TITLE

Interleukin-15 is not a Constitutive Cytokine in the Epidermis, but is Inducible in Culture or Inflammatory Conditions

AUTHOR(S)
Han, Gang-Wen; Iwatsuki, Keiji; Inoue, Masayuki; Matsui, Takashi; Nishibu, Akiko; Akiba, Hitoshi; Kaneko, Fumio
PUB. DATE
March 1999
SOURCE
Acta Dermato-Venereologica;03/18/99, Vol. 79 Issue 1, p37
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The regulation in the skin of interleukin-15 (IL-15), a potent modulator of T-cell-mediated immune responses, is not fully understood. We investigated the levels of IL-15 and its mRNA produced by epidermal and cultured keratinocytes and found that normal keratinocytes did not constitutively express IL-15 in the epidermis, but in culture began to produce the cytokine. Some epidermal keratinocytes expressed IL-15 in inflammatory conditions associated with infiltration of neutrophils and eosinophils. IL-15 was detected only in the cell lysates, not in the supernatants of cultured keratinocytes. Dexamethasone (10-[sup 5]-10-[sup 6] M) markedly inhibited IL-15 mRNA expression by normal and transformed keratinocytes in a range of pharmacological concentrations. IFN-γ (200 and 400 U/ml) slightly increased the IL-15 message level in a squamous cell carcinoma cell line, HSC-5, in a dose-dependent fashion, whereas no significant change was observed in cultured normal human keratinocytes. Our data indicate that IL-15 is not a constitutive cytokine in epidermal keratinocytes but is inducible.
ACCESSION #
4342553

 

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