TITLE

Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significantly correlated with cytochrome P450 suppression

AUTHOR(S)
Xia Chen; Chang-Kai Sun; Guo-Zhu Han; Jin-Yong Peng; Ying Li; Yan-Xia Liu; Yuan-Yuan Lv; Ke-Xin Liu; Qin Zhou; Hui-Jun Sun; Lai, Vincent
PUB. DATE
April 2009
SOURCE
World Journal of Gastroenterology;5/21/2009, Vol. 15 Issue 15, p1829
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
AIM: To investigate the hepatoprotective activity of tea polyphenols (TP) and its relation with cytochrome P450 (CYP450) expression in mice.METHODS: Hepatic CYP450 and CYPb5 levels were measured by UV-spectrophotometry in mice 2 d after intraperitoneal TP (25, 50 and 100 mg/kg per day). Then the mice were intragastricly pre-treated with TP (100, 200 and 400 mg/kg per day) for six days before paracetamol (1000 mg/kg) was given. Their acute mortality was compared with that of control mice. The mice were pre-treated with TP (100, 200, and 400 mg/kg per day) for five days before paracetamol (500 mg/kg) was given. Hepatic CYP2E1 and CYP1A2 protein and mRNA expression levels were evaluated by Western blotting, immunohistochemical staining and transcriptase-polymerase chain reaction. RESULTS: The hepatic CYP450 and CYPb5 levels in mice of TP-treated groups (100, 200 and 400 mg/kg per day) were decreased in a dose-dependent manner compared with those in the negative control mice. TP significantly attenuated the paracetamol-induced hepatic injury and dramatically reduced the mortality of paracetamol-treated mice. Furthermore, TP reduced CYP2E1 and CYP1A2 expression at both protein and mRNA levels in a dose-dependent manner. CONCLUSION: TP possess potential hepatoprotective properties and can suppress CYP450 expression.
ACCESSION #
42834287

 

Related Articles

  • Management of paracetamol overdose: current controversies. Kozer, E.; Koren, G. // Drug Safety;May2001, Vol. 24 Issue 7, p503 

    Paracetamol (acetaminophen) is one of the most frequently used analgesics, and is the most commonly used substance in self-poisoning in the US and UK. Paracetamol toxicity is manifested primarily in the liver. Treatment with N-acetylcysteine (NAC), if started within 10 hours from ingestion, can...

  • Differences in Caffeine 3-Demethylation Activity among Inbred Mouse Strains: A Comparison of Hepatic Cyp 1a2 Gene Expression between Two Inbred Strains1. Casley, William L.; Menzies, Allan; Girard, Michel; Larocque, Lyse; Mousseau, Nicole; Whitehouse, Larry W.; Moon, Thomas W. // Fundamental & Applied Toxicology;1997, Vol. 40 Issue 2, p228 

    The 3-demethylation of caffeine can be used as an index of cytochrome P450 CYP1A2 activity in vivo. We compared the plasma levels of caffeine and the 3-demethylated metabolite, 1,7-dimethylxanthine, in six common inbred strains (A/J, P/J, BALB/cJ, C3H/HeJ, AKR/J, and SWR/J) and one inbred strain...

  • Characterization of the Effects of Musk Ketone on Mouse Hepatic Cytochrome P450 Enzymes. Stuard, Sharon B.; Caudill, Douglas; Lehman-McKeeman, Lois D. // Fundamental & Applied Toxicology;1997, Vol. 40 Issue 2, p264 

    Nitroaromatic musks, including musk ketone (MK; 2,6-dimethyl-3,5-dinitro-4-t-butylacetophenone), are chemicals used as perfume ingredients in household products, cosmetics, and toiletries. Musk xylene (MX; 1,3,5-trinitro-2-t-butylxylene), another nitromusk, is not genotoxic but has been reported...

  • H NMR-based metabolomic analysis of triptolide-induced toxicity in liver-specific cytochrome P450 reductase knockout mice. Liu, Xia; Xue, Xiang; Gong, Likun; Qi, Xinming; Wu, Yuanfeng; Xing, Guozhen; Luan, Yang; Xiao, Ying; Wu, Xiongfei; Li, Yan; Chen, Min; Miao, Lingling; Yao, Jun; Gu, Jun; Lin, Donghai; Ren, Jin // Metabolomics;Oct2012, Vol. 8 Issue 5, p907 

    Triptolide (TL) is an active component of Tripterygium wilfordii Hook. F which is used to treat autoimmune and inflammatory disease. However, a high incidence of adverse effects is often observed in clinic. Previously we have demonstrated that cytochrome P450s (CYPs) are involved in the...

  • Reduced acetaminophen-induced liver injury in mice by genetic disruption of IL-1 receptor antagonist. Ishibe, Takuya; Kimura, Akihiko; Ishida, Yuko; Takayasu, Tatsunori; Hayashi, Takahito; Tsuneyama, Koichi; Matsushima, Kouji; Sakata, Ikuhiro; Mukaida, Naofumi; Kondo, Toshikazu // Laboratory Investigation (00236837);Jan2009, Vol. 89 Issue 1, p68 

    Acetaminophen (APAP) induced increases in intrahepatic expression of interleukin (IL)-1α, IL-1β, and IL-1 receptor antagonist (IL-1ra), when administered intraperitoneally. These observations prompted us to define the pathophysiological roles of IL-1ra in APAP-induced liver injury....

  • Should a lower treatment line be used when treating paracetamol poisoning in patients with chronic alcoholism?: a case against. Dargan, P.I.; Jones, A.L.; Dargan, Paul I; Jones, Alison L // Drug Safety;Jul2002, Vol. 25 Issue 9, p625 

    The widespread practice of using a lower plasma paracetamol (acetaminophen) concentration threshold for the treatment of paracetamol poisoning in patients with chronic alcoholism has been introduced on the basis of anecdotal case reports. In animals, acute alcohol loading inhibits toxic...

  • Acetaminophen, Alcohol, and Cytochrome P-450. Black, Martin // Annals of Internal Medicine;Mar1986, Vol. 104 Issue 3, p427 

    Editorial. Provides information on acetaminophen, alcohol and cytochrome P-450. Therapeutic use of acetaminophen; Irony of acetaminophen hepatotoxicity; Effects of alcohol on cytochrome P-450.

  • The role of cytochrome-P450 inhibitors in the prevention of hepatotoxicity after paracetamol overdose in rats. Walubo, A.; Barr, S.; Abraham, A. M.; Coetsee, C. // Human & Experimental Toxicology;Jan2004, Vol. 23 Issue 1, p49 

    Despite the understanding that some cytochrome P450 isoforms are responsible for activation of paracetamol to the hepatotoxic metabolite, N-acetyl-p-benzoquinineimine (NAPQI), the use of enzyme inhibitors for prevention and/or treatment of paracetamol hepatotoxicity is still not well researched....

  • Tolérance du paracétamol. Graham, Garry G.; Scott, Kieran F.; Day, Richard O. // Drugs;2003 Special Issue 2, Vol. 63, p43 

    Paracetamol (acetaminophen) is a well-tolerated drug at therapeutic doses and this safety profile is a major factor in the very wide use of the drug. It is well known that paracetamol is converted by the hepatic cytochrome P450 system to reactive compounds. Less well known is that paracetamol is...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics