Tear ascorbic acid levels and the total antioxidant status in contact lens wearers: A pilot study

Venkata, Sai Jyothi Aluru; Narayanasamy, Angayarkanni; Srinivasan, Vidhya; Iyer, Geetha Krishnan; Sivaramakrishnan, Ramakrishnan; Subramanian, Madhumathi; Mahadevan, Rajeshwari
September 2009
Indian Journal of Ophthalmology;Sep2009, Vol. 57 Issue 4, p289
Academic Journal
Aims: The tear ascorbate owing to its high concentration, functions as an effective antioxidant against the oxidative damage of cornea. Contact lens wearers (CLW) are prone to oxidative stress due to the lens-induced hypoxic conditions. A pilot study was done to compare the tear ascorbic acid level and the total antioxidant capacity give as in normal and CLW. Materials and Methods: In this study 21 CLW (Mean age 23 ± 3 years; M-2, F-19), who were daily wear users, with duration of wear not more than four years, along with age-matched 28 controls (Mean age 28 ± 3; M-15, F-13) were recruited in the study for collection of reflex tears using Schirmer's strip. Ascorbic acid in tears was determined using high-performance liquid chromatography (HPLC), total antioxidant capacity (TAC) and total protein assay by spectrophotometric analysis. Results: CLW showed no significant change in the tear ascorbic acid levels (0.4 ± 0.26 mM) compared to the control subjects (0.61 ± 0.59 mM). The amount of ascorbic acid in tears did not correlate with the TAC or the total protein of the tears. The mean TAC in CLW was 0.69 ± 0.16 mM, with a total protein of 1.35 ± 0.46 mg/ml while in controls it was 0.7 ± 0.18 mM and 1.21 ± 0.47 mg/ml respectively. Conclusions: Soft contact lens wear did not show any significant change in tear ascorbic acid, TAC and total protein levels compared to controls.


Related Articles

  • Guide to Fitting GP Multifocals and Bifocals. Od, Ed Bennett // Review of Optometry;5/15/2004 Supplement, p15 

    Part I. Provides a guide for gas permeable (GP) presbyopic contact lens correction. Taking a patient history; Measurement of palpebral fissure size, pupil size, lid-to-limbus relationship and lid tonicity to determine the appropriate design and lens diameter; Evaluation of prospective GP...

  • The Balancing Act. Rinehart, John M. // Review of Optometry;Oct2010 Review of Cornea & Contact, p7 

    The article discusses the factors that could induce peripheral corneal staining (PCS) and establish a balance tear and fitting of contact lenses for patients in a long-term safety. He emphasized that insufficient peripheral clearance, too little edge lift and lens-cornea fitting relationship are...

  • A Lens Fit for Dry Eye. Subbaraman, Lakshman N.; Srinivasan, Sruthi // Review of Optometry;Mar2013 Review of Cornea & Contact Lenses, p15 

    The article presents clinical methods for the treatment of contact lens patients having dry eye. It informs about Contact lens-related dry eye (CLDE) is a complex and multifactorial eye problem including increased tear evaporation, lens deposits and altered tear osmolarity which exacerbate dry...

  • Solution bio-inspired to meet needs of your contemporary practice better. Giedd, Kerry // Optometry Times;Nov/Dec2010, Vol. 2 Issue 10, p12 

    The article describes an inventive multi-purpose solution (MPS) that benefits soft contact-lens wearing patients. According to the author, the new MPS called Biotrue from Bausch and Lomb is formulated to copy the natural chemistry of the eye. She notes that Biotrue will match the pH of human...

  • Cold Eye Comfort. Parajon, Lauren // Prevention;Jan2009, Vol. 61 Issue 1, p28 

    The article reports that researchers at the University of Alabama at the Birmingham School of Optometry recommend wearing eyeglasses instead of contact lenses when suffering from a cold. The author explains that contact lens wearers are more prone to conjunctivitis when there is a decline in...

  • Good Tears and Bad Tears. Hom, Milton M.; Simmons, Peter // Review of Optometry;2/15/2004, Vol. 141 Issue 2, p84 

    Examines the role of tears in contact lens-related dryness in the United States. Composition and quality of the tear film; Inflammatory factors related to dryness; Redness as a hallmark sign of inflammation.

  • How Lens Care Products Stack Up.  // Review of Optometry;5/15/2002 Supplement, Vol. 139, p46 

    Explores different cleaning differences in lens care products. Effect of contact lens care regimen on lysozyme removal; Quantification of protein levels by high performance liquid chemotherapy; Efficiency of the no rub opti-free express.

  • Correction: Yan, R.Y., et al. HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli. Molecules 2014, 19, 4409-4417.  // Molecules;Jan2016, Vol. 21 Issue 1, p125 

    A correction to the article "HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted From Semen Oroxyli" by R. Y. Yan et al. that was published in a 2014 issue is presented.

  • Effect of 5-S-GAD on UV-B-induced cataracts in rats. Takahito Kimura; Shunji Natori // Japanese Journal of Ophthalmology;Sep2009, Vol. 53 Issue 5, p531 

    Abstract Purpose  5-S-Glutathionyl-N-β-alanyl-3,4-dihydroxyphenylalanine (5-S-GAD) is a novel antibacterial substance purified from Sarcophaga peregrina (flesh fly) that has both a radical scavenging activity and antioxidative activity. This is a report of an investigation of the effect...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics