TITLE

Transformation of Brazilian Elite Indica-Type Rice (Oryza Sativa L.) by Electroporation of Shoot Apex Explants

AUTHOR(S)
Muniz de Padua, Vania L.; Ferreira, Rodrigo P.; Meneses, Larissa; Uchoa, Nathaly; Margis-Pinheiro, Marcia; Mansur, Elisabeth
PUB. DATE
March 2001
SOURCE
Plant Molecular Biology Reporter;Mar2001, Vol. 19 Issue 1, p55
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract. We have generated transgenic plants of a Brazilian elite Indica-type rice by electroporation of shoot apices. This approach avoids a callus phase and produces 0.4-13.8% resistant plants. Transgenic plantlets were transferred to soil a few weeks after explant electroporation. Root segments from plantlets obtained from transformation experiments with pAHC25 plasmid were GUS positive. Integration of the introduced gene into the genome was demonstrated by PPT and antibiotic screening as well as by PCR and Southern blot hybridization of genomic DNA isolated from R[sub 2] plantlets.
ACCESSION #
4362334

 

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