Gandhoke, I.; Aggarwal, R.; Hussain, S. A.; Pasha, S. T.; Sethi, P.; Thakur, S.; Lal, S.; Khare, S.
July 2009
Indian Journal of Medical Microbiology;Jul2009, Vol. 27 Issue 3, p222
Academic Journal
Background: Samples from babies exhibiting clinical symptoms suggestive of congenital infection are referred regularly to NICD, New Delhi,, from Government Hospitals located in Delhi and a home for abandoned children (Palna), for the diagnosis of etiological agents like toxoplasma, rubella, CMV and herpes. Blood samples of mothers of most of the affected babies are also received. Objective: Evaluation of rapid and accurate technique for the diagnosis of congenital CMV infection. Materials and Methods: One hundred and twenty five blood samples suggestive of symptomatic congenital CMV infection were selected from samples received at NICD during the period June 2005-March 2007. A request to collect and send the urine samples of the selected babies was sent to the respective hospitals. Serum samples of the babies were tested for CMV-IgM antibodies using μ-capture ELISA. Mothers' serum samples were subjected to CMV-IgM and IgG class antibodies assay by commercial ELISA kits. DNA isolation and amplification was performed in urine samples and some of the serum samples using a commercial PCR kit for detection of HCMV. Blood and urine samples from 20 normal babies were included in the study. Results: Twenty Seven serum samples (21.6%) of infants, of the 125 tested, were positive for CMV-IgM antibodies. Twenty five samples (20%) showed amplification of CMV -DNA. All 25 samples positive for PCR were positive for CMV IgM antibodies. Sera of 73 mothers, out of 75 tested (97.3%), were positive for CMV IgG antibodies. However, none of them was positive for CMV IgM antibodies. Mothers of all 27 positive babies were positive for CMV-IgG antibodies. Serum and urine samples from 20 normal babies were negative for ELISA and PCR. Conclusion: μ-capture ELISA technique was found to be more sensitive than PCR (92.6%) for detection of congenital CMV infection. ELISA is also rapid, less cumbersome and cost effective for diagnosis of CMV infection.


Related Articles

  • The Incidence of RSV Infection in the Cases Hospitalized with Bronchiolitis Diagnosis and the Relevant Clinical Particulars. Arica, Veik; Silfeler, Ibrahim; Onur, Hatice; Dağ, Hüseyin; Gülbayzar, Sayat; Obut, Ömer // Pediatrics & Therapeutics;2012, Vol. 2 Issue 4, p1 

    Aim: This study aims to investigate the incidence and clinical particulars of Respiratory Syncytial Virus (RSV) infection in infants hospitalized with bronchiolitis diagnoses between the years of 2006 and 2009. Material and method: From October 2006 to October 2009, the registers of a total of...

  • A sero-survey to identify the window of vulnerability to measles in infants in north-eastern Nigeria. Ahmadu, B. U.; Ashir, G. M.; Mustapha, M. G.; Mava, Y.; Saidu, I. H.; Ambe, J. P. // Southern African Journal of Epidemiology & Infection;2013, Vol. 28 Issue 3, p153 

    As infants lose maternal measles antibodies (MMAs), they experience periods when their antibody levels are insufficient to protect them against measles. A prospective study was carried out at the University of Maiduguri Teaching Hospital. Sera collected from neonates at birth, and at six weeks,...

  • Detección de infección asintomática por citomegalovirus en donadores voluntarios de sangre. Gutiérrez-Salinas, José; Carmona-García, Rosalba; García-Ortiz, Liliana; del Carmen Chima-Galán, María; Suástegui-Domínguez, Sigrit; Espinosa-Elizondo, Rosa Martha; de Jesús Treviño-Mejia, Alberto; García-Méndez, Sergio; Rivera-Badillo, Martha Elena // Medicina Interna de Mexico;mar/abr2010, Vol. 26 Issue 2, p109 

    Background: The infection by cytomegalovirus can appear in asymptomatic form and remain latent within the organism; reason why serologic and Molecular Biology studies as it is the PCR are important auxiliary diagnosys in this type of infections, mainly in the places where the people make a blood...

  • Isolation of cytomegalovirus and foamy virus from the drill monkey (Mandrillus leucophaeus) and prevalence of antibodies to these viruses amongst wild-born and captive-bred individuals. Blewett, E. L.; Lewis, J.; Gadsby, E. L.; Neubauer, S. R.; Eberle, R. // Archives of Virology;Feb2003, Vol. 148 Issue 3, p423 

    Summary. Drill monkeys (Mandrillus leucophaeus) are an endangered species whose indigenous viral flora is largely unknown. We report here the isolation and characterization of both a cytomegalovirus (DrCMV) and a foamy virus (SFV-drl) from drill monkeys. Phylogenetic analysis of DNA sequence...

  • Simultaneous Monitoring of Cytomegalovirus-Specific Antibody and T-cell levels in Seropositive Heart Transplant Recipients. Carbone, Javier; Lanio, Nallibe; Gallego, Antonio; Kern, Florian; Navarro, Joaquin; Muñoz, Patricia; Alonso, Roberto; Catalán, Pilar; Fernández-Yáñez, Juan; Palomo, Jesús; Ruiz, Manuel; Fernández-Cruz, Eduardo; Sarmiento, Elizabeth // Journal of Clinical Immunology;Aug2012, Vol. 32 Issue 4, p809 

    Purpose: Human cytomegalovirus (CMV) active infection (CMV infection) poses serious risks to CMV-seropositive heart transplant recipients. We evaluated the usefulness of simultaneous assessment of CMV-specific values for parameters of the humoral (antibodies) and cellular (CD4+ and CD8+ T-cells)...

  • HLA-DQ3 is a probable risk factor for CMV infection in high-risk kidney transplant patients. Marina Varga; Katalin Rajczy; Gábor Telkes; Márta Hídvégi; Antal Péter; Ádám Remport; Márta Korbonits; János Fazakas; Éva Toronyi; Enikõ Sárváry; László Kóbori; Jenõ Járay // Nephrology Dialysis Transplantation;Aug2008, Vol. 23 Issue 8, p2673 

    Background. Cytomegalovirus (CMV) infection in transplant patients with special risk factors remains a major hazard. CMV-seronegative recipients with seropositive donors have the highest risk of developing acute CMV disease. We suggest that the HLA-type may influence the occurrence and the...


    The research objective was to study frequency of antenatal infection and record probable clinical manifestations in 100 children with HCMV born from mother with HCMV in blood and mononuclear cells. The study identified poly-systemic internal organ damage in neonates due to prenatal HCMV....

  • Primary Cytomegalovirus Infection in Pregnant Egyptian Women Confirmed by Cytomegalovirus IgG Avidity Testing. Kamel, N.; Metwally, L.; Gomaa, N.; Sayed Ahmed, W.A.; Lotfi, M.; Younis, S. // Medical Principles & Practice;Dec2013, Vol. 23 Issue 1, p29 

    Objective: To determine the frequency of primary cytomegalovirus (CMV) infection in pregnant Egyptian women using CMV IgG avidity testing. Subjects and Methods: A cross-sectional study was conducted at Suez Canal University Hospital, Ismailia, Egypt. A total of 546 pregnant women, presenting for...

  • A Simple Method to Quantitate IP-10 in Dried Blood and Plasma Spots. Aabye, Martine G.; Eugen-Olsen, Jesper; Werlinrud, Anne Marie; Holm, Line Lindebo; Tuuminen, Tamara; Ravn, Pernille; Ruhwald, Morten // PLoS ONE;Jun2012, Vol. 7 Issue 6, p1 

    Background: Antigen specific release of IP-10 is an established marker for infection with M.tuberculosis. Compared to IFN-γ, IP-10 is released in 100-fold higher concentrations enabling the development of novel assays for detection. Dried blood spots are a convenient sample for high...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics