TITLE

Error models for immunoassays

AUTHOR(S)
Sadler, William A.
PUB. DATE
September 2008
SOURCE
Annals of Clinical Biochemistry;Sep2008, Vol. 45 Issue 5, p481
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: For nearly 20 years, we and others have used a three-parameter power function as a direct estimation error model for immunoassays. The main application is imprecision profile plots (after translating from variance to coefficient of variation) but other uses include weighting functions for regression analysis and variance stabilizing transformations. Although generally successful, the intrinsic monotonicity of the function means that it fails to describe small but distinct increases in variance that occasionally occur near the assay detection limit. Methods: A systematic comparison of five variance functions was undertaken, using randomly drawn samples from a large body of real immunoassay data. Results: Variance function accuracy (hence imprecision profile accuracy) can be markedly improved, particularly near the assay detection limit, by employing a pair of complementary three-parameter power functions, together with a constrained four-parameter function, which provides for a variance turning point. Conclusions: A set of rules, based on an objective goodness-of-fit statistic, can be used to automate presentation of the most appropriate function for any particular data-set. Flexibility is easily incorporated into the selection rules and is actually highly desirable to encourage ongoing evaluation with a wider variety of data. AWin32 computer program that performs the variance function estimation and plotting is freely available.
ACCESSION #
42513237

 

Related Articles

  • 25-Hydroxyvitamin D in Canadian adults: biological, environmental, and behavioral correlates. Greene-Finestone, L.; Berger, C.; Groh, M.; Hanley, D.; Hidiroglou, N.; Sarafin, K.; Poliquin, S.; Krieger, J.; Richards, J.; Goltzman, D. // Osteoporosis International;May2011, Vol. 22 Issue 5, p1389 

    Summary: We assessed vitamin D status and its correlates in the population-based Canadian Multicentre Osteoporosis Study (CaMos). Results showed that serum 25-hydroxyvitamin D levels <75 nmol/L were common. Given Canada's high latitude, attention should be given to strategies for enhancing...

  • Youth Physical Activity Resource Use and Activity Measured by Accelerometry. Maslow, Andréa L.; Colabianchi, Natalie // American Journal of Health Behavior;Mar/Apr2011, Vol. 35 Issue 2, p219 

    Objectives: To examine whether use of physical activity resources (eg, parks) was associated with daily physical activity measured by accelerometry. Methods: One hundred eleven adolescents completed a travel diary with concurrent accelerometry. The main exposure was self-reported use of a...

  • The Cumulative Risk of Tuberculin Skin Test Conversion for Five Years of Hospital Employment. Ktsanes, Virginia K.; Williams, William L.; Boudreaux, Virgis V. // American Journal of Public Health;Jan1986, Vol. 76 Issue 1, p65 

    Abstract. We studied tuberculin skin test conversions in a cohort of 550 new employees followed for five years after assignment to adult inpatient services at Charity Hospital at New Orleans, Louisiana. There were 17 known conversions, an overall five-year cumulative probability of converting of...

  • Test Case Prioritization: A Family of Empirical Studies. Elbaum, Sebastian; Malishevsky, Alexey G.; Rothermel, Gregg // IEEE Transactions on Software Engineering;Feb2002, Vol. 28 Issue 2, p159 

    To reduce the cost of regression testing, software testers may prioritize their test cases so that those which are more important, by some measure, are run earlier in the regression testing process. One potential goal of such prioritization is to increase a test suite's rate of fault detection....

  • INVESTIGATING THE RELATIONSHIP BETWEEN SOFTWARE DEFECT DENSITY AND COST ESTIMATION DRIVERS: AN EMPIRICAL STUDY. WEDYAN, FADI; BANI-SALAMEH, HANI; AL-AJLOUNI, WAJEEHA; AL-MANAI, SHIRIN // Journal of Theoretical & Applied Information Technology;12/31/2015, Vol. 82 Issue 3, p446 

    This paper reports the results of an empirical study, where some hypotheses about the relationship between software quality, expressed in terms of defect density, and cost estimation drivers (cost factors) are assessed. The study is performed using three cost factors, these are: work effort,...

  • Cortisol levels in pregnancy as a psychobiological predictor for birth weight. Bolten, Margarete I.; Wurmser, Harald; Buske-Kirschbaum, Angelika; Papoušek, Mechthild; Pirke, Karl-Martin; Hellhammer, Dirk // Archives of Women's Mental Health;Feb2011, Vol. 14 Issue 1, p33 

    ntenatal maternal stress is thought to negatively affect fetal development, birth outcomes, and infant's development. Glucocorticoids are suggested to be a common link between prenatal stressors and infant's health. However, data on these mechanisms are rare and sometimes conflicting. The...

  • Relationship Exists between Vitamin D Levels and Serum Androgen Levels in Men.  // Fertility Weekly;1/10/2011, p2 

    The article presents a study which investigates the impact of Vitamin D on testosterone levels and hypopogonadism. Data from the Ludwigshafen Risk and Cardiovascular Health (LURIC) were utilized which included 2310 men and 1006 women routinely referred for coronary angiography. Methods used...

  • Non-normal variation and regression to the mean. Chesher, A. // Statistical Methods in Medical Research;1997, Vol. 6 Issue 2, p147 

    Non-normal variation across repeated measurements leads to nonlinear and heteroskedastic regression to the mean unlike the simple linear and homoskedastic regression to the mean found in normal models. This paper investigates the nature of the regression to the mean phenomenon in non-normal...

  • Comments on 'Background stratified Poisson regression analysis of cohort data' by Richardson and Langholz, Radiat Environ Biophys 51(1): 15-22. Muirhead, Colin; Haylock, Richard // Radiation & Environmental Biophysics;Mar2013, Vol. 52 Issue 1, p155 

    A letter to the editor is presented in response to the article "Background stratified Poisson regression analysis of cohort data," by Richardson and Langholz in the 2012 issue of Radiation & Environmental Biophysics."

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics