TITLE

Gradient HPLC separation of dehydroepiandrosterone (DHEA) from its metabolites and biological congeners: role of tetrahydrofuran in the chromatographic mechanism

AUTHOR(S)
Gergely, András; Horváth, Péter; Szász, György; Veress, Gábor
PUB. DATE
August 2009
SOURCE
Analytical & Bioanalytical Chemistry;Aug2009, Vol. 394 Issue 8, p2105
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A three-step gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for the separation of dehydroepiandrosterone (DHEA), its sulfate ester (DHEA-S), its three C7-oxidized metabolites (7αOH-DHEA, 7βOH-DHEA, 7-keto-DHEA), and its biosynthetic congeners (androstenedione, testosterone, estradiol, pregnenolone). This new method allows the quantitative characterization of DHEA metabolism and biosynthetic transformation under given physiological, pathological, or therapeutically influenced circumstances. Tetrahydrofuran probably acts as a proton acceptor coadsorbent, while isopropanol behaves as a proton donor during the separation of testosterone, estradiol, and the stereoisomers of 7-OH-DHEA. [Figure not available: see fulltext.]
ACCESSION #
43467488

 

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