High resolution pair-distance distribution function P(r) of protein solutions

Xinguo Hong; Quan Hao
February 2009
Applied Physics Letters;2/23/2009, Vol. 94 Issue 8, p083903
Academic Journal
A high resolution pair-distance distribution function P(r) of protein molecules has been obtained from the complete solution scattering curve made by combining accurate small-angle and wide-angle x-ray scattering data out to 2.15 Å resolution. Both indirect and direct Fourier transforms exhibit two distinct peaks at 1.4 and 5.1 Å in P(r). X-ray crystallographic data demonstrate that these peaks correspond to two intramolecular distances: the average bond length between C, N, and O atoms and the pitch of an α-helix, respectively. Hence some high resolution aspects of the structure and function of a protein may be investigated in a solution.


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