TITLE

Self-formation of bilayer lipid membranes on agarose-coated silicon surfaces studied by simultaneous electrophysiological and surface infrared spectroscopic measurements

AUTHOR(S)
Hirano-Iwata, Ayumi; Oshima, Azusa; Onodera, Kota; Aoto, Kouji; Taira, Tasuku; Yamaguchi, Ryo-taro; Kimura, Yasuo; Niwano, Michio
PUB. DATE
June 2009
SOURCE
Applied Physics Letters;6/15/2009, Vol. 94 Issue 24, p243906
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Self-formation process of bilayer lipid membranes (BLMs) cushioned on agarose-coated Si surfaces was in situ monitored by simultaneous electrophysiological and infrared absorption spectroscopic (IRAS) measurements using IRAS with the multiple internal reflection geometry. IRAS signals corresponding to self-thinning of lipid solution to form BLMs were demonstrated. It was found that the appearance of IRAS bands due to C=O modes of phosphstidylcholine is related to formation of BLMs with a gigaohm seal. The functionality of the present BLM system was also demonstrated by incorporating gramicidin into the BLMs and recording its channel activities.
ACCESSION #
42223765

 

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