Quantifying thermodynamics of collagen thermal denaturation by second harmonic generation imaging

Hovhannisyan, Vladimir A.; Su, Ping-Jung; Lin, Sung-Jan; Dong, Chen-Yuan
June 2009
Applied Physics Letters;6/8/2009, Vol. 94 Issue 23, p233902
Academic Journal
Time-lapse second harmonic generation (SHG) microscopy was applied for the extraction of thermodynamic parameters of collagen thermal denaturation. We found that at sufficiently high temperatures, temporal dependence of SHG intensity from the isothermal treatment of chicken dermal collagen was single exponential and can be modeled by the Arrhenius equation. Activation energy and the frequency factor of chicken dermal collagen thermal denaturation were determined using temporal decays of SHG intensity at different temperatures. Our results show that time-lapse, high temperature SHG imaging can be used to quantify kinetic properties of collagen thermal denaturation within a microscopic volume of 1 nl.


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