Electrowetting on dielectric-based microfluidics for integrated lipid bilayer formation and measurement

Poulos, Jason L.; Nelson, Wyatt C.; Tae-Joon Jeon; Chang-Jin Kim; Schmidt, Jacob J.
July 2009
Applied Physics Letters;7/6/2009, Vol. 95 Issue 1, p013706
Academic Journal
We present a microfluidic platform for the formation and electrical measurement of lipid bilayer membranes. Using electrowetting on dielectric (EWOD), two or more aqueous droplets surrounded by a lipid-containing organic phase were manipulated into contact to form a lipid bilayer at their interface. Thin-film Ag/AgCl electrodes integrated into the device enabled electrical measurement of membrane formation and the incorporation of gramicidin channels of two bilayers in parallel.


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