Colorimetric Method for Quantifying Human Stratum Corneum Removed by Adhesive-Tape-Stripping

Dreher, F.; Arens, A.; Hostýnek, J. J.; Mudumba, S.; Ademola, J.; Maibach, H. I.
June 1998
Acta Dermato-Venereologica;6/1/1998, Vol. 78 Issue 3, p186
Academic Journal
Tape-stripping of the skin is a useful method for removing the stratum corneum and obtaining more information about the function of this skin layer as the main barrier for skin penetration. The amount of stratum corneum removed is of relevance in establishing the concentration profile of chemicals within the stratum corneum after topical application. Weighing is the preferred method for measuring the amount stripped, but because it is often subject to artifacts, alternative methods are sought. We present a simple, colorimetric method for determining the amount stratum corneum removed by sequential adhesive-tape-stripping of human skin in vivo. The method is based on quantification of the sodium hydroxide soluble protein fraction using a commercially available protein assay similar to the Lowry assay. The method is shown to be an accurate and reproducible alternative to weighing, also demonstrating uniform removal of stratum corneum layers following the very initial strips.


Related Articles

  • Laser-induced alteration of contaminated papers. Rudolph, P.; Ligterink, F. J.; Pedersoli Jr., J. L.; Scholten, H.; Schipper, D.; Havermans, J. B. G. A.; Aziz, H. A.; Quillet, V.; Kraan, M.; Van Beek, B.; Corr, S.; Hua-Ströfer, H.-Y.; Stokmans, J.; Van Dalen, P.; Kautek, W. // Applied Physics A: Materials Science & Processing;2004, Vol. 79 Issue 4-6, p941 

    Cleaning of paper objects represents one of the most complex cases of laser ablation, since low volumes of dispersed material phases are evaporated while a sensitive and fragile fibrous organic matrix has to be preserved. Conventional chemical and mechanical cleaning methods suffer from the...

  • Identification of nest predators by photography, dummy eggs, and adhesive tape Major, Richard E. // Auk (American Ornithologists Union);Jan1991, Vol. 108 Issue 1, p190 

    No abstract available.

  • What if I could get... Ritter, George W. // Adhesives & Sealants Industry; 

    Opinion. Discusses the necessity of pressure-sensitive adhesive (PSA) tapes. Benefits of using adhesives; Disadvantages of using PSAs; What can be done with PSA.

  • PSA products may approach $9.2 billion in sales by 2004. Soscia, Katherine // Adhesives & Sealants Industry; 

    Forecasts that the sales of pressure-sensitive adhesives can reach an estimated $9.2 billion by the year 2004. Why this speculation was made; Information on the market for pressure-sensitive and gummed products; Detailed information on the products' sales.

  • Silicone ruber tape.  // Machine Design;01/15/98, Vol. 70 Issue 1, p134 

    Presents information on the mox-tape which provides a self-fusing, cohesive insulating barrier which withstands the elements. Description of the uses of the tape; Information on the availability of the tape.

  • Act on 2003 Today, Advises Economic Expert. McDevitt, John // Adhesives & Sealants Industry; 

    Presents views expressed by the author, former chief economist with 3M Company at the Pressure Sensitive Tape Council meeting on the importance to act upon economic indicators of the tape industry to emerge a strong market position by the year 2003 and beyond. Indication of continued industrial...

  • Happy birthday, Electrical tape.  // Electric Perspectives;Jan/Feb97, Vol. 22 Issue 1, p8 

    Recognizes the importance of the invention of vinyl electrical tape. Analysis on what it would be like without electrical tape; Information about the first version of the vinyl tape; Reason for switching from white to black version of vinyl tape.

  • Structural bonding tape.  // Machine Design;09/11/97, Vol. 69 Issue 17, p112 

    Features the double-sided structural bonding tape from the 3M Industrial Tape and Specialties Division from Saint Paul. Material which the tape is made of; Description of the tape.

  • Adhesive withstands oil and contamination.  // Design News;7/21/97, Vol. 53 Issue 14, p86 

    States that Locite 243 single-component threadlocking adhesive stands up to oil and other components. Benefits of these properties to fasteners; Information on how the adhesive cures; Description of Locite 243.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics