High miR-196a levels promote the oncogenic phenotype of colorectal cancer cells

Schimanski, Carl Christoph; Frerichs, Kirsten; Rahman, Fareed; Berger, Martin; Lang, Hauke; Galle, Peter R.; Moehler, Markus; Gockel, Ines; Veysey, Martin J.
May 2009
World Journal of Gastroenterology;5/7/2009, Vol. 15 Issue 17, p2089
Academic Journal
AIM: To analyze the relevance of the microRNA miR-196a for colorectal oncogenesis. METHODS: The impact of miR-196a on the restriction targets HoxA7, HoxB8, HoxC8 and HoxD8 was analyzed by reverse transcription polymerase chain reaction (RT-PCR) after transient transfection of SW480 cancer cells. The miR-196a transcription profile in colorectal cancer samples, mucosa samples and diverse cancer cell lines was quantified by RT-PCR. Transiently miR-196a-transfected colorectal cancer cells were used for diverse functional assays in vitro and for a xenograft lung metastasis model in vivo. RESULTS: HoxA7, HoxB8, HoxC8 and HoxD8 were restricted by miR-196a in a dose-dependent and gene-specific manner. High levels of miR-196a activated the AKT signaling pathway as indicated by increased phosphorylation of AKT. In addition, high levels of miR-196a promoted cancer cell detachment, migration, invasion and chemosensitivity towards platin derivatives but did not impact on proliferation or apoptosis. Furthermore, miR-196a increased the development of lung metastases in mice after tail vein injection. CONCLUSION: miR-196a exerts a pro-oncogenic influence in colorectal cancer.


Related Articles

  • cir-ITCH Plays an Inhibitory Role in Colorectal Cancer by Regulating the Wnt/β-Catenin Pathway. Huang, Guanli; Zhu, Hua; Shi, Yixiong; Wu, Wenzhi; Cai, Huajie; Chen, Xiangjian // PLoS ONE;Jun2015, Vol. 10 Issue 6, p1 

    Noncoding RNAs (ncRNAs) are the dominant product of eukaryotic transcription. These products range from short microRNAs (miRNAs) to long intergenic noncoding RNAs (lincRNAs). Circular RNAs composed of exonic sequences represent an understudied form of ncRNA that was discovered more than 20 years...

  • Beclin 1 Expression is Closely Linked to Colorectal Carcinogenesis and Distant Metastasis of Colorectal Carcinoma. Mei-Ying Zhang; Wen-Feng Gou; Shuang Zhao; Xiao-Yun Mao; Zhi-Hong Zheng; Yasuo Takano; Hua-Chuan Zheng // International Journal of Molecular Sciences;Aug2014, Vol. 15 Issue 8, p14372 

    Beclin 1 participates in development, autophagy, differentiation, anti- apoptosis, neurodegeneration, tumorigenesis and cancer progression. The roles of Beclin 1 in colorectal carcinogenesis and its subsequent progression are still unclear. Here, the mRNA and protein expression of Beclin 1 were...

  • Alcohol induces cell proliferation via hypermethylation of ADHFE1 in colorectal cancer cells. Ji Wook Moon; Soo Kyung Lee; Yong Woo Lee; Jung Ok Lee; Nami Kim; Hye Jeong Lee; Jung Seon Seo; Jin Kim; Hyeon Soo Kim; Sun-Hwa Park // BMC Cancer;2014, Vol. 14 Issue 1, p875 

    Background The hypermethylation of Alcohol dehydrogenase iron containing 1 (ADHFE1) was recently reported to be associated with colorectal cancer (CRC) differentiation. However, the effect of alcohol on ADHFE1 hypermethylation in CRC is still unclear. Methods The methylation status and...

  • Up-regulation of miR-224 promotes cancer cell proliferation and invasion and predicts relapse of colorectal cancer. Guang-jun Zhang; He Zhou; Hua-xu Xiao; Yu Li; Tong Zhou // Cancer Cell International;2013, Vol. 13 Issue 1, p1 

    Background MicroRNAs (miRNAs) are small, non-coding RNAs that can function as oncogenes or tumor suppressors in human cancer. Abnormally expressed miR-224 was found to play a fundamental role in several types of cancer. The aim of this study was to investigate the prognostic and biological...

  • Role of microRNA-141 in colorectal cancer with lymph node metastasis. LI FENG; HONGQING MA; LIANG CHANG; XINLIANG ZHOU; NA WANG; LIANMEI ZHAO; JING ZUO; YUDONG WANG; JING HAN; GUIYING WANG // Experimental & Therapeutic Medicine;Nov2016, Vol. 12 Issue 5, p3405 

    The present study aimed to investigate the role of microRNA (miR)-141 in the pathogenesis of colorectal cancer (CRC). In total, 58 CRC patients were included in the present study. The mRNA and protein expression levels of mitogen-activated protein kinase 4 (MAP4K4) were detected by reverse...

  • DR3/LARD spliced mRNA variants' frequency in colorectal cancer. Utkin, O.; Starikova, V.; Perenkov, A.; Yanchenko, O.; Baryshnikov, A.; Novikov, V. // Molecular Biology;Sep2013, Vol. 47 Issue 5, p721 

    Many variants of the DR3/LARD death receptor mRNA are derived during alternative splicing. Different DR3/LARD mRNAs encode the membrane and soluble forms of the receptor, which perform different functions. The frequency of the spliced mRNA variants of DR3/LARD was assessed by RT-PCR in patients...

  • Oncogenic Role of miR-15a-3p in 13q Amplicon-Driven Colorectal Adenoma-to-Carcinoma Progression. de Groen, Florence L. M.; Timmer, Lisette M.; Menezes, Renee X.; Diosdado, Begona; Hooijberg, Erik; Meijer, Gerrit A.; Steenbergen, Renske D. M.; Carvalho, Beatriz // PLoS ONE;7/6/2015, Vol. 10 Issue 7, p1 

    Progression from colorectal adenoma to carcinoma is strongly associated with an accumulation of genomic alterations, including gain of chromosome 13. This gain affects the whole q arm and is present in 40%–60% of all colorectal cancers (CRCs). Several genes located at this amplicon are...

  • MicroRNA-92a Functions as an Oncogene in Colorectal Cancer by Targeting PTEN. Zhang, Guangjun; Zhou, He; Xiao, Huaxu; Liu, Zuoliang; Tian, Hongpeng; Zhou, Tong // Digestive Diseases & Sciences;Jan2014, Vol. 59 Issue 1, p98 

    Background: Our previous studies show that microRNA-92a (miR-92a) is overexpressed in colorectal cancer (CRC) and is thought to be correlated with the development of the cancer. However, its biological role in CRC remains poorly understood. Aims: The aim of the study was to determine the role of...

  • Translating tumor biology into personalized treatment planning: analytical performance characteristics of the Oncotype DX® Colon Cancer Assay. Clark-Langone, Kim M.; Sangli, Chithra; Krishnakumar, Jayadevi; Watson, Drew // BMC Cancer;2010, Vol. 10, p691 

    Background: The Oncotype DX® Colon Cancer Assay is a new diagnostic test for determining the likelihood of recurrence in stage II colon cancer patients after surgical resection using fixed paraffin embedded (FPE) primary colon tumor tissue. Like the Oncotype DX Breast Cancer Assay, this is a...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics