TITLE

Deep hypothermia impact on acid-base parameters and liver antioxidant status in an in vivo rat model

AUTHOR(S)
Alva, Norma; Carbonell, Teresa; Palomeque, Jesús
PUB. DATE
June 2009
SOURCE
Canadian Journal of Physiology & Pharmacology;Jun2009, Vol. 87 Issue 6, p471
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Although clinical hypothermia is used for reducing postischemic damage, injurious effects have also been reported. To determine whether hypoxia and oxidative stress are induced by systemic deep hypothermia, we used an in vivo rat model keeping the arterial Pco2 constant. Animals were divided into 4 groups: sham, 2 h deep hypothermia (21 °C), 1 h posthypothermia (rewarmed to 37 °C after 2 h deep hypothermia), and 3 h normothermia. Blood gases, portal vein blood flow, arterial pressure, and heart rate were monitored throughout the experiment. Liver enzyme antioxidant activity was also examined. The hemodynamic parameters decreased drastically during hypothermia, but were fully restored after rewarming. No changes in hepatic antioxidant activity (catalase, glutathione peroxidase, and superoxide dismutase) were observed. The redox level in liver (GSH/GSSG ratio) was preserved in hypothermia but decreased when animals were rewarmed. ALT did not increase and no evidence of tissue hypoxia was detected in liver regarding the restricted flow during hypothermia. With the described protocol, deep hypothermia is regarded as an experimental safe model.
ACCESSION #
41682915

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics