TITLE

Effect of solution viscosity on generating long DNA nanostrands array via patterned molecular combing

AUTHOR(S)
Jun-Jie Cheng; Yuan-Hsin Chang; Wan-Yu Huang; Yi-Je Juang
PUB. DATE
June 2009
SOURCE
Applied Physics Letters;6/15/2009, Vol. 94 Issue 24, p244101
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In this study, we have investigated the effect of solution viscosity on generating the aligned and long DNA nanostrands array via modified molecular combing/soft lithography technique (or patterned molecular combing). By using the solution with higher viscosity, a low peeling-off speed can be applied to generate the long DNA nanostrands and the thornlike structures can be eliminated effectively. The DNA nanostrands array can also be generated by utilizing the polydimethylsiloxane stamp having microwells with larger diameter; however, variation of the lengths of the DNA nanostrands becomes greater as the solution viscosity and the size of microwells increase.
ACCESSION #
42223728

 

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