The Effects of Fibroblast Growth Factors 1 and 2 on Fibre Growth of Wool Follicles in Culture

Bond, J. J.; Wynn, P. C.; Moore, G. P. M.
September 1998
Acta Dermato-Venereologica;09/01/98, Vol. 78 Issue 5, p337
Academic Journal
The effects of fibroblast growth factor-1 (FGF-1) and FGF-2 on fibre growth and follicle function were examined using a previously described procedure to culture wool follicles. Because the FGFs bind to glycosaminoglycan components of the extra-cellular matrix, we also investigated interactions between FGF-1 and FGF-2 with heparin on wool fibre growth. Individual follicles were microdissected from Merino sheepskin and transferred to culture. Follicles increased in length for 6 days, and in all groups, no significant differences in follicle length were observed. Increase in follicle length was associated with fibre growth, follicles maintained normal anagen morphology and incorporated [[sup 3]H]thymidine into the bulb and outer root sheath cells. Follicles in all treatments continued to produce fibre keratins, as detected by immunohistochemistry. However, the patterns of fibre and cytoskeletal proteins incorporating [[sup 35]S]methionine in control and treated follicles were significantly different. We found a considerable decrease in the intermediate filament keratins or low sulphur proteins in follicles cultured in the presence of FGF-1 and FGF-2 compared to controls. The majority of proteins detected in these samples were acidic high sulphur proteins. These studies provide evidence for a specific role for the fibroblast growth factors in the regulation of fibre differentiation.


Related Articles

  • Dermal disorders: Boosting hair follicle regeneration. Charlotte, Harrison // Nature Reviews Drug Discovery;Aug2013, Vol. 12 Issue 8, p580 

    The article presents a research that investigates the vital role of fibroblast growth factor 9 (FGF9) in the regeneration of hair follicles after the healing of skin wounds in mice.

  • Bulge- and Basal Layer-Specific Expression of Fibroblast Growth Factor-13 (FHF-2) in Mouse Skin. Kawano, Mitsuko; Suzuki, Satoshi; Suzuki, Masashi; Oki, Junko; Imamura, Toru // Journal of Investigative Dermatology;May2004, Vol. 122 Issue 5, p1084 

    A variety of polypeptide growth factors are involved in the dynamic maintenance of the skin and hair. Here, we demonstrate the presence of high levels of fibroblast growth factor (FGF)-13 in the bulge region of hair follicles. Using real-time PCR, we found that expression of FGF-13 mRNA is...

  • New Activators and Inhibitors in the Hair Cycle Clock: Targeting Stem Cells' State of Competence. Plikus, Maksim V // Journal of Investigative Dermatology;May2012, Vol. 132 Issue 5, p1321 

    The timing mechanism of the hair cycle remains poorly understood. However, it has become increasingly clear that the telogen-to-anagen transition is controlled jointly by at least the bone morphogenic protein (BMP), WNT, fibroblast growth factor (FGF), and transforming growth factor (TGF)-β...

  • High-efficiency production of bioactive recombinant human fibroblast growth factor 18 in Escherichia coli and its effects on hair follicle growth. Song, Lintao; Huang, Zhifeng; Chen, Yu; Li, Haiyan; Jiang, Chao; Li, Xiaokun // Applied Microbiology & Biotechnology;Jan2014, Vol. 98 Issue 2, p695 

    Using fusion tags, expression of recombinant human fibroblast growth factor 18 (rhFGF18) in mammalian cells and Escherichia coli has been extensively used for fundamental research and clinical applications, including chondrogenesis and osteogenesis, hair growth, and neuroprotection. However,...

  • Fibroblast Growth Factors Stimulate Hair Growth through β-Catenin and Shh Expression in C57BL/6 Mice. Lin, Wei-hong; Xiang, Li-Jun; Shi, Hong-Xue; Zhang, Jian; Jiang, Li-ping; Cai, Ping-tao; Lin, Zhen-Lang; Lin, Bei-Bei; Huang, Yan; Zhang, Hai-Lin; Fu, Xiao-Bing; Guo, Ding-Jiong; Li, Xiao-Kun; Wang, Xiao-Jie; Xiao, Jian // BioMed Research International;1/1/2015, Vol. 2015, p1 

    Growth factors are involved in the regulation of hair morphogenesis and cycle hair growth. The present study sought to investigate the hair growth promoting activities of three approved growth factor drugs, fibroblast growth factor 10 (FGF-10), acidic fibroblast growth factor (FGF-1), and basic...

  • Characterization of a Hair (Wool) Keratin Intermediate Filament Gene Domain. Powell, Barry C.; Beltrame, Juliana S. // Journal of Investigative Dermatology;Feb94, Vol. 102 Issue 2, p171 

    In epithelial differentiation keratin intermediate filament genes are expressed in multifarious tissue-specific and stage-specific patterns. Pairs of type I and type II intermediate filament genes, belonging to multigene families, are coordinately regulated, and 4 - 5 genes of each type are...

  • Hair Cycle Resting Phase Is Regulated by Cyclic Epithelial FGF18 Signaling. Kimura-Ueki, Miho; Oda, Yuko; Oki, Junko; Komi-Kuramochi, Akiko; Honda, Emi; Asada, Masahiro; Suzuki, Masashi; Imamura, Toru // Journal of Investigative Dermatology;May2012, Vol. 132 Issue 5, p1338 

    Hair follicles repeatedly cycle through growth (anagen), regression (catagen), and resting (telogen) phases. Although the signaling molecules involved in the anagen and anagen-catagen transition have been studied extensively, the signaling that controls telogen is only partly understood. Here we...

  • Functional Characterization of Melanocyte Stem Cells in Hair Follicles. Nishikawa-Torikai, Satomi; Osawa, Masatake; Nishikawa, Shin-ichi // Journal of Investigative Dermatology;Dec2011, Vol. 131 Issue 12, p2358 

    In mice, coat pigmentation requires a stem cell (SC) system in which the survival, proliferation, and differentiation of melanocytes (MCs) are regulated by microenvironments in hair follicles (HFs). In vitro systems are required to analyze the behavior of single melanocyte stem cells (MCSCs) and...

  • Fibroblast Growth Factor and Epidermal Growth Factor in Hair Development. du Cros, Diana Lee // Journal of Investigative Dermatology;Jul93 Supplement, Vol. 101, p106S 

    Hair follicles arise in developing skin as a result of a complex of interactions that are likely to be mediated by diffusible, cell- and matrix-bound factors. Growth factors such as fibroblast growth factor (FGF) and epidermal growth factor (EGF) have been implicated in the control of epidermal...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics