TITLE

High cell density fermentation of Gluconobacter oxydans DSM 2003 for glycolic acid production

AUTHOR(S)
Guodong Wei; Xuepeng Yang; Tula Gan; Wenyu Zhou; Jinping Lin; Dongzhi Wei
PUB. DATE
August 2009
SOURCE
Journal of Industrial Microbiology & Biotechnology;Aug2009, Vol. 36 Issue 8, p1029
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Gluconobacter oxydans has a lower biomass yield. Uniform design (UD) was applied to determine the optimum composition of the critical media and their mutual interactions for increased biomass yield of Gluconobacter oxydans DSM 2003 in shake flasks. Fed-batch fermentation process for biomass was optimized in a 3.7-l fermentor. By undertaking a preliminary and improved fed-batch fermentation-process strategy, a cell density of 6.0 g/l (DCW) was achieved in 22 h and 14.1 g/l (DCW) in 35 h, which is the highest cell density of G. oxydans produced thus far in a 3.7-l bioreactor. The biomass production was increased by 135% compared with that using the original cultivation strategy. Bioconversion of ethylene glycol to glycolic acid was catalyzed by the resting cells of G. oxydans DSM 2003, and conversion rate reached 86.7% in 48 h. In summary, the approach including high-density fermentation of G. oxydans DSM 2003 and bioconversion process was established and proved to be an effective method for glycolic acid production.
ACCESSION #
43320186

 

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