TITLE

A Rat Excised Larynx Model of Vocal Fold Scar

AUTHOR(S)
Welham, Nathan V.; Montequin, Douglas W.; Tateya, Ichiro; Tateya, Tomoko; Seong Hee Choi; Bless, Diane M.
PUB. DATE
August 2009
SOURCE
Journal of Speech, Language & Hearing Research;Aug2009, Vol. 52 Issue 4, p1008
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: To develop and evaluate a rat excised larynx model for the measurement of acoustic, aerodynamic, and vocal fold vibratory changes resulting from vocal fold scar. Method: Twenty-four 4-month-old male Sprague--Dawley rats were assigned to 1 of 4 experimental groups: chronic vocal fold scar, chronic vocal fold scar treated with 100-ng basic fibroblast growth factor (bFGF), chronic vocal fold scar treated with saline (sham treatment), and unscarred untreated control. Following tissue harvest, histological and immunohistochemical data were collected to confirm extracellular matrix alteration in the chronic scar group; acoustic, aerodynamic, and high-speed digital imaging data were collected using an excised larynx setup in all groups. Phonation threshold pressure (Pth), glottal resistance (Rg), glottal efficiency (Eg), vibratory amplitude, and vibratory area were used as dependent variables. Results: Chronically scarred vocal folds were characterized by elevated collagen Types I and III and reduced hyaluronic acid abundance. Phonation was achieved, and data were collected from all control and bFGF-treated larynges; however, phonation was not achieved with 3 of 6 chronically scarred and 1 of 6 saline-treated larynges. Compared with control, the chronic scar group was characterized by elevated Pth, reduced Eg, and intralarynx vibratory amplitude and area asymmetry. The bFGF group was characterized by Pth below control-group levels, Eg comparable with control, and vocal fold vibratory amplitude and area symmetry comparable with control. The sham group was characterized by Pth comparable with control, Eg superior to control, and vocal fold vibratory amplitude and area symmetry comparable with control. Conclusions: The excised larynxmodel reported here demonstrated robust deterioration across phonatory indices under the scar condition and sensitivity to treatment-induced change under the bFGF condition. The improvement observed under the sham condition may reflect unanticipated therapeutic benefit or artifact. This model holds promise as a tool for the functional characterization of biomechanical tissue changes resulting from vocal fold scar and the evaluation of experimental therapies.
ACCESSION #
43421296

 

Related Articles

  • Nimodipine Improves Reinnervation and Neuromuscular Function After Injury to the Recurrent Laryngeal Nerve in the Rat. Hydman, Jonas; Remahl, Sten; Björck, Gunnar; Svensson, Mikael; Mattsson, Per // Annals of Otology, Rhinology & Laryngology;Aug2007, Vol. 116 Issue 8, p623 

    Objectives: Injury of the recurrent laryngeal nerve (RLN) is associated with a high degree of neuronal survival, but leads to various levels of vocal fold motion impairment or laryngeal synkinesis, which has been attributed to misdirected reinnervation of the target muscles in the larynx or...

  • Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats. Gugatschka, Markus; Ainödhofer, Herwig; Gruber, Hans-Jürgen; Graupp, Matthias; Kieslinger, Petra; Kiesler, Karl; Saxena, Amulya; Hirano, Shigeru; Friedrich, Gerhard // European Archives of Oto-Rhino-Laryngology;May2014, Vol. 271 Issue 5, p1107 

    Vocal fold (VF) fibroblasts are the central subject of interest in fibrogenesis and wound healing after VF injury. Scar fibroblasts (SF) exhibit an aberrant production of several extracellular matrix (ECM) components which lead either to VF fibrosis or scarless wound healing. This study aimed to...

  • Age-related changes in rat intrinsic laryngeal muscles: analysis of muscle fibers, muscle fiber proteins, and subneural apparatuses. Nishida, Naoya; Taguchi, Aki; Motoyoshi, Kazumi; Hyodo, Masamitsu; Gyo, Kiyofumi; Desaki, Junzo // European Archives of Oto-Rhino-Laryngology;Mar2013, Vol. 270 Issue 3, p975 

    We compared age-related changes in the intrinsic laryngeal muscles of aged and young adult rats by determining the number and diameter of muscle fibers, contractile muscle protein (myosin heavy chain isoforms, MHC) composition, and the morphology of the subneural apparatuses. In aged rats, both...

  • Expression of transcripts for fibroblast growth factor 18 and its possible receptors during postnatal dentin formation in rat molars. Baba, Otto; Ota, Masato; Terashima, Tatsuo; Tabata, Makoto; Takano, Yoshiro // Odontology;May2015, Vol. 103 Issue 2, p136 

    Fibroblast growth factors (FGFs) regulate the proliferation and differentiation of various cells via their respective receptors (FGFRs). During the early stages of tooth development in fetal mice, FGFs and FGFRs have been shown to be expressed in dental epithelia and mesenchymal cells at the...

  • REGENERATION OF AGED VOCAL FOLDS WITH BASIC FIBROBLAST GROWTH FACTOR IN A RAT MODEL: A PRELIMINARY REPORT. Shigeru Hirano; Hiromi Nagai; Ichiro Tateya; Tomoko Tatfya; Ford, Charles N.; Bless, Diane M. // Annals of Otology, Rhinology & Laryngology;Apr2005, Vol. 114 Issue 4, p304 

    Aged vucal folds have been reported to have dense collagen deposition and decreased hyaluronic acid (HA) in the lamina propria. These characteristics are thought to contribute to vocal problems that occur with age (presbyphonia). To restore better viscoelasticity to aged vocal folds, an...

  • Characterization of recurrent laryngeal nerve function in rats. Naggar, I.; Mor, N.; Nakase, K.; Zolkind, P.; Silverman, J. B.; Sundaram, K.; Stewart, M.; Kollmar, R. // Proceedings of the Physiological Society;2013, p850P 

    The recurrent laryngeal nerve (RLN) is a branch of the vagus nerve that controls the movement of the vocal folds and arytenoid cartilages. Since it is damaged in 4-10% of thyroid surgeries, a better characterization of its function may aid in finding ways to repair the consequent vocal fold...

  • SECTION 4: THE VOICE. Silverstein, Alvin; Silverstein, Virginia; Silverstein, Robert // Respiratory System (9780805028317);1994, p52 

    This article discusses how voices are made. Air flowing through the opening of the vocal cords that is stretched across the larynx makes the vocal cords vibrate. The tighter vocal cords are stretched, the higher the pitch of the sound. Irritation of the vocal cords can lower the pitch of the...

  • The vocal cords. Farndon, John // Human Body;2003, p59 

    Speaking and singing depend on the larynx in the neck. When a person speaks or sings, the vocal cords tighten across the airway and vibrate to make sounds. Women's vocal cords are about 0.8 inches long, while those of men are about 1.2 inches long. Because the vocal cords of men are longer...

  • The well-positioned endotracheal tube. Cullen, David J.; Newbower, Ronald S.; Gemer, Mordecai // RN;Apr76, Vol. 39 Issue 4, pOR8 

    Provides information on a method based on electromagnetic sensing for insertion of an endotracheal tube in patients requiring nasotracheal intubation. Procedure for the insertion of the tube; Distance from the marker band to the tip of the endotracheal tube; Commercial cost of adding the marker...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics