TITLE

Three-dimensional localization with nanometer accuracy using a detector-limited double-helix point spread function system

AUTHOR(S)
Pavani, Sri Rama Prasanna; Greengard, Adam; Piestun, Rafael
PUB. DATE
July 2009
SOURCE
Applied Physics Letters;7/13/2009, Vol. 95 Issue 2, p021103
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Accurate estimation of the three-dimensional (3D) position of particles is critical in applications like biological imaging, atom/particle-trapping, and nanomanufacturing. While it is well-known that localization accuracy better than the Rayleigh resolution limit is possible, it was recently shown that, for photon-limited cases, 3D point spread functions (PSFs) can be shaped to increase accuracies over a 3D volume [Pavani and Piestun, Opt. Express 16, 22048 (2008)]. Here, we show that in the detector-limited regime, the gain in accuracy occurs in all three dimensions throughout the axial range of interest. The PSF is shaped as a double helix, resulting in a system with fundamentally better 3D localization accuracies than standard PSF systems, capable of achieving single-image subnanometer accuracies.
ACCESSION #
43277425

 

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