urine of 33...

  • Drug Target Exploitable Structural Features of Adenylyl Cyclase Activity in Schistosoma mansoni. Mbah, Andreas N.; Kamga, Henri L.; Awofolu, Omotayo R.; Isokpehi, Raphael D. // Drug Target Insights;2012, Issue 6, p41 

    The draft genome sequence of the parasitic flatworm Schistosoma mansoni (S. mansoni), a cause of schistosomiasis, encodes a predicted guanosine triphosphate (GTP) binding protein tagged Smp_059340.1. Smp_059340.1 is predicted to be a member of the G protein alpha-s subunit responsible for...

  • Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis. Yuanbin Zhang; Jing Zhao; Xinye Wang; Xindong Xu; Weiqing Pan // Parasites & Vectors;9/4/2015, Vol. 8 Issue 1, p1 

    Background: Early diagnosis of schistosomiasis, prior to egg laying, would enable earlier treatment and help interrupt the transmission cycle of the parasite and the progress of the disease. Previously we identified six novel antigens with potential as diagnostic markers for human Schistosoma...

  • Metronomics in low and middle income countries: India showing the way! André, N.; Pasquier, E. // Indian Journal of Cancer;Apr-Jun2013, Vol. 50 Issue 2, p112 

    An introduction is presented in which the editor discusses various reports within the issue on topics including the need for biomarkers to patients monitoring, the esophageal cancer treatments and the use of metronomic weekly paclitaxel on non-small-cell lung cancer.

  • Serum metabolomics as a novel diagnostic approach for pancreatic cancer. Nishiumi, Shin; Shinohara, Masakazu; Ikeda, Atsuki; Yoshie, Tomoo; Hatano, Naoya; Kakuyama, Saori; Mizuno, Shigeto; Sanuki, Tsuyoshi; Kutsumi, Hiromu; Fukusaki, Eiichiro; Azuma, Takeshi; Takenawa, Tadaomi; Yoshida, Masaru // Metabolomics;Dec2010, Vol. 6 Issue 4, p518 

    Pancreatic cancer is one of the leading causes of cancer-related death, and there is currently little hope of a cure because there are no effective biomarkers for its early detection. Therefore, the search for novel biomarkers that would allow the early detection of pancreatic cancer is ongoing....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics